Preparation, Characterization Evaluation of Tanshinone IIA Lipid Microspere and Comparative Study of Pharmacokinetics with Sodium Tanshinone IIA Sulfonate Injection

Abstract

In this study, Tanshinone IIA lipid microspere (Tan-IIA-LM) was successfully prepared by an ultrasonic and high-pressure homogenization method. The particle size, zeta potential, drug entrapment efficiency and morphological properties of Tan-IIA-LM were characterized in detail. A pharmacokinetic comparative study of Tan-IIA-LM with Sodium Tanshinone IIA Silate (STS) injection was developed. A sensitive and specific HPLC method was established to determine the mass concentration of Tan-IIA and STS in rats plasma samples which were compared between Tan-IIA-LM and STS injection after single dose intravenous administration of 18.35μmol/kg and the results were fitted and analyzed by dedicated software. After intravenous administration, the AUC0-and Cmaxvalues of Tan-IIA were 2.14, 2.22 fold higher than STS. The clearance, apparent volume of distribution and mean residence time (MRT) of Tan-IIA were lower (P<0.01) than STS; other pharmacokinetic parameters had no significant deviation (P>0.05). The pharmacokinetic of Tan-IIA, STS have significant differences at the same molar doses and the AUC and Cmaxvalues of Tan-IIA were higher compared with STS.

CXCR4-Receptor-Targeted Liposomes for the Treatment of Peritoneal Fibrosis

“…The flow was set to 1 mL/min. The separation was achieved using a diamond ODS C18 column (150 mm × 4.6 mm × 5 μm). − The drug encapsulation efficiency (EE %) of STS IIA and AMD3100 was analyzed according to equation eq where W e was the analyzed concentration of entrapped STS IIA and AMD3100 in STS-AMD-lips and W t was the total analyzed concentration of STS IIA and AMD3100.…”

“…At predetermined time intervals (0.17, 0.5, 1, 2, 4, 8, 12, and 24 h), blood sample was taken in heparin tubes and centrifuged for 10 min at 5000 rpm. The plasma supernatant was isolated carefully and analyzed with HPLC. , PK-Solver was used to calculate the pharmacokinetic parameters.…”

“…The plasma supernatant was isolated carefully and analyzed with HPLC. 43,45 PK-Solver was used to calculate the pharmacokinetic parameters.…”

Progress in the Application of Chinese and Western Medicine in the Treatment of Chronic Osteomyelitis

“…And the relevant animal experiments showed that the use of total flavonoids in bone defect animal models treated with Masquelet technique can effectively promote the blood vessel formation and osteogenesis rate in the bone defect area. Wang Jiang et al [19] applied sodium tanshinone IIA sulfonate to the rabbit mandibular distraction osteogenesis model, and the results showed that the quality and rate of new bone formation in the distraction gap of the experimental group of animals were better than that of the control group during the same period.…”

Recent insights into the biological activities and drug delivery systems of tanshinones

“…However, nanoemulsions have proved to have higher bioaccessibility, stability, and optical clarity than conventional emulsions; 91,92 nanoemulsions are similar to microemulsions that are monodispersed spherical droplets with thermodynamic stability as well as excellent solubility properties. 93,94 In recent years, a series of studies have been reported regarding the preparation processes, quality control, and evaluation profiles of tanshinone microemulsions.…”