Preparation of an Electrically Conductive Graphene Oxide/Chitosan Scaffold for Cardiac Tissue Engineering

Jiang, Lili; Chen, Daoyu; Wang, Zhen; Zhang, Zhongmin; Xia, Yangliu; Xue, Huadan; Liu, Yong

doi:10.1007/s12010-019-02967-6

Cited by 96 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It typically mimics the extracellular matrix (ECM) for maximizing tissue regeneration by providing physicochemical and biomolecular cues to the cells. 19 Graphene and its derivatives are being explored for preparing scaffolds for bone, 20 cartilages, 21 cardiac, 22 and neural tissues, 23 among others. As discussed above, PEG modification of GO can markedly enhance its biodistribution and stability as well as biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

<p>Poly(Ethylene Glycol) Functionalized Graphene Oxide in Tissue Engineering: A Review on Recent Advances</p>

Ghosh¹,

Chatterjee²

2020

IJN

View full text Add to dashboard Cite

Owing to the unique physical, chemical, mechanical and electrical properties, graphene and its derivatives have been extensively researched for diverse biomedical applications including in tissue engineering since the past decade. Tunable chemical functionalities of graphene oxide (GO), a graphene derivative, allow easy surface functionalization. Functionalization of GO with poly(ethylene glycol) (PEG) (PEG-GO) has received significant attention as it offers superior solubility, stability, and biocompatibility. Besides being an attractive candidate for drug delivery, PEG-GO can aid in the attachment, proliferation, and differentiation of stem cells, thereby augmenting tissue engineering. PEG-GO has shown excellent antibacterial efficacy, which could be an added advantage to minimize implantassociated infections. This review describes the synthesis techniques, properties, and biological potential of PEG-GO towards mammalian and bacterial cells. Studies wherein these nanomaterials have been explored for engineering various tissues are reviewed along with future opportunities in this field.

show abstract

Section: Introductionmentioning

confidence: 99%

<p>Poly(Ethylene Glycol) Functionalized Graphene Oxide in Tissue Engineering: A Review on Recent Advances</p>

Ghosh¹,

Chatterjee²

2020

IJN

View full text Add to dashboard Cite

show abstract

“…depending on the application need. 14 Thus, CS biocomposites can be used in areas such as food packaging, 2,6 adsorption, 15,16 energy conversion, 9,17 sensor, [18][19][20] tissue engineering [21][22][23][24] and drug delivery. 25,26 The main objective of this study is to prepare conductive, mechanically developed, UV-protecting CS/GNP and CS/MWCNT biocomposite films in a simple and completely eco-friendly method.…”

Section: Introductionmentioning

confidence: 99%

Electrical, optical and mechanical properties of chitosan biocomposites

Mergen

Arda

Evingür

2019

Journal of Composite Materials

View full text Add to dashboard Cite

In this work, chitosan/graphene nanoplatelets (CS/GNP) and chitosan/multi-walled carbon nanotube (CS/MWCNT) biocomposite films were prepared using a simple, eco-friendly and low-cost method. The electrical, optical and mechanical properties of these composite films were investigated. The optical, mechanical and electrical properties of the biocomposites were significantly improved, which make them promising materials for food packaging, ultraviolet protection and biomedical applications. With the increase of carbon filler content (GNP or MWCNT) in CS biocomposites, the surface conductivity ( σ), the scattered light intensity ( I sc) and the tensile modulus ( E) increased significantly. This behaviour in the electrical, optical and mechanical properties of the CS/carbon filler biocomposites was explained by percolation theory. The electrical percolation thresholds were determined as R σ = 25.0 wt.% for CS/GNP and R σ = 10.0 wt.% for CS/MWCNT biocomposites, while the optical percolation thresholds were found as R op =12.0 wt.% for CS/GNP and R op = 2.0 wt.% for CS/MWCNT biocomposites. Conversely, the mechanical percolation thresholds for both CS/GNP and CS/MWCNT biocomposites were found to be negligibly small ( R m = 0.0 wt.%). The electrical ( β σ), optical ( β op) and mechanical ( β m) critical exponents were calculated for both CS/carbon filler biocomposites and found compatible with the applied percolation theory.

show abstract

“…Figure 7 shows the adhesion of H9C2 cells on the surface of hydrogel at different magnifications after 48 h. Figure 7(a) indicates a significant number of spherical cells, which were more obvious by increasing in the magnification in Figure 7(b). Moreover, Figure 7(c),(d) show the cell‐generated filopodia indicating the potential of hydrogel for appropriate cells' interactions 59 . As the micrographs were taken after 2 days, it seemed that the cells did not have enough time to expand on the surface, but the cells formed a uniform matrix even after 2 days.…”

Section: Resultsmentioning

confidence: 99%

An innovative approach to fabricate a thermosensitive melatonin‐loaded conductive pluronic/chitosan hydrogel for myocardial tissue engineering

Torabi

Mehdikhani

Varshosaz

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

This study aimed at designing and fabrication of a novel injectable and thermosensitive melatonin‐loaded pluronic/chitosan hydrogel containing gold nanoparticles (GNPs) and poly glycerol sebacate (PGS) for myocardial tissue engineering. The PGS nanoparticles were used as the melatonin (drug model) carrier. The gelation time, syringeability, stability, and swelling of the hydrogel were scrutinized. Rheological properties, chemical composition, and morphology of the samples were also investigated. The effect of GNPs addition on the electrical conductivity of hydrogel was assessed. The cytotoxicity of hydrogels was assessed through MTT assay in the exposure of H9C2 cells up to 7 days. Scanning electron microscopy was applied to evaluate the morphology of seeded cells. The synthesis parameters of PGS nanoparticles were optimized through which 2.5%w/v of PGS and 1:10 organic phase to aqueous phase (O/A) ratio were found desirable. The optimum hydrogel illustrated 2 min gelation time and was stable up to 20 days with 5% swelling in the first 12 h into phosphate buffered saline. The GNPs with a uniform distribution rendered the hydrogel electrically conductive (1500 μS/cm). According to the MTT assay results, 3.125 μM melatonin was considered as the suitable concentration by which a significant increase in the cell viability was observed. The results exhibited that the prepared hydrogel composed of pluronic/chitosan/GNPs, and 3.125 μM melatonin‐loaded PGS nanoparticles could be applied as a promising scaffold for myocardial tissue engineering.

show abstract

Preparation of an Electrically Conductive Graphene Oxide/Chitosan Scaffold for Cardiac Tissue Engineering

Cited by 96 publications

References 33 publications

<p>Poly(Ethylene Glycol) Functionalized Graphene Oxide in Tissue Engineering: A Review on Recent Advances</p>

<p>Poly(Ethylene Glycol) Functionalized Graphene Oxide in Tissue Engineering: A Review on Recent Advances</p>

Electrical, optical and mechanical properties of chitosan biocomposites

An innovative approach to fabricate a thermosensitive melatonin‐loaded conductive pluronic/chitosan hydrogel for myocardial tissue engineering

Contact Info

Product

Resources

About