Preparation of ADP-glucose with an enzyme from Arthrobacter simplex.

Abstract

For the purpose of enzymatic preparation of ADP-glucose (ADPG), bacterial screening was performed to find a strain having a high activity of ADPGpyrophosphorylase which catalyzes the synthesis of ADPGfrom ATPand glucose-1-phosphate. A cell-free extract of Arthrobacter simplex IFO 12069 showed a strong enzymeactivity for the synthesis ofADPG,which was isolated from the reaction solution by ion-exchange column chromatography and identified by paper and thin-layer chromatography. The enzyme activity of the bacterium reached a maximumin the late logarithmic phase under aerobic growth conditions. Somefactors affecting the ADPGsynthesis, e.g. reaction pH, substrate concentrations, divalent cations, inhibitors and activators, were studied with an ammoniumsulfate fraction, 30~50%saturation as the enzyme preparation.

Insights into Glycogen Metabolism in Chemolithoautotrophic Bacteria from Distinctive Kinetic and Regulatory Properties of ADP-Glucose Pyrophosphorylase from Nitrosomonas europaea

“…4B. This optimal pH was the same as the one reported for the enzyme from Arthrobacter simplex (29) , results may be related to the distinct electronic characteristics determined for the metals and their particular group coordination preferences (16).…”