Preparation, development, and scale-up of standardized pentacyclic triterpenoid-rich extract from Centella asiatica (L.) Urb. and study of its wound healing activity

Rachpirom, Mingkwan; Pichayakorn, Wiwat; Puttarak, Panupong

doi:10.1016/j.heliyon.2023.e17807

Cited by 10 publications

(11 citation statements)

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“…was extracted with ultrasonic assistance and purified with macroporous resin to obtain triterpenes with a content of 68.11%. 27 In our study, the purity of triterpenes extracted by ultrasonic-assisted ethanol extraction was 29%, which was too low for the activity test. Macroporous resin was used to purify the triterpenes extracted from pomegranate peel.…”

Section: Discussionmentioning

confidence: 62%

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Zhang,

Guo

et al. 2024

Food Funct.

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Section: Discussionmentioning

confidence: 62%

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Zhang,

Guo

et al. 2024

Food Funct.

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“…The optimization of the microwave-assisted extraction method was achieved using Minitab Version 19.2 (Minitab Inc., State College, PA, USA) through the implementation of the Box-Behnken design (BBD) within the framework of response surface methodology (RSM) [22]. This study employed 4 independent variables (solvent ratio (X 1 ), extraction power (X 2 ), extraction time (X 3 ), and EtOH ratio (X 4 )), with each variable having three levels (high (1), medium (0), and low (-1)) chosen based on a suitable extraction method (2.3).…”

Section: Optimization Of a Suitable Extraction Methodsmentioning

confidence: 99%

“…The EtOH ratio was also examined for the suitable extraction method, which ranged from 25-75% EtOH in water (Table 1). We applied variable conditions based on the results from our previous study, which demonstrated that microwave energy could enhance the efficiency of the extraction method, resulting in reduced time and energy consumption compared to conventional methods [22]. The outcomes of the designed experimental study utilizing the BBD to evaluate the impact of 4 variables (X 1 , X 2 , X 3 , and X 4 ) are summarized in Table 1, which encompasses 27 experiments (F1 to F27).…”

Section: Optimization Of a Suitable Extraction Methodsmentioning

confidence: 99%

“…The anti-inflammatory activity was evaluated by the inhibition of LPS-induced nitric oxide (NO) production from murine macrophages (RAW264.7) [22]. RAW264.7 cells were cultured in a CO 2 incubator at 37 • C using RPMI medium supplemented with 10% fetal bovine serum (FBS), 0.1% sodium bicarbonate, 2 mM glutamine, and a penicillinstreptomycin solution (100 µg/mL).…”

Section: Bioactivity Determination Of Standardized Mangiferin-rich Ma...mentioning

confidence: 99%

“…The MTT assay was used to evaluate HDF cell proliferation at 37 • C in a humidified incubator containing 5% CO 2 according to the report of Rachpirom and coworkers [22]. HDF cells (2 × 10 3 cells/well) were seeded in DMEM containing 10% FBS for 24 h. Cells were then treated with or without the sample (using 1% DMSO as solvent) in DMEM containing 2% FBS for 48 h. Then, the supernatant was replaced with 100 µL of fresh media containing 10% MTT solution (5 mg/mL in PBS) for 2 h. Afterward, the medium was replaced with 150 µL of DMSO to dissolve the precipitate of formazan crystals of living cells.…”

Section: In Vitro Wound Healing Assay Cell Proliferationmentioning

confidence: 99%

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Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Sumpavapol,

Waehayee,

Suklim

et al. 2023

Sustainability

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The light mango or “Ma-Muang Bao” (Mangifere indica L. var.) is a native mango species originating from Malaysia and southern Thailand. The whole Ma-Muang Bao fruit, except peels, is popular as both a raw and ripe fruit for consumption, as well as being used in various processed food products. This study aims to transform the peel of a specific mango variety, which is a byproduct of the agro-industrial sweet, pickled mangoes industry, into a valuable bioactive ingredient for healthcare products. This is achieved through the establishment of a standardized mangiferin-rich mango peel extract (SMPE). Employing the Box–Behnken design (BBD) within the framework of response surface methodology (RSM), an optimal microwave-assisted extraction procedure was developed. A total of 27 experiments, each with four independent variables, including solvent ratio, extraction power, extraction time, and ethanol (EtOH) ratio, were conducted to optimize the extraction method in terms of mangiferin content and extraction yield. The optimized extraction conditions encompassed a solvent ratio of 120 mL EtOH/100 g sample, an extraction power of 450 W, an extraction time of approximately 4.3 min, and an EtOH ratio of 69.44% (EtOH in water). Small-scale extractions were carried out using the following specified parameters: solvent ratio of 120 mL, extraction power of 450 W, extraction time of 4 min, and EtOH ratio of 70% EtOH. These extractions yielded an extract with a mangiferin content of 27.24 ± 2.05 mg/g and an extraction yield of 3.71 ± 0.17% w/w. Notably, these outcomes were better from the mangiferin content of 19.62 mg/g and a yield of fresh peel of 5.61% estimated through BBD analysis. Furthermore, a pilot-scale extraction was performed using 7 kg of fresh mango peel and 70% EtOH (8.4 L) for 4 min, resulting in an extract with a mangiferin content of 51.85 ± 0.35 mg/g and a fresh peel yield of 4.35% w/w. This method emerges as the most suitable for mango peel extraction and forms the basis of the SMPE. The results from biological activities highlight the potential use of SMPE as the active ingredient for cosmeceutical or healthcare products for wound-healing and skin-brightening agents. Additionally, the knowledge from this study presents an alternative approach to various plant sources and sustainable extraction methods for the herbal extract industry.

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Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

Noppradit,

Klinnawee,

Leeratiwong

et al. 2024

Phytochemical Analysis

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IntroductionCentella is an important genus in the Apiaceae family. It includes Centella asiatica, which has significant edible and medicinal values. However, this species is easily confused due to its similar morphological traits to Hydrocotyle umbellata, hindering its utilization in the consumer and pharmacological industries.ObjectiveThe study aims to differentiate these two closely related plant species using reliable methods of confirming the authenticity of natural herbal medicines.MethodsOur work mainly focuses on the basic morphological characteristics, chemical markers, genetic fingerprints, and their biological responses.ResultsThe plants can be clearly differentiated using their leaf shapes, stipules, petioles, inflorescences, and fruit structures. Although the phytochemical compositions of the C. asiatica extract were similar to that of H. umbellata which included flavonoids, tannins, and saponins important to the plant's ability to reduce inflammation and promote healing of wounds, the H. umbellata extract showed significantly higher toxicity than that of C. asiatica. High‐performance liquid chromatography analysis was used to identify chemical fingerprints. The result revealed that C. asiatica had major triterpene glycoside constituents including asiaticoside, asiatic acid, madecassoside, and madecassic acid, which have a wide range of medicinal values. In contrast, triterpenoid saponins were not identified in H. umbellata. Furthermore, using SCoT1–6 primers was possible to effectively and sufficiently created a dendrogram which successfully identified the closeness of the plants and confirmed the differences between the two plant species.ConclusionTherefore, differentiation can be achieved through the combination of morphometrics, molecular bioactivity, and chemical analysis.

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Preparation, development, and scale-up of standardized pentacyclic triterpenoid-rich extract from Centella asiatica (L.) Urb. and study of its wound healing activity

Cited by 10 publications

References 48 publications

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

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