Preparation of antibodies against TXR1 and construction of a new DNA tumor vaccine

Sun, Yuanjie; Zhang, Xiyang; Hu, Chenchen; Pan, Jingyu; Liu, Tianyue; Ding, Jiaqi; Han, Chenying; Huang, Yinan; Yang, Kun

doi:10.1016/j.intimp.2021.108505

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…In clinical practice, therapeutic agents to suppress tumors can be designed by identifying molecular targets known to have oncogenic effects [ 31 ] . With this in mind, we analyzed apaQTL-genes in cancer signaling pathways, somatic mutations and immune infiltration.…”

Section: Discussionmentioning

confidence: 99%

Alternative polyadenylation-related genetic variants contribute to bladder cancer risk

Liu¹,

Gu²,

Li³

et al. 2023

J Biomed Res

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Aberrant alternative polyadenylation (APA) events play an important role in cancers, but little is known about whether APA-related genetic variants contribute to the susceptibility to bladder cancer. Previous genome-wide association study performed APA quantitative trait loci (apaQTL) analyses in bladder cancer, and identified 17 955 single nucleotide polymorphisms (SNPs). We found that gene symbols of APA affected by apaQTL-associated SNPs were closely correlated with cancer signaling pathways, high mutational burden, and immune infiltration. Association analysis showed that apaQTL-associated SNPs rs34402449 C>A, rs2683524 C>T, and rs11540872 C>G were significantly associated with susceptibility to bladder cancer (rs34402449: OR = 1.355, 95% confidence interval [CI]: 1.159–1.583, P = 1.33 × 10 −4 ; rs2683524: OR = 1.378, 95% CI: 1.164–1.632, P = 2.03 × 10 −4 ; rs11540872: OR = 1.472, 95% CI: 1.193–1.815, P = 3.06 × 10 −4 ). Cumulative effect analysis showed that the number of risk genotypes and smoking status were significantly associated with an increased risk of bladder cancer ( P trend = 2.87 × 10 −12 ). We found that PRR13 , being demonstrated the most significant effect on cell proliferation in bladder cancer cell lines, was more highly expressed in bladder cancer tissues than in adjacent normal tissues. Moreover, the rs2683524 T allele was correlated with shorter 3′ untranslated regions of PRR13 and increased PRR13 expression levels. Collectively, our findings have provided informative apaQTL resources and insights into the regulatory mechanisms linking apaQTL-associated variants to bladder cancer risk.

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Section: Discussionmentioning

confidence: 99%

Alternative polyadenylation-related genetic variants contribute to bladder cancer risk

Liu¹,

Gu²,

Li³

et al. 2023

J Biomed Res

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“…193 Others pursue DNA-based vaccine approaches to fight drug resistance in cancer. 194 Adenoviral-based drug delivery is an option to deliver nucleic acids (reviewed by Sakurai et al 195 ); however, repeated administration could fail to induce www.moleculartherapy.org…”

Section: Review Conclusion and Outlookmentioning

confidence: 99%

COVID-19 mRNA vaccines: Platforms and current developments

2022

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“…These molecules are eliminated from the blood by adding different neutral salts (ammonium sulphide, sodium chloride, and sodium sulphide). The solubility of proteins decreases with increasing salt concentration [1]. At very high salt concentration, proteins become insoluble and precipitates.…”

mentioning

confidence: 99%

“…The euglobulin fraction of immunoglobulin is precipitated for storage. 50-60% of Ammonium sulfate used for the preparation of main IgM as shown in fig (1) [2]. Potassium phosphate buffer in 60 mM concentration is added in antiserum (20 mL) for dilution and incubated at 4°C, at pH 7.0 for 24 hours.…”

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confidence: 99%

A comprehensive review on preparation of pure immunoglobulins Authors

Shehzad¹,

Tayyab²,

Bibi³

et al. 2022

IJNMS

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Immunoglobulins are also known as antibodies. Plasma cells are responsible for the production of immunoglobulins. Beta cells are activated against a pathogenic attack and facilitated the formation of different types of immunoglobulins naturally. These immunoglobulins are also artificially synthesized by non-specific laboratorial techniques include fractionation precipitation, electrophoretic methods, gel filtration chromatography, ion exchange chromatography, hydrophobic chromatography and by the specific immuno- adsorbent methods. Third generation immunoglobulins are effectively used for therapeutic purpose against viral infections intravenously. Second generation immunoglobulins synthesis involved removal of anti-complement contaminants and IgG aggregates, through enzymatic degradation and chemical modification. The harmful effects of intravenous immunoglobulins can be reduced by using ultraviolet light, P-propiolactone, and pepsin at pH 4. Intravenous immunoglobulin drugs lead to anti-inflammatory and immunomodulatory effects in various infections. The mechanism of action of intravenous immunoglobulins is dependent on the binding between the Fc portion of injected IgG and target cell receptors. Radiolabeling is performed by two methods as in vivo, in which radiolabeled antibodies are incorporated into the body to bind with the antibodies and in vitro method, radioactive material is bound with already formed antibodies. This chapter highlighted the artificial methods adopted for production of radiolabeled immunoglobulins holding significant therapeutic and diagnostic applications.

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Preparation of antibodies against TXR1 and construction of a new DNA tumor vaccine

Cited by 3 publications

References 32 publications

Alternative polyadenylation-related genetic variants contribute to bladder cancer risk

Alternative polyadenylation-related genetic variants contribute to bladder cancer risk

COVID-19 mRNA vaccines: Platforms and current developments

A comprehensive review on preparation of pure immunoglobulins Authors

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