Preparation of antioxidant peptides from Moringa oleifera leaves and their protection against oxidative damage in HepG2 cells

Abstract

Moringa oleifera leaves are a kind of new food raw materials, rich in functional factors, M. oleifera leaves aqueous extract have antioxidant activity and M. oleifera leave protein is an important active ingredient in the aqueous extract. Numerous studies have shown that peptides have strong antioxidant activity. To reveal the antioxidant effects of M. oleifera (MO) leaves peptides, MO leave antioxidant peptides were isolated and prepared to clarify their antioxidant activity. MLPH1 (<1 kDa), MLPH3 (1~3 kDa), MLPH5 (3~5 kDa), and MLPH10 (5~10 kDa) fractions were obtained by the membrane ultrafiltration classification of MO leaves proteolytic hydrolysate (MLPH). MLPH1 was further separated by centrifugal filters, and the fraction separated by <1 kDa (MLPH1-1) was identified and analyzed by LC–MS/MS. The purpose of this study was to investigate the effect of MO leaves antioxidant peptide pretreatment on H2O2-treated HepG2 cells and to refine the antioxidant activity. The results showed that MLPH1 had the strongest antioxidant activity, and three MO leaves antioxidant peptides (LALPVYN, LHIAALVFQ, and FHEEDDAKLF) were obtained. The peptide with the sequence LALPVYN and a molecular weight of 788.44 Da had the strongest antioxidant activity. After 24 h of LALPVYN pretreatment, the cell viability and the CAT, GSH-Px, and SOD enzyme activity were significantly increased, and the MDA, ROS, and apoptosis rates were significantly decreased. These results provide a theoretical basis for further research on the antioxidant mechanism of MO leaves peptides.

Structural properties of Kudzu protein enzymatic hydrolysate and its repair effect on HepG2 cells damaged by H2O2 oxidation

“…After treatment with KP and KPH, the apoptosis rate of cells was significantly reduced, and the repair effect of KPH on cells was better than that of KP. This is similar to the results reported by Liang et al , 94 who showed that the antioxidant peptides from Moringa oleifera leaves separated using an ultrafiltration membrane have high antioxidant activity.…”

Peptides from Harpadon nehereus Bone Ameliorate Angiotensin II-Induced HUVEC Injury and Dysfunction through Activation of the AKT/eNOS and Nrf2 Pathway

“…It has been reported that antioxidant peptides could reduce oxidative stress by eliminating the ROS and enhance the antioxidant system of the cell . In this study, we found that two peptides, especially PG-7, could significantly eliminate the ROS and increase the activities of SOD, CAT, and GSH-Px in Ang II-induced HUVEC (Figures and ).…”

“…It has been reported that antioxidant peptides could reduce oxidative stress by eliminating the ROS and enhance the antioxidant system of the cell. 15 In this study, we found that two peptides, especially PG-7, could significantly eliminate the ROS and increase the activities of SOD, CAT, and GSH-Px in Ang II-induced HUVEC ( Figures 2 and 3 ). Our results demonstrated that peptides KA-8 and PG-7 protected the HUVEC from Ang II-induced injury through attenuating oxidative stress, which was also reflected in the improvement of the cell number and morphology after treatment with peptides ( Figure 1 ).…”

Phytochemical Profiling by UHPLC–Q-TOF/MS and Chemopreventive Effect of Aqueous Extract of Moringa oleifera Leaves and Benzyl Isothiocyanate on Murine Mammary Carcinogenesis

“…Similarly, Fitriana [ 71 ] demonstrated a potent free radical scavenging activity with an IC 50 of 49.30 μg/mL in the DPPH assay using the methanolic extract of Moringa oleifera leaves, while we observed a value of 66.66 ± 0.53 μg/mL ( Table 3 ). Additionally, a significant antioxidant potential of peptides derived from M. oleifera leaves has been reported [ 72 ].…”