Preparation of a Stable Mycobacterial Tween Hydrolysis Test Substrate

Of 1,791 tissues submitted from 1975 to 1978 for histological examination for tuberculosis, 78.5% were classified as tuberculosis, 8.1% as club-forming granulomas, 3.3% as hydatid cysts, 3.4% as other granulomas and 2.4% as squamous cell carcinomas. Cultural examination of 2,682 tissues for mycobacteria yielded Mycobacterium bovis from 42.88%, other mycobacteria from 7.64% and 0.89% Rhodococcus (Corynebacterium) equi. Of 1,478 tissues on which both examinations were performed, culture and histology results were in agreement on 94.1%. M. Bovis was isolated on one or more occasions from 564 herds with the highest herd prevalence being recorded in western areas of the state. Culture of 1,024 tissues from no visible lesion (NVL) reactors yielded 0.59% M. bovis, 15.04% other mycobacteria and 0.19% R. equi. Of 120 isolates of other mycobacteria from tissues of NVL reactors, 39 were Runyon Group III (20 M.avium complex) and 61 Runyon Group IV. Approximately half of the herds from which NVL tissues were collected were confirmed M. bovis infected properties.

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Multiple drug resistance in Mycobacterium avium: is the wall architecture responsible for exclusion of antimicrobial agents?

Rastogi¹,

Fréhel²,

Ryter³

et al. 1981

Antimicrob Agents Chemother

196

129

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Whole cells of Mycobacterium avium, characterized by their negative response in the nine biochemical tests used for mycobacterial identification in our laboratory, turned positive for nitrate reductase, Tween-80 hydrolysis, beta-glucosidase, acid phosphatase, alkaline phosphatase, penicillinase, and trehalase after their wall portion was removed to yield spheroplasts. This suggested that the negative results in most of the biochemical procedures were caused by the exclusion mechanism at the wall level. Preliminary transmission and scanning electron microscopic studies showed differences at wall level between laboratory-maintained opaque, dome-shaped (SmD) and host-recycled smooth, transparent (SmT) colony type variants of M. avium and suggested the presence of an outer regularly structured layer in SmT variants. Comparative ultrastructural studies utilizing different polysaccharide coloration methods confirmed the presence of an outer polysaccharide layer in SmT variants which was probably related to their enhanced pathogenicity for experimental animals and drug resistance as compared to that of SmD variants. These findings are discussed with respect to multiple drug resistance, virulence, and gene expression of M. avium.

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Preparation of a Stable Mycobacterial Tween Hydrolysis Test Substrate

Abstract: A Tween 80 hydrolysis test substrate was found to be stable at room temperature for at least 6 months.

Cited by 5 publications

References 2 publications

Biochemical and cultural analysis of mycobacterial recombinants obtained by spheroplast fusion

Biochemical and cultural analysis of mycobacterial recombinants obtained by spheroplast fusion

THE DISTRIBUTION OF MYCOBACTERIUM BOVIS IN QUEENSLAND CATTLE HERDS WITH OBSERVATIONS ON THE LABORATORY DIAGNOSIS OF TUBERCULOSIS

Multiple drug resistance in Mycobacterium avium: is the wall architecture responsible for exclusion of antimicrobial agents?

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