Preparation of a silicate-containing hydroxyapatite-based coating by magnetron sputtering: structure and osteoblast-like MG63 cells in vitro study

Abstract

Silicate-containing hydroxyapatite-based coatings with different structure and calcium/phosphate ratios were prepared by radio-frequency magnetron sputtering on silicon and titanium substrates, respectively. Scanning electron microscopy, X-ray diffraction and IR spectroscopy were used to investigate the effect of the substrate bias on the properties of the silicate-containing hydroxyapatite-based coatings. The deposition rate, composition, and microstructure of the deposited coatings were all controlled by changing the bias voltage from grounded (0 V) to 250 and 2100 V. The biocompatibility was assessed by cell culture with human osteoblast-like cells (MG-63 cell line), showing a good biocompatibility and cell growth on the substrates.

Bioceramic Coatings for Metallic Implants

“…An extensive range of sputter deposition parameters have been investigated and shown to influence the properties of the resultant HA-based surfaces, including discharge power level [105], gas pressure [106], thermal processing conditions [107], and target stoichiometry [99,100,108]. For a given operational condition, the target composition and stoichiometry can directly influence the Ca/P ratio of the sputter-deposited surface [104].…”

“…This segregation results in the formation of the amorphous phase in the HA films. The influence on the crystallinity and biological properties of the magnetron sputterdeposited films due to the silicate doping of the CaP coating was examined by the authors [91,99]. The partial dissolution of the CaP ceramic initiates the re-precipitation of the biological apatite crystals and subsequent protein and cell attachment to the implant surface [81].…”

“…A novel Si-doped HA-based coating, in which the silicate ions are substituted by phosphorous ions, was fabricated [99][100][101]. Moreover, the precursor powder of Si-HA (Ca 10 (PO 4 ) 6-x (SiO 4 ) x (OH) 2-x , where x = 0.5 and 1.72) or Ag-HA (Ca 10Àx Ag x (PO 4 ) 6 (OH) 2Àx , where x = 0.3 and 1.5) was used for the deposition of the Si-or Ag-doped HA films [102].…”

Anti-bacterial surfaces: natural agents, mechanisms of action, and plasma surface modification

“…[136][137][138] Indeed, over the last 20 years, plasma-enabled nanoscale synthesis and modification have evolved from a relatively simple tool for materials science and microelectronics into a highly sophisticated instrument for development of a wide range of pure and hybrid nanoscale objects spanning across a vast number of materials systems and length scales ( Figure 8). 139 At the present level of development, low-temperature plasmas afford chemists and material scientists the level of confidence comparable to, and in many cases superior to, conventional processing techniques, e.g.…”

Effect of pulsed electron beam treatment on the physico-mechanical properties of hydroxyapatite-coated titanium

“…Among many other deposition techniques, radiofrequency (RF) magnetron sputtering was used to coat titanium substrates with HA [3,4]. RFmagnetron sputtering is a well-known method to produce pure HA coatings [5,6], as well as HA coatings with different admixtures, such as Si [7] or Ag [8]. The ability to prepare thin coatings with a strong adhesion and compact microstructure is a significant advantage of this technique.…”