Preparation of agglutinating antisera specific for the flagellar antigens of Pseudomonas aeruginosa

Pitt, T. L.

doi:10.1099/00222615-14-3-251

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…Mares being mated after a difficult foaling are particularly vulnerable to infection (Hughes and Loy 1975), as is tissue damaged by a surgical procedure. The present study indicates that the most frequent P aeruginosa isolated was type 03, H3, which accounted for nearly 50 per cent of isolates, whereas Pitt (1981b) found an incidence of only 6.7 per cent in isolates from human cases. This may reflect a significant association of this serotype with infections in horses or, alternatively, its frequency of isolation may have been a consequence of the outbreaks.…”

Section: Discussioncontrasting

confidence: 43%

Types of Pseudomonas aeruginosa isolated from horses

Atherton

Pitt

1982

Equine Veterinary Journal

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Summary Pseudomonas aeruginosa isolates from equine clinical material were categorised according to their serotype and phage type. Epidemiological evidence showed that serotypes 02a, 03,04,06, 09 and 010 were the cause of genital and non‐genital infections; somatic type 03 accounted for 50 per cent of isolates. The laboratory tests used were of no value in predicting whether or not a particular isolate was likely to be a venereal pathogen, but all the serotypes encountered had the potential to be pathogenic, given a favourable environment in which to multiply. Résumé Des souches de Pseudomonas aeruginosa isolées de prélèvements cliniques équins ont été classées suivant leur sérotype et suivant leur type phage. L'étude épidémiologique a montré que les sérotypes 02a, 03, 04, 06, 09 et 010 étaient la cause d'infections génitales et extra génitales. Le type somatique 03 représentait 50% des souches isolées. Les épreuves de laboratoire utilisées parurent n'avoir aucune valeur pour la prévision de l'aptitude pathogène vénérienne des souches. Tous les sérotypes rencontrés parurent être des pathogènes potentiels, en function d'un environnement favorable à leur multiplication. Zussammenfassung Pseudomonas aeruginosa Isolate aus klinischem Material von Pferden wurden nach Serotyp und Phagentyp eingeteilt. Die epidemiologischen Gegebenheiten zeigten, dass die Serotypen 02a, 03, 04, 06, 09 und 010 die Ursache genitaler und nicht‐genitaler Infektionen sind; der somatische Typ 03 wurde in 50 Prozent der Isolate nachgewiesen. Die verwendeten Labortests erwiesen sich als nutzlos für die Beantwortung der Frage, ob ein bestimmtes Isolat als Erreger einer Genitalinfektion in Frage komme. Alle nachgewiesenen Serotypen erwiesen sich aber als potentiell pathogen, wenn sie ein für ihre Vermehrung günstiges Milieu antrafen.

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Section: Discussioncontrasting

confidence: 43%

Types of Pseudomonas aeruginosa isolated from horses

Atherton

Pitt

1982

Equine Veterinary Journal

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“…A laboratory reference strain of P. aeruginosa (international antigenic typing scheme (IATS) serotype 0:1 (National Collection of Type Cultures 11440, American Type Culture Collection 3348), nonmucoid, piliated and gentamycin-sensitive) was used [16]. The bacteria were grown on blood agar plates for four consecutive days before overnight culture in tryptone soya broth (Unipath Ltd, Basingstoke, UK) to ensure piliation.…”

Section: Bacterial Strainmentioning

confidence: 99%

Inflammation in cystic fibrosis airways: relationship to increased bacterial adherence

et al. 2001

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It is unclear whether in¯ammation in the cystic ®brosis (CF) lung relates predominantly to bacterial infection, or occurs as a direct consequence of mutant cystic ®brosis transmembrane conductance regulator (CFTR) protein.Interleukin (IL)-8 secretion from CF and non-CF cell lines, and from CF and non-CF human primary nasal epithelial cells incubated with or without Pseudomonas aeruginosa, was measured. Activation of nuclear factor-kB (NF-kB) in unstimulated CF and non-CF nasal epithelial cells, cell lines and murine tissues was measured by gelshift assays.No signi®cant difference in basal IL-8 production or NF-kB activation was observed between CF and non-CF primary nasal cells. However, CF cells exhibited a signi®cantly (p<0.01) increased IL-8 secretion following P. aeruginosa stimulation. Equalization of the increased P. aeruginosa adherence observed in CF cells, to non-CF levels, resulted in comparable IL-8 secretion. Further, IL-8 production did not differ with mutations which result in either correctly localized CFTR, or in partial/total mislocalization of this protein. Similar levels of NF-kB activation were observed in a number of organs of wildtype and CF mice. Finally, IL-8 secretion and NF-kB activity were not consistently increased in CF cell lines. Cos-7 cell transfection with plasmids expressing DF508 or G551D mutant CFTR protein resulted in increased activation of a p50-containing NFkB complex, but IL-8 secretion was similar to wild-type cells.The authors conclude that the stimulus produced by Pseudomonas aeruginosa is the predominant in¯ammatory trigger in their models. Eur Respir J 2001; 17: 27±35.

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“…Methods for the preparation and characterisation of H-specific antisera, and the origin of the H-type strains, were described in the preceding paper (Pitt, 1981). 0 antisera.…”

Section: Serological Typing Methodsmentioning

confidence: 99%

“…Pooled H sera were not used in routine typing. Strains that were not H typable on first testing were passed twice through nitrate-containing soft agar in Craigie tubes (Pitt, 1981) and plated on agar. Cultures were re-tested by slide agglutination with the H-sera diluted to approximately one-tenth of the tray-test dilutions.…”

Section: Serological Typing Methodsmentioning

confidence: 99%

A comparison of flagellar typing and phage typing as means of subdividing the O groups of Pseudomonas aeruginosa

Pitt¹

1981

Journal of Medical Microbiology

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SUMMARY.Strains of Pseudomonas aeruginosa from hospitals were typed by 0 and H serology and bacteriophage typing. The H sera were prepared against purified flagella of six type strains.The most common 0 serogroups were 0 6 , 0 1 1 , O 16 and 0 10, and together these groups represented more than half of the total number of strains. 0 subgrouping proved useful for the further division of groups 0 2 and 06.Percentage H typability was high, and many H patterns were found. Comparison of H typing and phage typing as a means of making subdivisions within 0 groups showed that, although the general discriminatory power of the two methods was similar, H typing performed better than phage-typing in the more common 0 serogroups.

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Preparation of agglutinating antisera specific for the flagellar antigens of Pseudomonas aeruginosa

Cited by 13 publications

References 22 publications

Types of Pseudomonas aeruginosa isolated from horses

Types of Pseudomonas aeruginosa isolated from horses

Inflammation in cystic fibrosis airways: relationship to increased bacterial adherence

A comparison of flagellar typing and phage typing as means of subdividing the O groups of Pseudomonas aeruginosa

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