Preparation, Characterization and Application of a Molecularly Imprinted Polymer for Selective Recognition of Sulpiride

Abstract

A novel molecular imprinting polymer (MIP) was prepared by bulk polymerization using sulpiride as the template molecule, itaconic acid (ITA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the crosslinker. The formation of the MIP was determined as the molar ratio of sulpiride-ITA-EGDMA of 1:4:15 by single-factor experiments. The MIP showed good adsorption property with imprinting factor α of 5.36 and maximum adsorption capacity of 61.13 μmol/g, and was characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR) and surface area analysis. With the structural analogs (amisulpride, tiapride, lidocaine and cisapride) and small molecules containing a mono-functional group (p-toluenesulfonamide, formamide and 1-methylpyrrolidine) as substrates, static adsorption, kinetic adsorption, and rebinding experiments were also performed to investigate the selective adsorption ability, kinetic characteristic, and recognition mechanism of the MIP. A serial study suggested that the highly selective recognition ability of the MIP mainly depended on binding sites provided by N-functional groups of amide and amine. Moreover, the MIP as solid-phase extractant was successfully applied to extraction of sulpiride from the mixed solution (consisted of p-toluenesulfonamide, sulfamethoxazole, sulfanilamide, p-nitroaniline, acetanilide and trimethoprim) and serum sample, and extraction recoveries ranged from 81.57% to 86.63%. The tentative tests of drug release in stimulated intestinal fluid (pH 6.8) demonstrated that the tablet with the MIP–sulpiride could obviously inhibit sulpiride release rate. Thus, ITA-based MIP is an efficient and promising alternative to solid-phase adsorbent for extraction of sulpiride and removal of interferences in biosample analysis, and could be used as a potential carrier for controlled drug release.

Development of an active packaging with molecularly imprinted polymers for beef preservation

“…Molecular imprinting technology (MIT) may be an important development direction of the active packaging because it can process a variety of formats with tunable sorption/release behaviors . As we know, MIT is good at forming selective sites in a polymer matrix with the memory of a template and enables the use of stimuli‐responsive molecularly imprinted polymers (MIPs) materials as smart chemicals and drug‐delivery systems .…”

“…6 Molecular imprinting technology (MIT) may be an important development direction of the active packaging because it can process a variety of formats with tunable sorption/release behaviors. [7][8][9][10] As we know, MIT is good at forming selective sites in a polymer matrix with the memory of a template and enables the use of stimuli-responsive molecularly imprinted polymers (MIPs) materials as smart chemicals 11,12 and drug-delivery systems. 13,14 The template release kinetics of MIPs indicates their potential for controlled release drug dosage, 15,16 and the increase in release rate at higher loadings is attributed to partial drug adsorption to nonspecific non-specific binding sites with weak attachment, typical release patterns of MIPs morphology were determined, and MIPs particles in solvents can be decomposed in 3 fractions.…”

Adsorption of malachite green onto Styrene-Methacrylate based molecularly imprinted polymer

“…Where [S] represents the quantity of dye AIBN. The stretching band of C=O at 1724 cm affirms the structural characteristic of EGDMA (Zhang et al 2017). All the spectra have a very -1 strong and sharp peak at 1637 cm , which may be attributed to the existence of a large number of olefinic carbon-carbons (C=C) bonds of MG, styrene and EGDMA (Wang et al 2009;Khasanah et al 2010).…”

Cost-Effective Potentiometric Platforms Modified with Multi-Walled Carbon Nanotubes (MWCNTs) and Based on Imprinted Receptors for Fluvoxamine Assessment

“…It can be seen that Kd and Qmax values were 55.3µmol/L and 485.9 µmol/g for high affinity and 110.9 µmol/L, 237.2 µmol/g for low-affinity binding sites of MIP, respectively. Two linear regression equations indicate that two different binding sites for FLV were formed in MIP, which are known as specific and non-specific binding sites from imprinting cavities [50]. In contrast, the Kd and Qmax values were 781.4 µmol/L and 129.4 µmol/g for NIP, respectively.…”