Preparation, Mechanical and Biological Properties of Inkjet Printed Alginate/Gelatin Hydrogel

Jiao, Tian; Lian, Qin; Zhao, Tingze; Wang, Huichao; Li, Dichen

doi:10.1007/s42235-021-0036-9

Cited by 12 publications

(7 citation statements)

References 43 publications

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“…Another common approach to reinforce soft hydrogel structures is to combine them within three-dimensional (3D) porous scaffolds. Such 3D support structures can be simultaneously printed with the hydrogel material, using techniques based on extrusion ,− or inkjet. , A novel technique to create 3D fiber reinforced scaffolds is melt electrowriting (MEW) . MEW allows for the generation of micrometer scale fibers that mechanically reinforce hydrogels while only encompassing a low volume percentage of the eventual construct .…”

Section: Introductionmentioning

confidence: 99%

Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Viola,

Ainsworth,

Mihajlovic

et al. 2024

Biomacromolecules

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Hydrogels are ideal materials to encapsulate cells, making them suitable for applications in tissue engineering and regenerative medicine. However, they generally do not possess adequate mechanical strength to functionally replace human tissues, and therefore they often need to be combined with reinforcing structures. While the interaction at the interface between the hydrogel and reinforcing structure is imperative for mechanical function and subsequent biological performance, this interaction is often overlooked. Melt electrowriting enables the production of reinforcing microscale fibers that can be effectively integrated with hydrogels. Yet, studies on the interaction between these micrometer scale fibers and hydrogels are limited. Here, we explored the influence of covalent interfacial interactions between reinforcing structures and silk fibroin methacryloyl hydrogels (silkMA) on the mechanical properties of the construct and cartilage-specific matrix production in vitro. For this, melt electrowritten fibers of a thermoplastic polymer blend (poly(hydroxymethylglycolide-co-ε-caprolactone):poly(ε-caprolactone) (pHMGCL:PCL)) were compared to those of the respective methacrylated polymer blend pMHMGCL:PCL as reinforcing structures. Photopolymerization of the methacrylate groups, present in both silkMA and pMHMGCL, was used to generate hybrid materials. Covalent bonding between the pMHMGCL:PCL blend and silkMA hydrogels resulted in an elastic response to the application of torque. In addition, an improved resistance was observed to compression (∼3-fold) and traction (∼40−55%) by the scaffolds with covalent links at the interface compared to those without these interactions. Biologically, both types of scaffolds (pHMGCL:PCL and pMHMGCL:PCL) showed similar levels of viability and metabolic activity, also compared to frequently used PCL. Moreover, articular cartilage progenitor cells embedded within the reinforced silkMA hydrogel were able to form a cartilage-like matrix after 28 days of in vitro culture. This study shows that hybrid cartilage constructs can be engineered with tunable mechanical properties by grafting silkMA hydrogels covalently to pMHMGCL:PCL blend microfibers at the interface.

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Section: Introductionmentioning

confidence: 99%

Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Viola,

Ainsworth,

Mihajlovic

et al. 2024

Biomacromolecules

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“…Xu et al selected inkjet-based bioprinting to successfully fabricate vascular-like constructs with both vertical and horizontal configurations . Jiao et al inkjet-printed the bioink with a dual-step cross-linking mechanism into soft tissues with enhanced mechanical properties demonstrating the feasibility of inkjet-based bioprinting for the fabrication of native tissues …”

Section: Introductionmentioning

confidence: 99%

“…24 Jiao et al inkjet-printed the bioink with a dual-step cross-linking mechanism into soft tissues with enhanced mechanical properties demonstrating the feasibility of inkjet-based bioprinting for the fabrication of native tissues. 25 As a key element in 3D bioprinting, bioink typically contains biological materials whose function is to mimic natural extracellular matrix (ECM) promoting cellular activities such as cell proliferation and differentiation 26,27 and living cells. Suitable biomaterials should possess good printability, biocompatibility, and biodegradability.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Cell Aggregation on the Printing Performance in Inkjet-Based Bioprinting of Cell-Laden Bioink

Liu

Shahriar

et al. 2022

Langmuir

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During 3D bioprinting, when the gravitational force exceeds the buoyant force, cell sedimentation will be induced, resulting in local cell concentration change and cell aggregation which affect the printing performance. This paper aims at studying and quantifying cell aggregation and its effects on the droplet formation process during inkjet-based bioprinting and cell distribution after inkjet-based bioprinting. The major conclusions of this study are as follows: (1) Cell aggregation is a significant challenge during inkjet-based bioprinting by observing the percentage of individual cells after different printing times. In addition, as polymer concentration increases, the cell aggregation is suppressed. (2) As printing time and cell aggregation increase, the ligament length and droplet velocity generally decrease first and then increase due to the initial increase and subsequent decrease of the viscous effect. (3) As the printing time increases, both the maximum number of cells within one microsphere and the mean cell number have a significant increase, especially for low polymer concentrations such as 0.5% (w/v). In addition, the increased rate is the highest using the lowest polymer concentration of 0.5% (w/v) because of its highest cell sedimentation velocity.

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“…Therefore, an edible hydrogel composed of sodium alginate and gelatin would benefit human health owing to its high protein, low digestible carbohydrate, zero fat, and low calorie contents. However, such hydrogels are not yet considered as functional foods due to their complex preparation process, the harmful additives required for gel synthesis, and poor mechanical properties 25,26 . In our previous work, we developed a pure gelatin and sodium alginate hydrogel through a simple dry‐swell process that only required the addition of acetic acid.…”

Section: Introductionmentioning

confidence: 99%

“…However, such hydrogels are not yet considered as functional foods due to their complex preparation process, the harmful additives required for gel synthesis, and poor mechanical properties. 25,26 In our previous work, we developed a pure gelatin and sodium alginate hydrogel through a simple dry-swell process that only required the addition of acetic acid. The resulting gel exhibits excellent mechanical properties (mechanical strength of up to 0.42 MPa) and a water content of $79%.…”

mentioning

confidence: 99%

Edible hydrogel from gelatin and alginate as functional low‐calorie noodle

Wang

Zhang

Wang

et al. 2022

J of Applied Polymer Sci

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There is an urgent need to develop edible hydrogels that can be served as a main dish with low‐calorie content and high satiety value, yet are easy to digest. However, the development of such hydrogels remains challenging. In this study, low‐calorie edible hydrogels were prepared from gelatin and sodium alginate (gel/SA hydrogels) through a facile acid soaking‐drying method. These gel/SA hydrogels exhibited excellent mechanical properties with texture characteristics similar to that of Liangpi. The sensitivity of gelatin and acidified sodium alginate to pH resulted in a high swelling ratio of the hydrogels, contributing to weight retention in a simulated stomach environment and rapid degradation in a simulated intestinal environment. These properties indicate that these gel/SA hydrogels have a good prospect of being used as low‐calorie food with high satiety value and are easy to digest. Konjac glucomannan can also be incorporated into the gel/SA hydrogels to further improve their biological functionality.

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Preparation, Mechanical and Biological Properties of Inkjet Printed Alginate/Gelatin Hydrogel

Cited by 12 publications

References 43 publications

Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Investigation of Cell Aggregation on the Printing Performance in Inkjet-Based Bioprinting of Cell-Laden Bioink

Edible hydrogel from gelatin and alginate as functional low‐calorie noodle

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