Preparation of antibodies against subclasses of human IgG

Goosen, Pierre; Beekhuizen, S. van; Droogh, Cynthia; Lange, Gerda de

doi:10.1016/0022-1759(81)90365-3

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…According to the supplier, these antisera were specific for their subclass in the passive hemagglutination assays with erythrocytes coated with IgG of the various subclasses and in passive hemagglutination inhibition assays with IgG of the relevant subclass. The method of their production has been reported (7). The specificity of these antisera has been verified by others with different techniques (15,17,21), including fluorescence methods (31,36).…”

mentioning

confidence: 81%

Immunoglobulin G subclasses of fluorescent anti-Treponema pallidum antibodies: evidence for sequential development of specific anti-T. pallidum immunoglobulin G responses in patients with early syphilis

Sluis¹,

Reede²,

Boer³

1986

J Clin Microbiol

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The development of immunoglobulin G (IgG) subclass-specific anti-Treponema pallidum antibodies during the course of syphilis in humans was studied with sera from 50 untreated male patients. The patients were divided into five diagnosis groups. In the fluorescent treponemal antibody test, which delineates the presence of cross-reacting antibodies, as well as specific antitreponema antibodies, IgGl, IgG2, and IgG3 subclass antibodies were already present during the seronegative primary stage. Specific antibodies, which were detected by the fluorescent treponemal antibody absorption test, were first present during the serotype-variable primary stage. These antibodies were almost exclusively of the IgGl and IgG3 subclasses. In later stages, antibodies of other subclasses were detectable. Titration of IgGl antitreponema antibodies in three electrophoretically different IgG fractions revealed an asymmetric distribution in these fractions during primary syphilis. The antibodies were largely confined to the most basic fraction during primary syphilis. A sudden change in the distribution was noted between the end of the primary stage and the secondary stage; an even distribution of IgGl antitreponema antibodies existed in the late latent stage. These findings confirm and extend previous results from our laboratory. The development of antibodies detected by both tests is discussed in terms of a sequential stimulation of the immune system due to the presence of an extracellular layer covering the treponemas or, alternatively, in terms of a suppression of the immune response during early syphilis.

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mentioning

confidence: 81%

Immunoglobulin G subclasses of fluorescent anti-Treponema pallidum antibodies: evidence for sequential development of specific anti-T. pallidum immunoglobulin G responses in patients with early syphilis

Sluis¹,

Reede²,

Boer³

1986

J Clin Microbiol

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“…However, immunization of a total 7 rabbits with the Fc fragments over a one-year period thereafter resulted in no production of anti-Gtm(x). It has been shown that IgG1 is very low immunogenic in rabbits, but that IgG1 subclass-specific antisera can be produced by immunization following induction of unresponsiveness to the other subclasses (Spiegelberg and Weigle, 1968;Giessen et al, 1974;Goosen et al, 1981). With this and our experience in mind, 8 rabbits were injected first with IgG2 protein to suppress the production of antibodies against the common antigens on the IgG molecule.…”

Section: Isolation Of Lggmentioning

confidence: 96%

“…Anti-Glm(x) serum was produced in the light of previous work (Giesen et al, 1974;Goosen et al, 1981). The rabbits were immune-paralyzed with an intravenous injection of 10 mg IgG2(n) from which aggregated protein had been removed by ultracentrifugation.…”

Section: Isolation Of Lggmentioning

confidence: 99%

An improved method for the production of antisera to G1m allotypes

Kishida

Tamaki

1983

Jap J Human Genet

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“…Since the production rate of anti-Glm(2) was very low, immunization to Glm(2) was performed by the methods for the production of subclass-specific antibodies (Spiegelberg and Weigle, 1968;Giessen et al, 1974;Goosen et al, 1981). The rabbits were immune-paralysed by an intravenous injection of 10 mg IGG2(23).…”

Section: Introductionmentioning

confidence: 99%

Preparation of anti-Gm antibodies and their application to enzyme-linked immunosorbent assay (ELISA) and immunoblotting for Gm typing

Kishida

1985

Jap J Human Genet

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Preparation of antibodies against subclasses of human IgG

Cited by 9 publications

References 4 publications

Immunoglobulin G subclasses of fluorescent anti-Treponema pallidum antibodies: evidence for sequential development of specific anti-T. pallidum immunoglobulin G responses in patients with early syphilis

Immunoglobulin G subclasses of fluorescent anti-Treponema pallidum antibodies: evidence for sequential development of specific anti-T. pallidum immunoglobulin G responses in patients with early syphilis

An improved method for the production of antisera to G1m allotypes

Preparation of anti-Gm antibodies and their application to enzyme-linked immunosorbent assay (ELISA) and immunoblotting for Gm typing

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