Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA Microspheres

Abstract

Cathelicidin-BF-30 (BF-30), a water-soluble peptide isolated from the snake venom of Bungarus fasciatus containing 30 amino acid residues, was incorporated in poly(D,L-lactide-co-glycolide) (PLGA) 75∶25 microspheres (MS) prepared by a water in oil in water W/O/W emulsification solvent extraction method. The aim of this work was to investigate the stability of BF-30 after encapsulation. D-trehalose was used as an excipient to stabilize the peptide. The MS obtained were mostly under 2 µm in size and the encapsulation efficiency was 88.50±1.29%. The secondary structure of the peptide released in vitro was determined to be nearly the same as the native peptide using Circular Dichroism (CD). The ability of BF-30 to inhibit the growth of Escherichia coli was also maintained. The cellular relative growth and hemolysis rates were 92.16±3.55% and 3.52±0.45% respectively.

Decorin-loaded poly lactic-co-glycolic acid nanoparticles modified by anti-alpha fetoprotein antibody: preparation, proliferation inhibition and induced apoptosis effects on HepG2 cells in vitro

“…There are two major signalling pathways in the process of apoptosis: one is the death receptor ligand signalling pathway, which is the ligand binding with the receptor of cell membrane to active various apoptotic precursors, thereby inducing apoptosis, and the other is the mitochondrial signalling pathway, which is disrupting mitochondrial structure and function, promoting apoptosis molecules to make the mitochondria release cytochrome into the cytoplasm and influencing a range of biological properties of cell so as to die . Regulating the expression of apoptosis‐related proteins can induce apoptosis.…”

Design and biological evaluation of novel BF-30 analogs for the treatment of malignant melanoma

“…Wang et al previously demonstrated that native BF-30 may selectively inhibit the proliferation of melanoma cells via in vitro permeabilization of cytoplasmic membrane and DNA-binding, and exerted the outstanding anti-cancer effects in B16F10-bearing mice 4 . However, native BF-30 was restricted in a short lifespan which mainly caused by the rapid glomerular filtration and protease digestion 5 .…”

Systemic Responses of Multidrug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Following Exposure to the Antimicrobial Peptide Cathelicidin-BF Imply Multiple Intracellular Targets

“…Emerging resistance to colistin and tigecycline, two of the few choices for last-resort treatment of P. aeruginosa and A. baumannii , may make this situation worse (Cai et al, 2012 ; Deng et al, 2014 ; Potron et al, 2015 ; Lee et al, 2016 ). Some antimicrobial peptides (AMPs) have shown excellent effects on these drug-resistant pathogens in vitro but are not able to be administered systemically due to their shortcomings, such as hemolytic activity or poor stability in vivo (Ramamoorthy et al, 2006 ; Zetterberg et al, 2011 ; Vila-Farres et al, 2012 ; Li et al, 2014 ; Liu et al, 2015 ). Though efforts have been made, mainly based on structural design, to overcome these disadvantages, no AMP is clinically available to date (Fjell et al, 2011 ; Andres, 2012 ).…”