Preparation of a low bleeding polar stationary phase for hydrophilic interaction liquid chromatography

Qian, Kun; Peng, Yahui; Zhang, Feifang; Yang, Bingcheng; Liang, Xinmao

doi:10.1016/j.talanta.2018.01.009

Cited by 21 publications

(13 citation statements)

References 15 publications

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“…The resulting hydrolytically stable stationary phase carries both amide and hydroxyl groups on the polymer surface, which enhance both polarity and stability of the stationary phase. Cross‐linked polyvinylalcohol (PVA) is synthesized by attaching PVA polymers on the silica functionalized with benzylthioethyl groups and has shown to have low bleeding (Qian, Peng, et al, 2018; Qian, Yang, et al, 2018). Polyvinylpyrrolidone is immobilized on silica particles and has been used as the stationary phase in both RPLC and HILIC modes (Zhao et al, 2020).…”

Section: Polar Stationary Phases For Hilicmentioning

confidence: 99%

A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

Guo

2022

Biomedical Chromatography

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Various polar stationary phases are available for hydrophilic interaction chromatography (HILIC) and help drive continuous applications in biomedical, environmental, and pharmaceutical areas in the past decade. Although the stationary phases for HILIC have been reviewed previously, it is an appropriate time to take another look at the progresses made during the past 5 years. The current review provides an overview of the polar stationary phases commercially available for HILIC applications in an effort to assist scientists in selecting suitable columns. New types of stationary phases that were published in literature in the past 5 years are summarized and discussed. The trend in stationary phase research and development is also highlighted. Of particular interest is the experimental evidence for direct interactions of polar analytes with the ligands of the stationary phases under HILIC conditions. In addition, two different approaches have been developed to delineate the relative significance of the partitioning and adsorption mechanisms in HILIC, representing an important advancement in our understanding of the retention mechanisms in HILIC.

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Section: Polar Stationary Phases For Hilicmentioning

confidence: 99%

A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

Guo

2022

Biomedical Chromatography

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“…In addition, this phase was superior to silica-based HILIC phases in terms of pH tolerance (2.1–12.7) and bleeding. Qian et al also suggested a valuable solution to reduce the column bleeding that results from silica dissolution. For this purpose, multiple layers of a poly(vinyl alcohol) cross-linked with glutaraldehyde were coated onto silica gel, leading to a 20-fold reduction in bleeding compared to bare silica.…”

Section: Hilic For Polar and Ionizable Compoundsmentioning

confidence: 99%

Recent Advances in Chromatography for Pharmaceutical Analysis

et al. 2018

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“…As observed with an ELSD, if the detector background is set at zero with ultrafiltered evaporating gas, 50:50 acetonitrile (ACN)/water (highest purity available to us) flowing through 0. units (ALSU), to ∼0.2 ALSU when passed through a C18silica packed column, and to ∼2.35 ALSU when passed through a bare silica packed column (both 4.6 × 150 mm, all at flow rates of 1 mL/min). 10 Compared to the C18-silica used (which may not have been the best protected material), this indicates the dissolution rate for bare silica to be at least an order of magnitude higher. To improve the chromatographic performance of bare silica as an HILIC phase by reducing access to exposed silanol groups, we coated silica beads with one or more layers of poly(vinyl alcohol) (PVA), referred to below as PVA-Sil, with or without further additives and reported on their chromatographic performance.…”

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confidence: 95%

“…22,23 In our experiments, PVA coating of silica dramatically improved chromatographic performance but did not reduce bleeding over bare silica. 10 Water can reach the surface of the silica through the water-permeable PVA; the dissolved silica can then permeate out. We had also coated porous graphitic carbon (PGC) phases with PVA.…”

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confidence: 99%

“…Next, we cross-linked the PVA with boric acid or glutaraldehyde (GA) to reduce water permeability. 10 The GAcross-linked PVA on silica (PVA-GA-Sil) showed much less bleeding. Indeed, among several commercial columns tested, only Acclaim HILIC-10 (this phase contains proprietary polar groups covalently bonded to silica by hydrolysis resistant linkages 24 ) showed marginally less bleeding than silica coated with three layers of GA-cross-linked PVA.…”

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confidence: 99%

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Low-Bleed Silica-Based Stationary Phase for Hydrophilic Interaction Liquid Chromatography

et al. 2018

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Bleeding of hydrophilic interaction liquid chromatographic (HILIC) phases is a major problem. A hydrophobic silica surface exhibits low bleeding but does not behave as a HILIC phase. A hydrophilic coating, such as that of poly(vinyl alcohol) (PVA) on a hydrophobic particle should result in a low-bleed hydrophilic phase. However, a silica particle functionalized in a hydrophobic manner is not wetted by PVA and cannot be coated with it. Coating with PVA becomes possible if one region of the hydrophobic functionality is polar. We describe a low-bleed HILIC stationary phase, PVA-coated benzylthioethyl-silica. The benzyl groups shield the silica from water erosion, while the thiol group provides sufficient local polarity for PVA to wet the material. The new stationary phase demonstrated good chromatographic performance and typical HILIC retention behavior. The measured silica concentration in the effluent was ∼80-fold lower than that from a bare silica column. The new stationary phase exhibited a lower background level, lower background noise, and lower background drift under gradient conditions than benchmark commercial columns.

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Preparation of a low bleeding polar stationary phase for hydrophilic interaction liquid chromatography

Cited by 21 publications

References 15 publications

A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

Recent Advances in Chromatography for Pharmaceutical Analysis

Low-Bleed Silica-Based Stationary Phase for Hydrophilic Interaction Liquid Chromatography

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