Preparation of a biocompatible albumin‐coated ion exchange resin for bilirubin removal from the blood of jaundiced newborns

Abstract

Removal of bilirubin by hemoperfusion with ion exchange resin particles is suggested to replace exchange transfusion of blood of jaundiced infants in some cases of unconjugated hyperbilirubinemia. The hemoperfusion system developed here consists of a packed bed of a macroreticular resin which is made biocompatible by a coating of a monomolecular layer of albumin. The choice of the appropriate ionic form of the resin and the proper albumin coating and crosslinking procedure assures a high bilirubin adsorption capacity and excellent blood compatibility of the resin. The albumin coated resin removes in vitro 80-90% of the bilirubin initially present in the plasma. The results encourage in vivo clinical studies.

Preparation of chitosan/amino multiwalled carbon nanotubes nanocomposite beads for bilirubin adsorption in hemoperfusion

“…In order to study the influence of adsorbents on blood components, fresh anticoagulant blood from healthy rabbits (2 mL for each sample) were mixed with different adsorbents (200 mg for each sample), respectively . All the adsorbents were fully swelled overnight in physiological saline.…”

Functionalized Carbon Nanotube-Embedded Poly(vinyl alcohol) Microspheres for Efficient Removal of Tumor Necrosis Factor-α

“…After incubating at 37 °C for 60 min, blood routine was determined using an Automatic Blood Cell Analyzer BC2600 (Shenzhen, China). 46 Fresh blood from a healthy rabbit was anticoagulated by sodium citrate, and the supernatant plasma was collected by centrifugation (3000 rpm, 10 min). Then, 2 mL of anticoagulated plasma was incubated with 0.1 g of the microspheres at 37 °C with gentle shaking for 1 h. The adsorbents were removed by centrifugation (4600g, 5 min), and the coagulation assays of the obtained supernatant were carried out using a Precil C2000-4 Coagulation System.…”

Preparation of PVA/amino multi-walled carbon nanotubes nanocomposite microspheres for endotoxin adsorption

“…In order to study the influence of microspheres on blood components, fresh anticoagulant blood from healthy rabbits (2 ml for each sample) were mixed with different microspheres (200 mg for each sample), respectively [28]. All the adsorbents were fully swelled overnight in physiological saline.…”