Preparation of algal-oligosaccharide mixtures by bacterial agarases and their antioxidative properties

Abstract

Algal-oligosaccharide-lysates (AOL), derived from six agars and four algal polysaccharide extracts (APE), were treated with 100-500 activity units (AU) of MA103-agarases or MAEF108-agarases, and their antioxidative properties evaluated. Soluble total polyphenols (TP) were between 462.2 ± 1.6 gallic acid equivalents (GAE, mg/mL) and 70.6 ± 17.4 GAE. The DPPH radical scavenging capacity of all AOL went from 68.3 ± 0.7% to 0.5 ± 0.1%. The ferrous ion chelating capacity of all AOL went from 93.1 ± 0.2% to 21.7 ± 0.9%. Evaluation of the H 2 O 2 scavenging capacity of all AOL was between 35.9 ± 5.4% and 0.1 ± 0.2%. The reducing power of all AOL went from 51.3 ± 2.6 to 3.2 ± 6.8 expressed as mg/mL ascorbic acid. In DPPH radical scavenging capacity, ferrous ion chelating capacity and reducing power etc., the AOL derived from the APE of Porphyra dentate (digested by 500 AU of MAEF108-agarases) were highest, in all test sets. However, the AOL derived from the APE of Monostroma nitidum (digested by 500 AU of MAEF108-agarases) had the highest H 2 O 2 scavenging capacity in all test sets. The order of antioxidative activity performance of all AOL treated in this experiment, by these four antioxidative methods, is as follows: ferrous ion chelating capacity > DPPH radical scavenging capacity > H 2 O 2 scavenging capacity > reducing power; this may be related to their polyphenols, small molecular weight polysaccharides or simple sugar constituents. In this study, it is demonstrated that various agarases derived from algal oligosaccharide mixtures possess good potential for use as a health food, due to their antioxidative capacity.

Degradation of porphyran from Porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight

“…From the results, the decrease in DPPH-radical indicated the radical-scavenging effect of porphyrans. It is obvious that the scavenging effects obeyed the order of P 3 > P 2 > P 1 > P. Wu and Pan [6] had demonstrated the similar result. They attributed the increasing effect on scavenging DPPH-radical of agar polysaccharide extracts from Porphyra dontate to increased oligosaccharide production caused by increased agarase activity units.…”

“…Structurally, it has a linear backbone of alternating 3-linked ␤-d-galactosyl units and 4-linked ␣-l-galactosyl 6-sulfate or 3,6-anhydro-␣-l-galactosyl units [1]. Bioasssays have shown that porphyran could stimulate immune response [2,3], scavenging active oxygen radicals in vitro [4][5][6], increase antioxidant enzymes activity and antioxidant capacity in aging mice [7]. However, porphyran is a high molecular weight polysaccharide featuring high viscosity, which may limit its pharmaceutical application.…”

“…However, reports on porphyran degradation are scarce and mainly about agarase hydrolysis, such as ␤-agarase from Arthrobacter sp. S-22 [11] and crude agarses from Pseudomonas vesicularis MA 103 or Aeromonas salmonicida MAEF 108 [6].…”

Characterization and immunostimulating activity of a water-soluble polysaccharide isolated from Haematococcus lacustris

“…Immune modulating activity of HCP Expression of iNOS and COX-2 in macrophages is regulated by transcriptional activation during immune responses and inflammation [12,50,51]. Therefore, RT-PCR analysis was performed to evaluate the effects of purified HCP on macrophage activation by measuring the expression levels of iNOS and COX-2 mRNA from RAW264.7 cells.…”

Hydrolysis of Chlorella by Cellulomonas sp. YJ5 Cellulases and Its Biofunctional Properties

“…Compared with the 100 ppm Vitamin C, 20% Chlorella hydrolysates had higher TEAC and reducing power values ( P < 0.05). These phenomena suggested that, not only the hydrolyzed soluble proteins and peptides, but the released polysaccharides also have iron chelating and free radical scavenging abilities (Wu and Pan 2004; Yin and others 2007). Chlorella contains many dietary antioxidants that could eventually be responsible for some of its functional activities.…”