Preparation, Characterization, and Release Behavior of Doxorubicin hydrochloride from Dual Cross-Linked Chitosan/Alginate Hydrogel Beads

Wu, Ting; Yu, Shaobin; Lin, Dan; Wu, Zhimin; Xu, Jun; Zhang, Jinglin; Ding, Zefen; Miao, Ying; Liu, Tao; Chen, Tao; Cai, Xiang

doi:10.1021/acsabm.9b01119

Cited by 35 publications

(28 citation statements)

References 41 publications

(90 reference statements)

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“…This study represents the first attempt in the field of food packaging. As a consequence, a direct comparison with other studies from scientific literature dealing with drug delivery through beads is not very appropriate because in most cases, beads formulation consists of alginates blended with other polymeric materials to enhance the entrapment efficacy [44,45], or cross-linked with chitosan [46]. Similarly, examples of chitosan films incorporating generally recognized as safe (GRAS) antimicrobials as sodium benzoate are found in the scientific literature; however, these studies are not focused on the kinetic release of SB but rather on exclusively assessing the effect of active chitosan films on target microorganisms in model systems or real food [47][48][49].…”

Section: Resultsmentioning

confidence: 99%

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Conte

Lecce

Iannetti

et al. 2020

Foods

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The influence of film structure on the release kinetics of sodium benzoate (SB) from polymeric films is addressed in this study. In particular, four film structures were investigated, two monolayer and two multilayer systems. In particular, in one case, the active substance was uniformly distributed into a chitosan-based matrix, and in the other one, it was previously incorporated into alginate beads before dispersion in the chitosan film, thus realizing two types of monolayer films; on the other hand, the same chitosan film with SB encapsulated in alginate beads was used as the inner layer of a multilayer system constituted by two side films of alginate. The two alginate-based layers were made with two different thicknesses, thus producing a total of two multilayer systems. The release of SB from the above-mentioned films in water was studied by means of a UV/VIS spectrophotometer at 227 nm. A first-order kinetics-type equation was used to quantitatively describe the release data. Results suggest that the film structure strongly affected the release kinetics. In fact, monolayer films showed single-stage release kinetics, whereas the two investigated multilayer systems showed two-stage release kinetics. Further, the presence of alginate beads strongly affected the SB release, thus suggesting the potential of encapsulation to control the release mechanism of active compounds.

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Section: Resultsmentioning

confidence: 99%

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Conte

Lecce

Iannetti

et al. 2020

Foods

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“…84,85 These aggregations come from high concentrations of the used chitosan and alginate and/or high molecular weight of these natural polymers. 86,87 In order to bypass these problems, it would be better to use the independent nanocarriers to the polymeric structure. It means using nanocarriers that are not dependent on the polymeric structures to perform their application.…”

Section: Methodsmentioning

confidence: 99%

Turning Toxic Nanomaterials into a Safe and Bioactive Nanocarrier for Co-delivery of DOX/pCRISPR

Rabiee

Bagherzadeh

Ghadiri

et al. 2021

ACS Appl. Bio Mater.

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Hybrid bioactive inorganic−organic carbon-based nanocomposites of reduced graphene oxide (rGO) nanosheets enlarged with multi-walled carbon nanotubes (MWCNTs) were decorated to provide a suitable space for in situ growth of CoNi 2 S 4 and green-synthesized ZnO nanoparticles. The ensuing nanocarrier supplied π−π interactions between the DOX drug and a stabilizing agent derived from leaf extracts on the surface of ZnO nanoparticles and hydrogen bonds; gene delivery of (p)CRISPR was also facilitated by chitosan and alginate renewable macromolecules. Also, these polymers can inhibit the potential interactions between the inorganic parts and cellular membranes to reduce the potential cytotoxicity. Nanocomposite/nanocarrier analyses and sustained DOX delivery (cytotoxicity analyses on HEK-293, PC12, HepG2, and HeLa cell lines after 24, 48, and 72 h) were indicative of an acceptable cell viability of up to 91.4 and 78.8% after 48 at low and high concentrations of 0.1 and 10 μg/mL, respectively. The MTT results indicate that by addition of DOX to the nanostructures, the relative cell viability increased after 72 h of treatment; since the inorganic compartments, specifically CoNi 2 S 4 , are toxic, this is a promising route to increase the bioavailability of the nanocarrier before reaching the targeted cells. Nanosystems were tagged with (p)CRISPR for co-transfer of the drug/genes, where confocal laser scanning microscopy (CLSM) pictures of the 4′,6-diamidino-2phenylindole (DAPI) were indicative of appropriate localization of DOX into the nanostructure with effective cell and drug delivery at varied pH. Also, the intrinsic toxicity of CoNi 2 S 4 does not affect the morphology of the cells, which is a breakthrough. Furthermore, the CLSM images of the HEK-293 and HeLa cell displayed effective transport of (p)CRISPR into the cells with an enhanced green fluorescent protein (EGFP) of up to 8.3% for the HEK-293 cell line and 21.4% for the HeLa cell line, a record. Additionally, the specific morphology of the nanosystems before and after the drug/gene transport events, via images by TEM and FESEM, revealed an intact morphology for these biopolymers and their complete degradation after long-time usage.

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“…In particular, encapsulation of anthracyclines in vesicles, specifically doxorubicin (DOX), has the potential to decrease the toxicity of the agents. DOX is an antimicotic and cytotoxic agent that intercalates in DNA and disrupts topoisomerase‐II mediated DNA repair [27,28] …”

Section: Introductionmentioning

confidence: 99%

Highly Stable Nanostructured Magnetic Vesicles as Doxorubicin Carriers for Field‐assisted Therapies

et al. 2022

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In this work, magnetic vesicles were produced using magnetite (Fe 3 O 4 ) nanoparticles and 1-methylimidazolium bis-(2-ethylhexyl) phosphate (imim-DEHP) as surfactant. Some of the vesicles were subsequently coated with chitosan to improve their stability and biocompatibility. A physicochemical characterization of the prepared systems was carried out using several techniques, which allowed verifying that the magnetic nanoparticles were successfully encapsulated. Finally, the vesicles were loaded with an antitumor drug (doxorubicin) by swelling in order to perform release studies. The Korsmeyer-Peppas model was used to fit the doxorubicin (Dox) release profiles in the different vesicle systems, which displayed delayed release in every case. After 24 h, the Dox release percentages ranged between 43-53%. The prepared vesicles show not only excellent stability but also a good response to an external magnetic field, which makes them good candidates to be used in field-assisted therapies, thus trying to improve therapeutic efficacy and avoiding possible adverse effects.

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Preparation, Characterization, and Release Behavior of Doxorubicin hydrochloride from Dual Cross-Linked Chitosan/Alginate Hydrogel Beads

Cited by 35 publications

References 41 publications

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Turning Toxic Nanomaterials into a Safe and Bioactive Nanocarrier for Co-delivery of DOX/pCRISPR

Highly Stable Nanostructured Magnetic Vesicles as Doxorubicin Carriers for Field‐assisted Therapies

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