Preparation of a pure inoculum of acetic acid bacteria for the selective conversion of glucose in strawberry purée into gluconic acid

Cañete-Rodríguez, Ana M.; Santos-Dueñas, Inés M.; Torija, María-Jesús; Mas, Albert; Jiménez-Hornero, Jorge E.; García-García, Isidoro

doi:10.1016/j.fbp.2015.06.005

Cited by 18 publications

(6 citation statements)

References 29 publications

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“…We have developed a strawberry beverage in which D -glucose is completely fermented to GA or some other acids, yet fruit fructose is maintained as a natural sweetener ( Cañete-Rodríguez et al, 2015 , 2016 ). GA could be found naturally in fruit juices, honey, yogurt, bread, cottage cheese and meat.…”

Section: Introductionmentioning

confidence: 99%

Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains

et al. 2016

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Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter were phylogenetically more closely related each other than with Gluconobacter.

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Section: Introductionmentioning

confidence: 99%

Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains

et al. 2016

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“…Our interest in these strains was for their use in the production of gluconate from d -glucose without the oxidation of fructose ( 15 ). This transformation is sought for use in the production of new strawberry beverages based on the presence of fructose as a sweetener and free of glucose because it is converted into gluconic acid or the corresponding gluconates ( 16 – 18 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Gluconobacter cerinus CECT 9110 and Gluconobacter japonicus CECT 8443, Acetic Acid Bacteria Isolated from Grape Must

2016

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“…Regarding new strategies for G. oxydans bioprocesses, the search for cheaper feedstocks such as residual biomass or industrial byproducts, have been increased lately. Agroindustrial wastes (sugars from hydrolysis) and biodiesel byproducts (crude glycerol), have already been reported for gluconic acid (13) (Cañete-Rodríguez et al 2015;Zhang et al 2016a;Hou et al 2018;Zhou and Xu 2019) and dihydroxyacetone (18) synthesis, respectively (Dikshit and Moholkar 2016a;Poljungreed and Boonyarattanakalin 2017;Stasiak-Rózańska et al 2018). The use of residual feedstocks not only can reduce costs, but could also be a solution for waste disposal.…”

Section: Introductionmentioning

confidence: 99%

The industrial versatility of Gluconobacter oxydans: current applications and future perspectives

Alves-Ribeiro

Oliveira

Lima

et al. 2022

World J Microbiol Biotechnol

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Gluconobacter oxydans is a well-known acetic acid bacterium that has long been applied in the biotechnological industry. Its extraordinary capacity to oxidize a variety of sugars, polyols, and alcohols into acids, aldehydes, and ketones is advantageous for the production of valuable compounds. Relevant G. oxydans industrial applications are in the manufacture of L-ascorbic acid (vitamin C), miglitol, gluconic acid and its derivatives, and dihydroxyacetone. Increasing efforts on improving these processes have been made in the last few years, especially by applying metabolic engineering. Thereby, a series of genes have been targeted to construct powerful recombinant strains to be used in optimized fermentation. Furthermore, low-cost feedstocks, mostly agro-industrial wastes or byproducts, have been investigated, to reduce processing costs and improve the sustainability of G. oxydans bioprocess. Nonetheless, further research is required mainly to make these raw materials feasible at the industrial scale. The current shortage of suitable genetic tools for metabolic engineering modifications in G. oxydans is another challenge to be overcome. This paper aims to give an overview of the most relevant industrial G. oxydans processes and the current strategies developed for their improvement.

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Preparation of a pure inoculum of acetic acid bacteria for the selective conversion of glucose in strawberry purée into gluconic acid

Cited by 18 publications

References 29 publications

Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains

Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains

Draft Genome Sequences of Gluconobacter cerinus CECT 9110 and Gluconobacter japonicus CECT 8443, Acetic Acid Bacteria Isolated from Grape Must

The industrial versatility of Gluconobacter oxydans: current applications and future perspectives

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