Preparation, characterization and cytotoxic applications of curcumin‐(±) α‐lipoic acid coloaded phosphorylated chitosan nanoparticles in MDA MB 231 breast cancer cell line

Abstract

In the present work, curcumin in combination with (±) α‐Lipoic acid were used as the active agents for the preparation of phosphorylated chitosan nanoparticle. Ionic gelation method was used for the successful fabrication of the nanoparticles. Effective incorporation of the drugs in the polymeric system was revealed by Fourier‐transform infrared spectroscopy (FT‐IR) and X‐ray diffractometry (XRD). Scanning electron microscope, transmission electron microscope and dynamic light scattering method were used to analyze the morphology and dimensional characteristics of the nanoparticles. The swelling degree and drug release mechanism were studied at pH 5.0 and 7.4 for different time periods by UV‐visible spectrophotometer. Cytotoxicity study was performed by MTT assay analysis on normal human lymphocyte and MDA MB 231 breast cancer cell line. Trypan blue dye exclusion assay was performed to confirm the anticancer effect of the nanoparticles. The properties of the prepared nanoparticles recommended their possible application as anticancer agents.

Study on Chitosan Nanoparticles in Liver Cancer

“…An effective reduction in proteins that promote colon cancer proliferation. Our results suggest that the siRNA-(FITC-CHC) -CMC delivery system has great potential for for RNAi therapy in diseased cells [15] .…”

Carvacrol-Fabricated Chitosan Nanoparticle Synergistic Potential with Topoisomerase Inhibitors on Breast and Cervical Cancer Cells

“…The percentage yield of freeze-dried nanoparticles was determined as described by the formula percentage yield = normalweight of the nanoparticles normalweight of .25em ( drug + polymer ) × 100 % …”

Phosphorylation of chitin and chitosan: A review on its significance and various synthesis routes

“…[56][57][58] Incorporation of phosphate functionalities in the chitin and chitosan backbone is of great interest nowadays due to their application in numerous fields such as tissue engineering, [59][60][61] flame retardants, [62][63][64] wastewater treatment, [65][66][67][68] fuel cells, 69,70 and drug delivery. 71,72 Few reviews have been reported in the literature 73,74 that summarize the different methods of phosphorylation of chitosan; however, they lack a comparative study so that the reader can choose the appropriate method. This particular review is novel in terms of the fact that, firstly, in this paper, the importance of introducing phosphorus-containing functional groups into the structure of polysaccharides (especially chitin and chitosan) is highlighted.…”

“…56–58 Incorporation of phosphate functionalities in the chitin and chitosan backbone is of great interest nowadays due to their application in numerous fields such as tissue engineering, 59–61 flame retardants, 62–64 wastewater treatment, 65–68 fuel cells, 69,70 and drug delivery. 71,72…”