Preparation of a protein‐free total brain white matter lipid fraction: Characterization of liposomes

Díaz, R. S.; Monreal, J.; Regueiro, Pilar; Lucas, Marguerite

doi:10.1002/jnr.490310119

Cited by 14 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This polar ether-based 3DISCO protocol has achieved extremely rapid dehydration due to its high permeable kinetics and has provided fully cleared organ samples after RI matching. Because THF has significant lipophilicity (Díaz et al 1992), this protocol may accompany highly effective delipidation. THF-solvated tissues are more slightly shrunk than EtOH-solvated ones, probably because THF lacks donating hydrogen.…”

Section: Water-miscible Polar Solventsmentioning

confidence: 99%

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

Tainaka

Kuno

Kubota

et al. 2016

Annu. Rev. Cell Dev. Biol.

231

239

View full text Add to dashboard Cite

Mammalian bodies have more than a billion cells per cubic centimeter, which makes whole-body cell (WBC) profiling of an organism one of the ultimate challenges in biology and medicine. Recent advances in tissue-clearing technology have enabled rapid and comprehensive cellular analyses in whole organs and in the whole body by a combination of state-of-the-art technologies of optical imaging and image informatics. In this review, we focus mainly on the chemical principles in currently available techniques for tissue clearing and staining to facilitate our understanding of their underlying mechanisms. Tissue clearing is usually conducted by the following steps: (a) fixation, (b) permeabilization, (c) decolorizing, and (d) refractive index (RI) matching. To phenotype individual cells after tissue clearing, it is important to visualize genetically encoded fluorescent reporters and/or to stain tissues with fluorescent dyes, fluorescent labeled antibodies, or nucleic acid probes. Although some technical challenges remain, the chemical principles in tissue clearing and staining for WBC profiling will enable various applications, such as identifying cellular circuits across multiple organs and measuring their dynamics in stochastic and proliferative cellular processes, for example, autoimmune and malignant neoplastic diseases.

show abstract

Section: Water-miscible Polar Solventsmentioning

confidence: 99%

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

Tainaka

Kuno

Kubota

et al. 2016

Annu. Rev. Cell Dev. Biol.

231

239

View full text Add to dashboard Cite

show abstract

“…We examined two more complicated model systems: total brain extract lipids (TBE-lipids) and a ternary system consisting of DOPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol at a 1:2:1 ratio. TBE-lipids have been widely used as models of the plasma membrane and have been used in studies of IAPP-membrane interactions (28,(39)(40)(41). They are considerably more complex than standard one-or two-component model membrane systems.…”

Section: Folded Monomeric Proteins Are Less Effective At Inducing Modelmentioning

confidence: 99%

Islet amyloid polypeptide toxicity and membrane interactions

Cao

Abedini

Wang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

130

143

View full text Add to dashboard Cite

Islet amyloid polypeptide (IAPP) is responsible for amyloid formation in type 2 diabetes and contributes to the failure of islet cell transplants, however the mechanisms of IAPP-induced cytotoxicity are not known. Interactions with model anionic membranes are known to catalyze IAPP amyloid formation in vitro. Human IAPP damages anionic membranes, promoting vesicle leakage, but the features that control IAPP-membrane interactions and the connection with cellular toxicity are not clear. Kinetic studies with wildtype IAPP and IAPP mutants demonstrate that membrane leakage is induced by prefibrillar IAPP species and continues over the course of amyloid formation, correlating additional membrane disruption with fibril growth. Analyses of a set of designed mutants reveal that membrane leakage does not require the formation of β-sheet or α-helical structures. A His-18 to Arg substitution enhances leakage, whereas replacement of all of the aromatic residues via a triple leucine mutant has no effect. Biophysical measurements in conjunction with cytotoxicity studies show that nonamyloidogenic rat IAPP is as effective as human IAPP at disrupting standard anionic model membranes under conditions where rat IAPP does not induce cellular toxicity. Similar results are obtained with more complex model membranes, including ternary systems that contain cholesterol and are capable of forming lipid rafts. A designed point mutant, I26P-IAPP; a designed double mutant, G24P, I26P-IAPP; a double N-methylated variant; and pramlintide, a US Food and Drug Administration-approved IAPP variant all induce membrane leakage, but are not cytotoxic, showing that there is no one-to-one relationship between disruption of model membranes and induction of cellular toxicity.

show abstract

“…1) characterized by an EY value of 94% and an EC value of 14 l/ mol. The EC value was much higher than that expected for unilamellar liposomes [26]. This finding may be due to two different factors: i) the precipitation of the drug molecule as sulfate salt (used for the generation of the acid pH gradient) in aqueous compartments of unilamellar liposomes and/or ii) the interaction of gemcitabine with the molecules of phospholipids [27].…”

Section: Cell From Viabilitymentioning

confidence: 77%

In Vitro Evaluation of the Activity of Gemcitabine-Loaded Pegylated Unilamellar Liposomes Against Papillary Thyroid Cancer Cells~!2010-04-18~!2010-06-27~!2010-08-23~!

Vono¹,

Cosco²,

Celia³

et al. 2010

TODDJ

View full text Add to dashboard Cite

Papillary carcinoma is the most common form of malignant thyroid tumor. At present, a subset of these tumors are poorly responsive to the current treatment. Gemcitabine is a pyrimidine analog active against different types of solid tumors, but its use is limited by its short half-life. To improve the therapeutic effectiveness of this drug, gemcitabineloaded unilamellar pegylated liposomes were prepared and investigated against two human papillary thyroid carcinoma cell lines, i.e. TPC-1 and B-CPAP cells. The pH gradient drug encapsulation followed by the membrane extrusion technique were used to achieve unilamellar liposomes characterized by a mean size of ~200 nm, a polydispersity index of 0.02 and a zeta potential of-1.7 mV. The gemcitabine was released from liposomes following a biphasic profile. The liposomal encapsulated gemcitabine showed an increased cytotoxic activity compared to the free drug against both thyroid carcinoma cell lines, as a consequence of the better drug internalization favored by the vesicular device. These findings demonstrate the advantage of channeling gemcitabine by liposomes suggesting a promising role for such a pharmaceutical formulation in the treatment of refractory papillary thyroid carcinoma.

show abstract

Preparation of a protein‐free total brain white matter lipid fraction: Characterization of liposomes

Cited by 14 publications

References 37 publications

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

Chemical Principles in Tissue Clearing and Staining Protocols for Whole-Body Cell Profiling

Islet amyloid polypeptide toxicity and membrane interactions

In Vitro Evaluation of the Activity of Gemcitabine-Loaded Pegylated Unilamellar Liposomes Against Papillary Thyroid Cancer Cells~!2010-04-18~!2010-06-27~!2010-08-23~!

Contact Info

Product

Resources

About