Preparation, characterization, in‐vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with anticancer drug

Abstract

Biodegradable and biocompatible polymers that are engineered to nanostructures play a key role in providing solution for sustained chemotherapy. This study is focused on preparation, drug encapsulation efficiency, in-vitro drug release, in-vitro cellular uptake and cell viability of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) formulation containing vinblastine as the anticancer drug. Drug-loaded PGD NPs were prepared by a modified oil/water emulsion method and characterized by laser light scattering, atomic force microscopy (AFM), and zeta potential. The drug encapsulation efficiency was determined spectrophotometrically and in-vitro drug release was estimated using dialysis bag. Breast cancer cell line (MCF-7) was used to image and measure the cellular uptake of fluorescent PGD NPs. Cancer cell viability was assessed by treating MCF-7 cells with vinblastine-loaded PGD NPs by crystal violet staining method. Result showed that the vinblastine-loaded PGD NPs were superior in properties such as drug encapsulation efficiency, the cellular uptake and the cancer cell mortality.

Nanocarriers for optimizing the balance between interfollicular permeation and follicular uptake of topically applied clobetasol to minimize adverse effects

“…A way to minimize the risk of developing this adverse event would be to limit exposure of the skin to high concentrations by releasing the drug in a controlled manner. From previous works of our group and others it is known that polymeric nanoparticles can act as controlled drug release systems [14][15][16][17][18]. Hence, a sustained release of drug and avoidance of a burst effect would protect the epidermal epithelial regenerative cells from high drug concentrations, resulting in a reduced risk of developing local skin atrophy and enhanced regeneration.…”

Poly ε-caprolactone nanoparticles loaded with Uncaria tomentosa extract: preparation, characterization, and optimization using the Box–Behnken design

“…Most publications that have explored the development of NPs loaded with natural compounds have reported the use of isolated substances, with EEs obtained in these formulations remaining only near 50%, 19,[22][23][24] particularly when the natural component loaded was not extremely lipophilic. In contrast, the use of BBD as the chosen DOE method was effective for obtaining optimal formulations for the development of NPs that possess good EEs.…”

“…Moreover, it has been shown that while PCL NPs are biodegradable, extended drug release is maintained. 20,22 Such features, along with novel drug-targeting approaches, will make the NPs studied here a promising therapeutic for cancer treatment, especially given that UT extract has already demonstrated proven anti-tumorigenic activities.…”

“…Many of these formulation studies have reported entrapment efficiency (EE) values of around 50%. 19,[22][23][24] Special attention is being given to the process of loading natural anti-tumorigenic substances into NP systems; "nanochemoprevention" is thus becoming an increasingly explored concept worldwide. 25 For NP development, it is important that drug delivery systems have predictable release behavior and size distribution and reproducible drug loading.…”

Nanostructured delivery system for zinc phthalocyanine: preparation, characterization, and phototoxicity study against human lung adenocarcinoma A549 cells

“…9,[25][26][27] This polymer has been used in the development of release systems such as microparticles and NPs. [25][26][27] PCL NPs have been used as delivery systems of isradipine, 28 primidone, 29 paclitaxel, 30 griseofulvin, 31 tamoxifen, 32 espironolactone, 33 vinblastine, 34 and docetaxel. 35 The aim of this study was to load ZnPc in PCL NPs to improve the photobiological activity of the photosensitizer.…”