Preparation of a population of macrophages from human third trimester decidua

Adeleye, T. A.; Elder, M. G.; Sullivan, M.

doi:10.1093/humrep/11.2.451

Cited by 3 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This latter finding highlights the complexity of the decidua and its specialized biology, which to date has not been exploited for therapeutic intervention. Several studies have described methods that yield highly purified decidual cell preparations [17][18][19] and have been utilized to investigate immune [17,20,21] and endocrine [18] aspects of decidual biology but, hitherto, not their electrophysiology.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Potassium Currents in Human Decidua Before and After the Onset of Labor1

Liu¹,

Arulkumaran²,

Hill³

et al. 2003

Biology of Reproduction

View full text Add to dashboard Cite

The human decidua plays a prominent role in the signaling between maternal and fetal tissues. It also secretes a plethora of molecules that modulate uterine function. Ion-channel activity underpins many cellular functions; however, the channels in human decidua have not been characterized in any detail. We have used the whole-cell recording mode of the patch-clamp technique to carry out current-clamp and voltage-clamp recordings of membrane properties and whole-cell potassium (K ؉ ) currents of freshly isolated decidual stromal cells. Decidual tissue was obtained from women after spontaneous vaginal delivery (SVD) or elective cesarean section (CS). Cells from both groups generated action potentials, the overshoots and durations of which were dependent on extracellular calcium levels, inhibited by cobalt and enhanced by barium. Potassium current (I K ) density was higher in the CS than in the SVD group. Outwardly directed currents were heterogeneous with respect to their activation/inactivation profiles and exhibited outward rectification. The main difference between the SVD and CS group was the presence of a sustained current component in CS cells that is tetraethylammonium chloride-resistant and appears to be unaffected by E-4031. No evidence for the activation of any calcium-activated K ؉ currents was obtained. We propose that human parturition is associated with subtle changes in K ؉ channel remodeling, reflecting the transition from uterine quiescence to activation and stimulation. An understanding of the signal transduction events underlying these process may eventually lead to novel approaches to prevent preterm labor via decidual rather than myometrial intervention.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Potassium Currents in Human Decidua Before and After the Onset of Labor1

Liu¹,

Arulkumaran²,

Hill³

et al. 2003

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…This may explain why decidua explants secrete slightly less secretory leukocyte protease inhibitor compared with chorio-decidua, unless there is a gradient of secretion with highest outputs from decidua adjacent to the chorion. Term decidua is heterogeneous, consisting of stromal cells (53%), macrophages (19%), granulocytes (16%) and T-cells (8%) (Adeleye et al 1996). Hence the stromal cells, macrophages and granulocytes, which are capable of secretory leukocyte protease inhibitor secretion in other sites (Ohlsson et al 1996), are potential sources.…”

Section: Discussionmentioning

confidence: 99%

Secretory leukocyte protease inhibitor concentration increases in amniotic fluid with the onset of labour in women: characterization of sites of release within the uterus

Denison¹,

Kelly²,

Calder³

et al. 1999

Journal of Endocrinology

View full text Add to dashboard Cite

Secretory leukocyte protease inhibitor is a potent inhibitor of neutrophil function, a mediator of mucosal immunity and an inhibitor of NF B regulated inflammatory responses. However, its source, function and regulation within the uterus during pregnancy and at parturition are not well defined. In amniotic fluid, the concentration of secretory leukocyte protease inhibitor increased significantly from 2nd trimester (24 3 ng/ml; mean ...; n=20) to term (751 53 ng/ml; P<0·05; n=15) with a further profound increase (P<0·005) with the onset of labour (3929 1076 ng/ml; n=15). To establish the intrauterine sites of secretion, explants (n=6 different patients per tissue) were collected at term after elective caesarean section. High levels of secretory leukocyte protease inhibitor were released by decidua (135·2 12·4 pg/mg; mean ...) and chorio-decidua (325·1 26·4 pg/mg) with less by amnion (55·6 6·0 pg/mg) and placenta (9·2 1·9 pg/mg). Intense immunoreactivity for secretory leukocyte protease inhibitor was detected predominantly in decidua parietalis cells adherent to the chorion laeve and myometrium, and also in decidua basalis. We propose that, within the pregnant uterus, secretory leukocyte protease inhibitor is released by decidua, fetal membranes and potentially the fetal lung. The increase in secretory leukocyte protease inhibitor may act to modulate proinflammatory paracrine interactions for the maintenance of pregnancy and limit those occurring at parturition within the uterus.

show abstract

“…Macrophages within the uteroplacental environment are an important source of bioactive mediators including prostaglandins and cytokines. Placental and decidual macrophages express COX-2 and produce PGE 2 in response to LPS or the pro-inflammatory cytokine IL-1β [ 25 – 29 ]. No studies to date have examined the effects of environmental toxicants, such as MEHP, on inducible COX-2 expression or prostaglandin secretion in macrophages from the utero-placental unit.…”

Section: Introductionmentioning

confidence: 99%

Mono-ethylhexyl phthalate stimulates prostaglandin secretion in human placental macrophages and THP-1 cells

Tetz

Aronoff

Loch‐Caruso

2015

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundDiethylhexyl phthalate (DEHP) is widely used as a plasticizer in polyvinyl chloride products. DEHP exposure, which is widespread in the US, increases preterm birth risk; however, the mechanisms driving this relationship are unclear. Because cyclooxygenase-2 (COX-2) dependent prostaglandin synthesis is implicated in preterm birth, we evaluated effects of mono-2-ethylhexyl phthalate (MEHP), the active metabolite of DEHP, on prostaglandin E2 (PGE2) synthesis and COX expression in human placental macrophages (PM). In addition, responses in PM were compared to those in a human macrophage-like cell line, THP-1.MethodsPM and THP-1 cells were treated for 2, 4, 8, or 24 h with MEHP concentrations ranging from 10 to 180 micromolar. PGE2 concentrations were assessed in culture medium using ELISA, and COX expression was determined by western blot.ResultsTreatment of PM and THP-1 cells with 180 micromolar MEHP for 24 h significantly increased PGE2 release. Co-treatment of PMs or THP-1 cells with 180 micromolar MEHP and the non-selective COX inhibitor indomethacin reduced MEHP-stimulated PGE2 production. Similarly, co-treatment of PM and THP-1 cells with the COX-2 selective inhibitor NS-398 resulted in a significant decrease in PGE2, suggesting that MEHP-stimulated PGE2 is dependent specifically on increased COX-2 expression. Western blot analysis revealed a significant increase in COX-2 expression in PM and THP-1 cells treated with 180 micromolar MEHP, and no changes in COX-1 expression, supporting the role of COX-2 in MEHP-stimulated PGE2 synthesis.ConclusionsThe findings from this study are the first to demonstrate phthalate-stimulated PGE2 synthesis in PM and warrant future studies into COX-2-dependent prostaglandin synthesis as a mechanism of toxicant-associated preterm birth.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0046-8) contains supplementary material, which is available to authorized users.

show abstract

Preparation of a population of macrophages from human third trimester decidua

Cited by 3 publications

References 22 publications

Comparison of Potassium Currents in Human Decidua Before and After the Onset of Labor1

Comparison of Potassium Currents in Human Decidua Before and After the Onset of Labor1

Secretory leukocyte protease inhibitor concentration increases in amniotic fluid with the onset of labour in women: characterization of sites of release within the uterus

Mono-ethylhexyl phthalate stimulates prostaglandin secretion in human placental macrophages and THP-1 cells

Contact Info

Product

Resources

About