“…Additives have extensively been developed as synthetic cryoprotectants that are expected to eliminate the concerns above related to the long-term low temperature storage of biologics including proteins and cells. − Cell recovery from cryopreservation is significantly enhanced by using cryoprotectants, such as carboxylated ε-poly( l -Lys), graphene oxide, block copolymer/poly(vinyl alcohol) (PVA) composite systems, oligo( l -Pro), amphiphilic poly( l -Ala) block copolymers, amphiphilic poly( N , N -dimethyl acrylamide- co -alkyl acrylamide), and low molecular weight poly(ethylene glycol)s (PEGs). − The enhanced cell recovery with those cryoprotectants mainly results from ice recrystallization inhibition (IRI) and relief of the osmotic stress inside or outside of cells. Coacervates were prepared by liquid–liquid phase separation for the dodecyl trimethylammonium bromide/5-methyl salicylate, sodium dodecyl sulfate/1,2-diphenylethylenediamine, oleic acid/spermine, and polyethylene glycol/sodium citrate .…”