Preparation of an ultrafiltration membrane from the copolymer of acrylonitrile–glycidylmethacrylate utilized for immobilization of glucose oxidase

Godjevargova, Tzonka; Konsulov, Vitan; Dimov, A.

doi:10.1016/s0376-7388(98)00222-1

Cited by 49 publications

(28 citation statements)

References 13 publications

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“…6 The main advantages of this polymer include the chemical stability from the sturdy backbone of polyacrylonitrile and the optimum functionality, that is, further reacting ability owing to presence of the free and active epoxy group on GMA. The latter possibility offers the opportunity in a variety of activation/coupling chemistries for the covalent binding of capturers.…”

Section: Resultsmentioning

confidence: 99%

“…6,12 Figure 3 shows a schematic sketch of the functionalization process illustrating how BSA ligands can concurrently bond to two adjacent nanofibers, thereby form a chemical cross-linking, which is expected to improve the mechanical and thermal stabilities of the membrane.…”

Section: Resultsmentioning

confidence: 99%

“…Microfiltration (MF) range pore size of ENMs makes them efficient in removing relatively coarse particles and suspended solids but not tiny substances smaller than the pore size. [3][4][5][6][7] Considering the importance of separation of nanoparticles also organics that can be detrimental to the quality of water systems, optimizing the selectivity of such membranes could be crucial. Accordingly, not only the high permeability of the membrane is preserved but also on the basis of selectivity, the application domain would be extended from MF to ultrafiltration (UF) and even nanofiltration.…”

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confidence: 99%

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Biofunctionalized nanofibrous membranes mimicking carnivorous plants

Homaeigohar

Disci-Zayed²,

Dai

et al. 2013

Bioinspired, Biomimetic and Nanobiomaterials

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A novel biofunctionalized nanofibrous membrane is developed through immobilization of protein ligands on the surface of nanofibers. The biofunctionalization not only enhances the membrane's structural properties including mechanical and thermal ones but also makes the membrane capable to separate nanoparticles and biomolecules much smaller than the pore size from water efficiently. Upon contact with water, the conformational change of the protein immobilized leads to its swelling, thereby an enlarged functional surface area and a higher steric hindrance capturing the filtrates. In case of filtration of a plasmonic nanoparticle containing suspension, decoration of the membrane with the plasmonic nanoparticles forms a smart bionanocomposite biosensor for detection of protein denaturation. IntroductionMembrane technology for water treatment is steadily gaining very high importance worldwide. This is primarily due to water pollution and dwindling fresh water supplies leading to water scarcity. Water quality has to be controlled to ensure a safer environment by implementing efficient technologies such as advanced membranes offering more output with less input, that is, efficient energy saving membranes. Electrospun nanofibrous membranes (ENMs) that have the potential to be used as advanced membrane systems will be able to remove pollutants from the environment at lower energy and hence cost.1 Energy saving by ENMs derives from their high interconnected porosity leading to a very high permeability.2 Despite an extraordinary permeability, ENMs suffer from low size selectivity. Microfiltration (MF) range pore size of ENMs makes them efficient in removing relatively coarse particles and suspended solids but not tiny substances smaller than the pore size. [3][4][5][6][7] Considering the importance of separation of nanoparticles also organics that can be detrimental to the quality of water systems, optimizing the selectivity of such membranes could be crucial. Accordingly, not only the high permeability of the membrane is preserved but also on the basis of selectivity, the application domain would be extended from MF to ultrafiltration (UF) and even nanofiltration. Long-term functionality, that is, longevity of ENMs with regard to their extraordinary surface area thereby a higher exposed surface to the water streams is dependent on their mechanical stability. Hence, to maximize the efficiency of an ENM, besides the optimization of the selectivity, its mechanical stabilization should be also stressed.Here, we show that through one-single approach, that is, protein functionalization, an ENM can acquire mechanical and thermal stability while showing more optimum selectivity. Inspired by the

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Biofunctionalized nanofibrous membranes mimicking carnivorous plants

Homaeigohar

Disci-Zayed²,

Dai

et al. 2013

Bioinspired, Biomimetic and Nanobiomaterials

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“…The maximum current response was obtained at pH 6.5, and this pH effect was similar to the optimal pH range of 5.0 -6.0 reported for the free GOx. 25 Therefore, PBS at pH 6.5 was selected as the electrolyte in subsequent experiments.…”

Section: Optimization Of Experimental Parametersmentioning

confidence: 99%

Development of Highly Sensitive Amperometric Biosensor for Glucose Using Carbon Nanosphere/Sodium Alginate Composite Matrix for Enzyme Immobilization

Han

Cai

et al. 2014

ANAL. SCI.

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In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix. This hybrid material combined the advantages of CNS and natural biopolymer SA. This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase. Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature. Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10 -6 to 4.6 × 10 -3 M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3. Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.

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“…The maximum current response is obtained at pH 6.5, which is in agreement with the pH value of 5 -6 reported for native GOD. 35,36 Therefore, PBS at pH 6.5 was selected as the electrolyte in subsequent experiments.…”

Section: Optimization Of Detection Test Variablesmentioning

confidence: 99%