Preparation of a novel carboxyl stationary phase by “thiol‐ene” click chemistry for hydrophilic interaction chromatography

Abstract

A novel carboxyl-bonded silica stationary phase was prepared by "thiol-ene" click chemistry. The resultant Thiol-Click-COOH phase was evaluated under hydrophilic interaction liquid chromatography (HILIC) mobile phase conditions. A comparison of the chromatographic performance of Thiol-Click-COOH and pure silica columns was performed according to the retention behaviors of analytes and the charged state of the stationary phases. The results indicated that the newly developed Thiol-Click-COOH column has a higher surface charge and stronger hydrophilicity than the pure silica column. Furthermore, the chromatographic behaviors of five nucleosides on the Thiol-Click-COOH phase were investigated in detail. Finally, a good separation of 13 nucleosides and bases, and four water-soluble vitamins was achieved.

Theory and Practice of UHPLC and UHPLC–MS

“…The material, which is more hydrophilic than pure silica columns, was reported to provide selective separations of 13 nucleosides and bases, and four water soluble vitamins, because of a combined HILICeion exchange mechanism (Lowe, 2010;Peng et al, 2013b).…”

A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography

“…Since the term hydrophilic interaction chromatography (HILIC) was coined in 1990 by Alpert [1], it has been an established chromatographic technique for separation of highly polar analytes that have poor retention on common reverse phase liquid chromatography (RPLC) [2][3][4][5][6][7]. Along with increasing popularity of HILIC, the development of novel HILIC stationary phases (SPs) has received much attention [2][3][4][5][6].…”

Development of Ultra-Performance Liquid Chromatography Method Using Hydrophilic Interaction Liquid Chromatography for Quantification of Azetidine-2-Carboxylic Acid in Rhizomes of Polygonatum sibiricum F. Delaroche

“…[16] The void in polar compound separation has been filled by the recent resurgence of interest in the HILIC technique, which makes use of polar stationary phases in combination with aqueous eluents, and allows the retention of polar analytes including peptides, amino acids, carbohydrates, nucleobases, and nucleosides as well as many other biologically important compounds. [17][18][19][20][21] Chemically, P. sibiricum contains a significant proportion of highly hydrophilic compounds, such as amino acids like ACA.…”