Preparation of affinity membranes using polymer phase separation and azido-containing surfactants

Clickable polymer scaffolds enable Ce recovery with peptide ligands

“…The ability of the EF-hand peptide to bind cerium when attached to a polymer scaffold indicates that the secondary structure that facilitates binding can form. Overall, this study establishes a methodology for conjugating any engineered peptide to a polymer scaffold surface for use in adsorbing ions or other small molecules, whereas previous literature has focused on: enhancing biocompatibility, 14 mitigating biofouling, 61 drug capture, 62 and using short di-or tri-peptides for surface modifications. 14,62,63 By further designing peptides and elucidating their sequence-structure-function relationships, the applications of this technology are limitless and could be utilized to secure a domestic supply of recycled REEs.…”

Recent Developments of Liquid Chromatography Stationary Phases for Compound Separation: From Proteins to Small Organic Compounds

“…A similar cobalt-based system was also employed to capture bovine serum albumin using modified chitosan [ 53 ]. Furthermore, a combination of ions can also enhance selectivity to His-tagged proteins, for instance, copper(II) ions and nickel(II) ions that are co-immobilized on a crosslinked chitosan/polyvinyl alcohol support [ 54 ].…”

“…The immobilized ions are usually stabilized by chelating ligands, such nitrilotriacetic acid (NTA), which are covalently anchored to the stationary phases. Several strategies have been reported to efficiently incorporate NTA ligands into the membranes through click-chemistry to azido-containing cellulose acetate membranes [ 54 ]. Another example of chelating ligands is hydroxamic acid.…”

Molecularly Imprinted Affinity Membrane: A Review

“…A few techniques have been detailed to effectively introduce NTA ligands into the membranes, such as through click chemistry to azido-containing cellulose acetate membranes. 40 Hydroxamic acid is another example of a chelating ligand that can stabilize copper(II) ions anchored onto the poly(methyl methacrylate)-grafted cellophane membranes. 41 It was reported that there was no leakage of Cu 2+ ions observed when the hydroxamic acid–Cu 2+ system was eluted while eluting 88% of the absorbed His-tagged chitinase.…”

“…The chelating ligands are incorporated into the membrane by covalent interaction. A few techniques have been detailed to effectively introduce NTA ligands into the membranes, such as through click chemistry to azido-containing cellulose acetate membranes . Hydroxamic acid is another example of a chelating ligand that can stabilize copper­(II) ions anchored onto the poly­(methyl methacrylate)-grafted cellophane membranes .…”