Preparation of anastrozole loaded PEG-PLA nanoparticles: evaluation of apoptotic response of breast cancer cell lines

Alyafee, Yusra; Alaamery, Manal; Bawazeer, Shahad; Almutairi, Mansour S; Alghamdi, Badr; Alomran, Nawaf; Sheereen, Atia; Daghestani, Maha H.; Massadeh, Salam

doi:10.2147/ijn.s151139

Cited by 20 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 7C shows the confocal microscopic images of intracellular uptake of NPs in U87 cells. The relative transfection of HA-PEG-PLGA-D/P and CD-PEG-PLGA-D/P NPs in CD44-positive U87 cells relative to CD44-negative HepG2 cells was calculated by equation (2), and the corresponding values were 9.2 and 30.1, respectively, which were much higher than lipofectamine 3.0. In other words, the transfection of HA-PEG-PLGA-D/P and CD-PEG-PLGA-D/P NPs in CD44-positive U87 cells was at least ninefold higher than in CD44-negative HepG2 cells.…”

Section: Cytotoxicity Of D/p-loaded Npsmentioning

confidence: 99%

“…Poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) copolymeric nanoparticles (NPs) have been applied as drug and gene delivery nano-carriers with passive targeting character. 1,2 The PLGA-PEG copolymeric NPs possess amphiphilic conformation comprising hydrophobic PLGA core for drug encapsulation and hydrophilic PEG shell to avoid the reticuloendothelial system recognition. 3,4 These long-circulating NPs preferentially localize in the tumors from the leaky vasculature and the impaired lymphatic drainage due to enhanced permeability and retention effect.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery

Lin¹,

Lee²

2018

IJN

View full text Add to dashboard Cite

BackgroundHyaluronic acid (HA) and chondroitin sulfate (CD) are endogenous polysaccharides. In recent years, they have aroused the interest of scientists because of specific binding to CD44 receptors, which are overexpressed in several types of tumors.MethodsIn this study, HA- and CD-modified poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) copolymers were synthesized and applied to encapsulate 1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP)/pDNA (D/P) lipoplex as CD44 receptor targeting gene delivery nanoparticles (NPs).ResultsThe particle size of CD-PEG-PLGA-D/P (186.8 ± 21.7 nm) was smaller than that of HA-PEG-PLGA-D/P (270.2 ± 13.8 nm), with narrow size distribution, and both HA-PEG-PLGA-D/P NPs and CD-PEG-PLGA NPs possessed negative zeta potentials (−39.63 ± 5.44 mV and −38.9 ± 2.0 mV, respectively), which prevent erythrocytes from agglutination. Both NPs exhibited pH-dependent release and had faster release in pH 4.0 than in pH 7.4. Generally, the CD-PEG-PLGA-D/P NPs possessed less cytotoxicity than HA-PEG-PLGA-D/P NPs. The D/P-loaded HA-PEG-PLGA and CD-PEG-PLGA NPs expressed significantly higher transfection in CD44 high-expressed U87 (30.1% ± 2.1% and 40.7% ± 4.3%, respectively) than in CD44-negative HepG2 (3.3% ± 1.5% and 1.4% ± 1.0%, respectively) (p < 0.001). It was revealed that the endocytosis of HA-PEG-PLGA-D/P NPs was majorly dominated by macropinocytosis and the endocytosis of CD-PEG-PLGA-D/P NPs was dominated by clathrin-mediated endocytosis pathway (p < 0.001).ConclusionThe high selectivity to CD44-positive U87 cancer cells and low cytotoxicity in L929 normal cells assured the promising potential of CD-PEG-PLGA NPs as gene delivery nano-carriers.

show abstract

Section: Cytotoxicity Of D/p-loaded Npsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery

Lin¹,

Lee²

2018

IJN

View full text Add to dashboard Cite

show abstract

“…In turn MCL-1 gene is a proapoptotic member of the BCL-2 family of proteins, which show features of apoptosis inhibitors. The results of the study showed that all three genes were over-expressed, which leads to the induction of cancer cell apoptosis and thus directly confirms the efficacy of capsule drug delivery to the cells [30].…”

Section: Nanotechnologymentioning

confidence: 55%

“…This system contributed to the prolonged release of the drug -up to 144 hours. This prolonged release is the result of affinity to the binding between the polymer and the drug and the polymer capacity to introduce the drug [30]. The three-block polymer used for the synthesis of nanoparticles has an amphiphilic character, which makes it suitable for incapsulating water-insoluble compounds, including anastrozole.…”

Section: Nanotechnologymentioning

confidence: 99%

Anastrozole as aromatase inhibitor – new approaches to breast cancer treatment in postmenopausal women

Piotrowska¹,

Piotrowska²

2019

Nowotwory. Journal of Oncology

View full text Add to dashboard Cite

Introduction .Anastrozole is a chemotherapeutic drug used to treat breast cancer in postmenopausal women. The condition for success of this type of treatment is the presence of estrogen receptors in cancer cells. Anastrozole belongs to strong-acting, nonsteroidal inhibitors of aromatase, which is responsible for androgen-estrogen conversion process. The metabolism of this drug is based on biotransformation both in phase I and II. Initially it metabolizes in the liver with cytochrome P450 isoenzymes, followed by a glucuronidation reaction catalyzed by the UGT1A4 enzyme. Material and method . In order to minimize the side effects, new therapeutic approaches are sought to increase the success of the therapy. Conclusions . Biodegradable nano-tools that deliver anastrozole to the target site with an increase in its half-life to 144 hours are a promising method. A similar effect can be achieved by using a transdermal anastrozole administration system, which is a completely non-invasive method of treatment. NOWOTWORY J Oncol 2019; 69, 1: 26-35

show abstract

“…Beyond its use as an effective antineoplastic drug, anastrozole has been associated with increased rate of bone loss, also correlated with aromatase inhibition (AIBL), as well as several other side effects . The conjugation of anastrozole with a drug nanocarrier system is expected to decrease its side effects due to a better targeting strategy, which can also potentially enhance its efficiency …”

Section: Introductionmentioning

confidence: 99%

A proposal for the adsorption of anastrozole anticancer drug on gold nanoparticle surfaces

Teixeira

Costa

Sant’Ana

2019

J Raman Spectroscopy

View full text Add to dashboard Cite

Anastrozole is a well‐known anticancer drug used in aromatase‐dependent cancer chemotherapy as a nonsteroidal aromatase inhibitor. Investigations involving the adsorption of anastrozole on gold surfaces are on demand, because gold nanoparticles (AuNPs) have been used as nanocarriers of drugs, and such a compound can be used against aromatase‐expressing neoplastic cells. In this work, surface‐enhanced Raman scattering (SERS) spectroscopy was used to investigate the interaction between anastrozole and the surfaces of AuNPs. Density functional theory (DFT) calculations were used to simulate the molecular interactions with gold atom through two possible ways, triazole or nitrile moieties. Experimental results, supported by DFT calculations to assign main vibrational modes, indicate that the nitrile groups are the most preferred anchorage moieties for the adsorption.

show abstract

Preparation of anastrozole loaded PEG-PLA nanoparticles: evaluation of apoptotic response of breast cancer cell lines

Cited by 20 publications

References 32 publications

Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery

Polysaccharide-modified nanoparticles with intelligent CD44 receptor targeting ability for gene delivery

Anastrozole as aromatase inhibitor – new approaches to breast cancer treatment in postmenopausal women

A proposal for the adsorption of anastrozole anticancer drug on gold nanoparticle surfaces

Contact Info

Product

Resources

About