Preparation, characterization and biological properties of a novel bone block composed of platelet rich fibrin and a deproteinized bovine bone mineral

Feng, Mengge; Wang, Yulan; Wei, Yan; Zhang, Xiaoxin; Gong, Zijian; Fujioka‐Kobayashi, Masako; Sculean, Anton; Miron, Richard J.; Froum, Scott H; Zhang, Yufeng

doi:10.1016/j.fmre.2021.08.003

Cited by 14 publications

(24 citation statements)

References 37 publications

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“…H-PRF gels from each temperature group were also put in six-well cell culture plates immediately after preparation, then the six-well plates were tilted at 45 degrees every 15 s to observe whether the H-PRF gel would dissolve/flow. If the H-PRF gel wouldn’t dissolve, the mixture was deemed to be completely solidified and a corresponding solidification time was recorded as previously described [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Photos and weighs of the H-PRF gel were recorded every day. The photos revealed the ability for the H-PRF gel to either stay intact or dissolve over time [ 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the samples were coated with gold automatically. The surface of each group was then captured by SEM (VEGA 3 LMU, TESCAN, Brno, Czech Republic) and investigated for morphological differences [ 11 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

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Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel

et al. 2022

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Purpose Platelet-rich fibrin (PRF) has been proposed as promising biomaterials with the advantages of host accumulation of platelets and leukocytes with entrapment of growth factors and fibrin scaffold. However, limitations including fast resorption rate (~ 2 weeks) restricts its clinical application. Recent studies have demonstrated heating treatment can prolong PRF degradation. Current published articles used the method of 75 °C for 10 min to obtain longer degradation, while few studies investigated the most suitable temperature for heating horizontal PRF. Our present study was to discover and confirm the optimum temperature for heat treatment before obtaining H-PRF gels by investigating their structure, mechanical properties, and bioactivity of the H-PRF gels after heating treatment. Methods In the present study, 2-mL upper layer of horizontal PRF was collected and heated at 45 °C, 60 °C, 75 °C, and 90 °C to heat 2-mL upper layer of horizontal PRF for 10 min before mixing with the 2-mL lower layer horizontal PRF. The weight, solidification time and the degradation properties were subsequently recorded. Scanning electron microscopy (SEM) and rheologic tests were carried out to investigate the microstructure and rheologic properties of each H-PRF gel. The biological activity of each H-PRF gel was also evaluated using live/dead staining. Results H-PRF gel prepared at 75 °C for 10 min had the fast solidification period (over a tenfold increase than control) as well as the best resistance to degradation. The number of living cells in H-PRF gel is greater than 90%. SEM showed that H-PRF gel becomes denser as the heating temperature increases, and rheologic tests also revealed that the heat treatment improved the mechanical properties of H-PRF gels when compared to non-heated control group. Future clinical studies are needed to further support the clinical application of H-PRF gels in tissue regeneration procedures. Conclusions Our results demonstrated that the H-PRF gel obtained at 75 °C for 10 min could produce a uniform, moldable gel with a short time for solidification time, great rheologic behavior and, high percent of live cells in PRF gel. A promising use of the commonly utilized PRF gel was achieved facilitating tissue regeneration and preventing degradation.

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Section: Methodsmentioning

confidence: 99%

“…Photos and weighs of the H-PRF gel were recorded every day. The photos revealed the ability for the H-PRF gel to either stay intact or dissolve over time [ 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

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Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel

et al. 2022

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“…To prepare the H-PRF extracts, each treated H-PRF membrane was incubated with 5 ml DMEM (Dulbecco's modi ed Eagle's medium) supplemented with 10% FCS (fetal calf serum) and 1% penicillin G (Thermo Fisher Scienti c Inc.) in a 37℃ incubator for 3 days as previous described. [11] Then, the medium was collected, ltered with 0.22 µm membrane lter (Millex-GP, PR05538, Merck Millipore Ltd.) and stored at 4℃ for future cell experiments. In order to avoid the insigni cant differences between the groups due to excessive cell proliferation in the later stage, human osteoblasts (hFOBs) suspension was counted by Invitrogen Countess 3 (Thermo Fisher Scienti c) and then were diluted to 1,0000 cells per milliliter and then seeded 100 µL/well in 96-well plates as previous study recommended.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Effect of thermal manipulation on the biological and mechanical characteristics of horizontal platelet rich fibrin membranes

Yu²,

Wang³

et al. 2023

Preprint

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Purpose Regardless of application scenarios, proper mechanical characteristics and degradation property is the prerequisite for horizontal platelet rich fibrin (H-PRF) to manifest its ability. Among the methods used to modify PRF, thermal manipulation is promising as it is easy to handle without adding extra additives. Yet there is no consensus on optimal temperature treatment. The present study aimed to investigate the effect of heating on the biological and mechanical characteristics and explore the most suitable heating temperature for H-PRF thermal treatment.Methods We employed a series of temperature gradients, room temperature, 50℃, 75℃, 90℃, 105℃. The microstructure and the mechanical properties were recorded by Scanning Electron Microscope (SEM) and tensile strength tests respectively. The degradation rate of H-PRF membranes was examined by digestion assay with plasmin and trypsin. The viability of cells within H-PRF membranes and the proliferation of osteoblasts cultured with extracts from different H-PRF groups was evaluated using CCK-8 assays.Results Compared with the nonheated group, over-heated manipulation beyond 90℃ can significantly prolong the degradation properties up to 3 to 4 weeks and enhance the mass stress of H-PRF membranes. A high temperature treatment of 105℃ accompanied with the cell activity beneath H-PRF reduced more than half and thus the biological effect to human osteoblasts (hFOBs) also reduced dramatically.Conclusions High thermal manipulation can prolong the degradation properties and enhance the mechanical properties of PRF membranes while accompanied by the loss of biological effect.

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“…[5][6][7] Also, it is already possible to observe studies suggesting the possibility of using such an osteoconductive biomaterial. 8 The recent finds show the Alb-PRF as an autologous promisor biomaterial. Our group are grateful to contribute to the science behind that.…”

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confidence: 99%

Alb-PRF: the history behind the science

Mourão

Calasans-Maia²,

Alves³

2022

IJCBR

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Preparation, characterization and biological properties of a novel bone block composed of platelet rich fibrin and a deproteinized bovine bone mineral

Cited by 14 publications

References 37 publications

Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel

Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel

Effect of thermal manipulation on the biological and mechanical characteristics of horizontal platelet rich fibrin membranes

Alb-PRF: the history behind the science

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