Preparation of a polyclonal antibody that recognizes a unique galactoseβ1-4fucose disaccharide epitope

Takeuchi, Tomoharu; Nishiyama, Kazusa; Saito, Saori; Tamura, Masahito; Fuwa, Takashi J.; Nishihara, Shoko; Takahashi, Hideyo; Natsugari, Hideaki; Arata, Yoichiro; Kasai, Ken Ichi

doi:10.1016/j.carres.2015.04.015

Cited by 4 publications

(3 citation statements)

References 39 publications

(32 reference statements)

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“…The occurrence of the Galβ1‐4Fuc unit has also been reported in other Protostomia such as the planarian Dugesia japonica . Additionally, we found anti‐Galβ1‐4Fuc antibody‐reactive substances in the allergenic mite Dermatophagoides pteronyssinus belonging to Arthropoda .…”

Section: Occurrence Of N‐glycans Containing the Galβ1‐4fuc Unitsupporting

confidence: 84%

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Galactoseβ1‐4fucose: A unique disaccharide unit found in N‐glycans of invertebrates including nematodes

2016

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Galactoseβ1-4fucose (Galβ1-4Fuc), a unique disaccharide unit found only on the N-glycans of Protostomia, has been intensively studied, particularly in Nematoda. Galβ1-4Fuc attached to the 6-OH of the innermost GlcNAc of N-glycans has been identified as an endogenous target recognized by Caenorhabditis elegans galectin LEC-6 and might function as an endogenous ligand for other galectins as well. Interactions between galectins and N-glycans might be subject to fine-tuning through modifications of the penultimate GlcNAc and the Galβ1-4Fuc unit. Similar fine-tuning is also observable in vertebrate galectins, although their major recognition unit is a Galβ1-4GlcNAc. In Protostomia, it can be postulated that glycan-binding proteins and their ligands have coevolved; however, epitopes such as Galβ1-4Fuc were then hijacked as targets by other organisms. Fungal (Coprinopsis cinerea) galectin 2, CGL2, binds the Galβ1-4Fuc on C. elegans glycans to exert its nematotoxicity. Some human and mouse galectins bind to synthesized Galβ1-4Fuc; as some parasitic nematodes express this motif, its recognition by mammalian galectins could hypothetically be involved in host defense, similar to fungal CGL2. In this review, we discuss the Galβ1-4Fuc unit in Protostomia as a possible equivalent for the Galβ1-4GlcNAc unit in vertebrates and a potential non-self glycomarker useful for pathogen recognition.

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Section: Occurrence Of N‐glycans Containing the Galβ1‐4fuc Unitsupporting

confidence: 84%

“…Although D. melanogaster has at least one potential GALT-1 homolog, glycome analyses of insects failed to detect any Gal␤1-4Fuc structure [34,[44][45][46][47]. Western blotting using anti-Gal␤1-4Fuc antibodies also yielded negative results [41].…”

Section: Biosynthesis Of the Gal␤1-4fuc Structurementioning

confidence: 99%

Galactoseβ1‐4fucose: A unique disaccharide unit found in N‐glycans of invertebrates including nematodes

2016

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“…It may be worth noting that IgE-binding carbohydrate determinants could be implicated in cross-reactivity between helminth and HDM; in fact, a galactose β1-4fucose epitope has been detected in N-glycans shared by A. suum and D. pteronyssinus. 80 We have found several glycosylated components of high molecular weight (>100 kDa) in A. lumbricoides extract that were inhibited by preabsorption of sera with HDM extracts; however, deglycosilation does not significantly affect the specific IgE antibody binding to the extract. 29 Cross-reactivity due to carbohydrate determinants may affect specific IgE measurements in vitro.…”

Section: Cross-reactivity Between Hdm and Ascaris Componentsmentioning

confidence: 86%

Ascariasis as a model to study the helminth/allergy relationships

Caraballo

Acevedo

Zakzuk

2018

Parasite Immunology

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Summary Ascariasis is the most frequent soil transmitted helminthiasis and, as well as other helminth infections, is expected to influence the clinical presentation of allergic diseases such as asthma. Indeed, several clinical and experimental works have shown an important impact either increasing or suppressing symptoms, and the same effects have been detected on the underlying immune responses. In this review we analyze the work on this field performed in Colombia, a Latin American tropical country, including aspects such as the molecular genetics of the IgE response to Ascaris; the allergenic activity of Ascaris IgE‐binding molecular components and the immunological and clinical influences of ascariasis on asthma. The analysis allows us to conclude that the impact of ascariasis on the inception and evolution of allergic diseases such as asthma deserves more investigation, but advances have been made during the last years. The concurrent parasite‐induced immunostimulatory and immunosuppressive effects during this helminthiasis do modify the natural history of asthma and some aspects of the practice of allergology in the tropics. Theoretically it can also influence the epidemiological trends of allergic diseases either by its absence or presence in different regions and countries.

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Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method

Fei

Dong

et al. 2018

Virus Research

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Preparation of a polyclonal antibody that recognizes a unique galactoseβ1-4fucose disaccharide epitope

Cited by 4 publications

References 39 publications

Galactoseβ1‐4fucose: A unique disaccharide unit found in N‐glycans of invertebrates including nematodes

Galactoseβ1‐4fucose: A unique disaccharide unit found in N‐glycans of invertebrates including nematodes

Ascariasis as a model to study the helminth/allergy relationships

Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method

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