Preparation of a pure autologous biodegradable fibrin matrix for tissue engineering

Abstract

Parallel to the growing role of tissue engineering, the need for cell embedding materials, which allow cells to stabilise in a three-dimensional distribution, has increased. Although several substances have been tested, fibrin is thus far the only one that permits the clinical application of cultured tissue. To date, autologous fibrinogen has usually been polymerised with bovine thrombin, which can cause severe immunological side effects. The objective of this study was to explore the practicability of obtaining autologous thrombin from a single patient in an adequate concentration and amount. Fibrinogen was cryoprecipitated from 200 ml of freshly-frozen plasma. Thrombin was isolated from the supernatant through ion-exchange chromatography. The thrombin was first bound to Sephadex A-50 and then eluated using 2 ml of a salt buffer (2.0 M NaCl in 0.015 M trisodiumcitrate, pH 7.0). The activity of the thrombin (51 NIH x ml(-1) to 414 NIH x ml(-1) reached levels comparable to those in commercially available fibrin glues (4-500 NIH x ml(-1)). The study has shown that it is possible to obtain a sufficient amount of autologous thrombin from a single donor to create a fibrin matrix of high efficiency without the risk of immunological and infectious side effects.

Genipin Cross-Linked Fibrin Hydrogels for in vitro Human Articular Cartilage Tissue-Engineered Regeneration

“…Nevertheless, we have shown that the human fibrin gel system offers a potential patient-specific autologous scaffold for cartilage regeneration, since human fibrinogen and thrombin can be isolated separately from a patient's fresh plasma, and encapsulated cells can be donated by the same patient [Rock et al, 2001]. The advantage of a completely autologous approach for gel formation would be the elimination of the risk of tissue incompatibility and viral infection [Haisch et al, 2000] as well as an associated ease of transition to clinical use.…”

The Evolution of Vascular Tissue Engineering and Current State of the Art

“…While most studies have been performed with isolated/processed components, a possible advantage of using fibrin is that fibrinogen and thrombin, the precursors to fibrin gel formation, can be readily obtained from a patient's own blood [Haisch et al, 2000]. Like collagen gel-based grafts, constructs created from fibrin-gels have a typically low mechanical strength.…”

Modeling and simulation of material degradation in biodegradable wound closure devices

“…The healing time for wound varies among different kinds of tissues, 12 and the biodegradable devices are required to maintain stability until wound heals. 13 Degradation/corrosion mechanism of the biodegradable materials which can be used as reference for biodegradable device design has received a lot of attention from the scientific community. There were many studies on the corrosion mechanism of Mg alloy as a biodegradable material.…”