Preparation of Adhesion Culture of Neural Stem/Progenitor Cells of the Olfactory Mucosa for the Treatment of Spinal Cord Injuries

Степанова, О. В.; Воронова, А Д; Fursa, G. A.; Karsuntseva, E. K.; Valikhov, Marat P.; Чадин, А. В.; Vishnevskii, D A; Reshetov, Igor V.; Чехонин, В. П.

doi:10.1007/s10517-020-05023-0

Bull Exp Biol Med

2020

DOI: 10.1007/s10517-020-05023-0

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Preparation of Adhesion Culture of Neural Stem/Progenitor Cells of the Olfactory Mucosa for the Treatment of Spinal Cord Injuries

О. В. Степанова

А Д Воронова

G. A. Fursa

et al.

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Cited by 2 publications

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Differentiation of neural stem cells from human olfactory mucosa into dopaminergic neuron‐like cells

Ertem,

Uysal

2024

IUBMB Life

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The aim of this study was to develop an alternative treatment method for neurodegenerative diseases with dopaminergic neuron loss such as Parkinson's disease by differentiating cells obtained from human olfactory mucosa‐derived neural stem cells (hOM‐NSCs) with neurotrophic agents in vitro. hOM‐NSCs were isolated and subjected to immunophenotypic and MTT analyses. These hOM‐NSCs were then cultured in a 3D environment to form neurospheres. The neurospheres were subjected to immunophenotypic analysis and neuronal differentiation assays. Furthermore, hOM‐NSCs were differentiated into dopaminergic neuron‐like cells in vitro. After differentiation, the dopaminergic neuron‐like cells were subjected to immunophenotypic (TH, MAP2) and genotypic (DAT, PITX3, NURR1, TH) characterization. Flow cytometric analysis showed that NSCs were positive for cell surface markers (CD56, CD133). Immunofluorescence analysis showed that NSCs were positive for markers with neuronal and glial cell characteristics (SOX2, NESTIN, TUBB3, GFAP and NG2). Immunofluorescence analysis after differentiation of hOM‐NSCs into dopaminergic neuron‐like cells in vitro showed that they were positive for a protein specific for dopaminergic neurons (TH). qRT‐PCR analysis showed that the expression of dopaminergic neuron‐specific genes (DAT, TH, PITX3, NURR1) was significantly increased. It was concluded that hOM‐NSCs may be a source of neural stem cells that can be used for cell replacement therapies in neurodegenerative diseases such as Parkinson's disease, are resistant to cell culture, can differentiate into neuronal and glial lineage, are easy to obtain and are cost effective.

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Differentiation of neural stem cells from human olfactory mucosa into dopaminergic neuron‐like cells

Ertem,

Uysal

2024

IUBMB Life

View full text Add to dashboard Cite

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Modeling of Alzheimer’s Disease to Study the Efficacy of Cell Therapy (Review)

Voronova,

Karsuntseva,

Stepanova

et al. 2023

Bull Exp Biol Med

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Preparation of Adhesion Culture of Neural Stem/Progenitor Cells of the Olfactory Mucosa for the Treatment of Spinal Cord Injuries

Cited by 2 publications

References 19 publications

Differentiation of neural stem cells from human olfactory mucosa into dopaminergic neuron‐like cells

Differentiation of neural stem cells from human olfactory mucosa into dopaminergic neuron‐like cells

Modeling of Alzheimer’s Disease to Study the Efficacy of Cell Therapy (Review)

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