Preparation, Characterization and ex vivo Skin Permeability Evaluation of Type I Collagen-Loaded Liposomes

Abstract

Purpose In the present study, we prepared collagen liposomes with the addition of polyol, which is expected to not only increase the solubility of collagen but also improve skin penetration. Methods Collagen liposomes were prepared by the film dispersion method, and their characteristics, integrity and biosafety were evaluated by Fourier transform infrared spectroscopy (FTIR), UV-VIS spectroscopy, polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscope (TEM). The transdermal absorption of collagen and collagen liposomes were tested by an ex vivo horizontal Valia-Chien diffusion cell system. Results We first demonstrated that collagen extracted from bovine Achilles tendon was type I collagen. The results of DLS measurement and TEM observation showed that the collagen liposomes were spherical in shape with average diameter (75.34±0.93 nm) and maintained high stability at low temperature (4°C) for at least 42 days without toxicity. The encapsulation rate of collagen liposomes was 57.80 ± 0.51%, and SDS-PAGE analysis showed that collagen was intact in liposomes. Finally, permeability studies indicated that the collagen-loaded liposomes more easily penetrated the skin compared to collagen itself. Conclusion This study proposed a new method to improve the bioavailability and permeability of bovine type I collagen, which improves the applicability of collagen in biomedicine, cosmeceuticals and pharmaceutical industries.

Microcurrent Cloth-Assisted Transdermal Penetration and Follicular Ducts Escape of Curcumin-Loaded Micelles for Enhanced Wound Healing

“…29,30 Bioactive nanoparticles and nanocomposites have recently been demonstrated as potential applications to accelerate wound healing [31][32][33] using nanotechnology, including polymer-drug conjugates, polymer micelles, nanoparticles, composite particles, self-assembled lipid nanoparticles and liposomes for hydrophobic compounds delivery. [34][35][36] It is reported that Cur can be encapsulated in surfactant micelles to enhance its water solubility/dispersity. 37 Additionally, Cur nanoparticles with different sizes affect the therapeutic efficacy of curcumin.…”

Key Transdermal Patch Using Cannabidiol-Loaded Nanocarriers with Better Pharmacokinetics in vivo

“…[33][34][35][36][37][38] Several nanocarrier formulations have been developed to enhance transdermal drug delivery, including liposomes, ethosomes, transfersomes, polymeric micelles, selfnanoemulsifying drug delivery system (SNEDDS), solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLCs), and polymeric nanoparticles. [39][40][41][42][43][44] Despite the growing popularity of TDDS, there have been limited developments in formulations specifically for CBD. Nanocarriers formulation provides a promising platform for enhancing the delivery of CBD.…”

Ex vivo Evaluation of a Liposome-Mediated Antioxidant Delivery System on Markers of Skin Photoaging and Skin Penetration

“… 27 For example, the topical application of solid lipid nanoparticles and nanostructured lipid carrier systems have been utilized with active compounds such as vitamin E (tocopherol), 28 retinoic acid, 29 and more. 30 , 31 Additionally, liposomes have been utilized to load anti-aging compounds such as type I collagen, 32 ferulic acid, 33 apigenin and doxycycline, 34 polyvinyl alcohol and gelatin, 35 and ligustrazine hydrochloride, 36 all of which have shown improved photoprotective effects with the utilization of the liposomal technology. Complexing lipids with polyphenols, termed lipophenols, have also been shown to be effective.…”