Preparation of a pressurization system to study the effect of hydrostatic pressure on chondrocyte cultures

Nerucci, F; Fioravanti, Antonella; Cicero, Maria Rosaria; Marcolongo, Roberto; Spinelli, Giuseppe

doi:10.1007/s11626-998-0041-x

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…Our pressurization system presents some special characteristics already described in detail [ 56 ]. In the present study the chondrocytes cultivated on Petri dishes were exposed to cyclic pressurization by sinusoidal waves (minimum pressure 1MPa and maximum pressure 5 MPa) at 0.25 Hz frequency for a 3 h period.…”

Section: Methodsmentioning

confidence: 99%

Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure

Pascarelli

Collodel

Moretti

et al. 2015

IJMS

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The aim of this study was to examine the ultrastructure and cytoskeletal organization in human normal and Osteoarhritic (OA) chondrocytes, exposed to interleukin-1β (IL-1β) and cyclic hydrostatic pressure (HP). Morphological examination by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed differences between normal and OA chondrocytes at the nuclear and cytoplasmic level. IL-1β (5 ng/mL) induced a decrease of the number of mitochondria and Golgi bodies and a significant increase on the percentage of cells rich in vacuolization and in marginated chromatin. Cyclical HP (1–5 MPa, 0.25 Hz, for 3 h) did not change the morphology of normal chondrocytes, but had a beneficial effect on OA chondrocytes increasing the number of organelles. Normal and OA cells subjected to IL-1β and HP recovered cytoplasmic ultrastructure. Immunofluorescence (IF) examination of normal chondrocytes showed an actin signal polarized on the apical sides of the cytoplasm, tubulin and vimentin uniformly distributed throughout cytoplasm and vinculin revealed a punctuated pattern under the plasma membrane. In OA chondrocytes, these proteins partially lost their organization. Stimulation with IL-1β caused, in both type of cells, modification in the cytoskeletal organization; HP counteracted the negative effects of IL-1β. Our results showed structural differences at nuclear, cytoplasmic and cytoskeletal level between normal and OA chondrocytes. IL-1β induced ultrastructural and cytoskeletal modifications, counteracted by a cyclical low HP.

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Section: Methodsmentioning

confidence: 99%

Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure

Pascarelli

Collodel

Moretti

et al. 2015

IJMS

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“…Our pressurization system has some special characteristics that have already been described in detail 32 . Briefly, this pressure chamber system consists of a hermetically sealed stainless steel cylinder, 400 mm high with an internal diameter of 90 mm.…”

Section: Pressurization Systemmentioning

confidence: 99%

Effect of hyaluronic acid (MW 500–730kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed to hydrostatic pressure

Fioravanti

Cantarini

Chellini

et al. 2005

Osteoarthritis and Cartilage

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Hydrostatic pressure as epigenetic modulator in chondrocyte cultures: A study on miRNA-155, miRNA-181a and miRNA-223 expression levels

Palma

Cheleschi

Pascarelli

et al. 2018

Journal of Biomechanics

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Preparation of a pressurization system to study the effect of hydrostatic pressure on chondrocyte cultures

Cited by 5 publications

References 9 publications

Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure

Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure

Effect of hyaluronic acid (MW 500–730kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed to hydrostatic pressure

Hydrostatic pressure as epigenetic modulator in chondrocyte cultures: A study on miRNA-155, miRNA-181a and miRNA-223 expression levels

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