Preparation of Alginate/Poly(L-Arginine)-Chitosan Ternary Complex Microcapsules

Chen, A.Z.; Wang, Shi Bin; Weng, Lian-Jin; Chen, M.Y.

doi:10.4028/www.scientific.net/jbbte.3.25

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…5 has shown the release profile of indomethacin in pH 2.1, 7.4 PBS at 37 o C. It was found that only 22.3 % of indomethacin was released from the beads at pH=2.1 PBS, whereas 60.1 % indomethacin was diffused into pH=7.4 PBS within 500 mins. The same tendency of drug release in different pH PBS was also reported in other drug release system composed of alginate/poly(L-arginine)-chitosan microcapsules [7] and N-carboxymethyl chitosan beads [8]. In pH=2.1 PBS, the ionization of carboxyl groups is very low, so the H-bonding among polymeric network derived from NH and COOH groups was very strong.…”

supporting

confidence: 74%

Drug Release Behaviors of a pH/Temperature Sensitive Hydrogel Bead with Core-Shelled Structure

Deng

Dong

Shi

et al. 2010

AMR

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As a drug delivery carrier, a novel pH/temperature sensitive bead (pTSB) with core-shelled structure from poly(N-acryloylglycine) (PAG), copoly(N-acryloylglycine methyl este and N-acryloylglycine ethyl ester) was prepared by two steps. In pH=7.4 phosphate buffer solution (PBS), the cumulative release amount of indomethacin loaded in the pTSB was about 60.1 % within 500 mins, but this value only reached to 22.3 % in pH=2.1 PBS. The release behaviors of indomethacin from pTSB also exhibited a remarkable dependence on PAG content in the core. Additionally, the release rate of indomethacin was much faster at 18 oC than that at 37 oC due to the temperature sensitivity of poly(N-acryloylglycinates). The experimental results indicate that pTSB seems to have a potential application in the drug release system controlled via pH or temperature in the biomedical fields.

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supporting

confidence: 74%

Drug Release Behaviors of a pH/Temperature Sensitive Hydrogel Bead with Core-Shelled Structure

Deng

Dong

Shi

et al. 2010

AMR

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“…The expected strong nuclear uptake of pArg containing SmacN7 conjugate mediated cell death in tumor and healthy cells [96]. Ternary complex microcapsules of alginate/pArg-chitosan were fabricated by coating chitosan and pArg as membrane materials on calcium alginate beads [97]. The investigation of the in vitro drug release alginate/pArg-chitosan microcapsules exhibit more sustained and stable macromolecular drug release compared with alginate/chitosan microcapsules.…”

Section: Types Of Ph-responsive Polypeptidesmentioning

confidence: 99%

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

et al. 2019

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Smart nano-carriers have attained great significance in the biomedical field due to their versatile and interesting designs with different functionalities. The initial stages of the development of nanocarriers mainly focused on the guest loading efficiency, biocompatibility of the host and the circulation time. Later the requirements of less side effects with more efficacy arose by attributing targetability and stimuli-responsive characteristics to nano-carriers along with their bio- compatibility. Researchers are utilizing many stimuli-responsive polymers for the better release of the guest molecules at the targeted sites. Among these, pH-triggered release achieves increasing importance because of the pH variation in different organ and cancer cells of acidic pH. This specific feature is utilized to release the guest molecules more precisely in the targeted site by designing polymers having specific functionality with the pH dependent morphology change characteristics. In this review, we mainly concert on the pH-responsive polypeptides and some interesting nano-carrier designs for the effective theranostic applications. Also, emphasis is made on pharmaceutical application of the different nano-carriers with respect to the organ, tissue and cellular level pH environment.

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“…Ponce's results [3] illustrate that the different observed immune responses to alginate microcapsules are the consequence of the variations in the interactions between the polycations and alginates rather than to the alginates themselves. At present, chitosan and poly-L-lysine (PLL) are widely used as polycationic coating materials for polyelectrolytical complex reactions [4][5][6][7][8]. However, a perfect encapsulation system requires a gel entrapment system which is chemically and mechanically stable as well as biocompatible both for the host and the entrapped cells [3,9], and some reports show that the mechanical strength and biocompatibility of PLL microcapsules need to be improved [7,8].…”

Section: Introductionmentioning

confidence: 99%

Molecular Biocompatibility Evaluation of Poly-L-Ornithine-Coated Alginate Microcapsules by Investigating mRNA Expression of Pro-Inflammatory Cytokines

Chen

Wang

Liu

et al. 2012

JBBTE

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Following a polyelectrolytical complex reaction, the poly-L-ornithine (PLO)-alginate microcapsules were prepared by coating PLO on calcium alginate beads which were produced by a high-voltage electrostatic droplet generator. The biocompatibility of the microcapsules at the molecular level was evaluated through investigating the mRNA expression of pro-inflammatory cytokines; that is, the effect of the PLO coating of alginate beads on the mRNA expression of TNF-α, IL-1β, and IL-6 were measured using the RT-PCR method. The resulting PLO-coated alginate microcapsules have a smooth surface with a mean diameter of 309µm. The molecular biocompatibility studies show that coating microcapsules with PLO has no significant effect on the biocompatibility of alginate microcapsules (p>0.05), and both alginate microcapsules and PLO-coated microcapsules are significantly different from the positive control (p<0.05); however, both are also capable of causing an inflammatory response at a molecular level since both are significantly different from the blank control (p<0.05). Furthermore, with the increase in concentration of microcapsules or co-cultured time, part of the mRNA expression of cytokines is significantly increased. The results also demonstrate that the method used in this study, co-incubating the microcapsules with macrophages and measuring the mRNA expression of cytokines by RT-PCR, may be a useful method for evaluating the biocompatibility of coating materials of microcapsules.

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Preparation of Alginate/Poly(L-Arginine)-Chitosan Ternary Complex Microcapsules

Cited by 5 publications

References 21 publications

Drug Release Behaviors of a pH/Temperature Sensitive Hydrogel Bead with Core-Shelled Structure

Drug Release Behaviors of a pH/Temperature Sensitive Hydrogel Bead with Core-Shelled Structure

pH-Responsive Polypeptide-Based Smart Nano-Carriers for Theranostic Applications

Molecular Biocompatibility Evaluation of Poly-L-Ornithine-Coated Alginate Microcapsules by Investigating mRNA Expression of Pro-Inflammatory Cytokines

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