Preparation, Characterization, and Antioxidant Activity Evaluation of Liposomes Containing Water-Soluble Hydroxytyrosol from Olive

Yuan, Jiaojiao; Qin, Frank G.F.; Tu, Junling; Li, Bing

doi:10.3390/molecules22060870

Cited by 30 publications

(21 citation statements)

References 35 publications

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“…Pinsuwan et al (79) demonstrated the enhanced antioxidant activity of liposomes encapsulating extract of Hibiscus sabdariffa using an in vitro skin model. A similar in vitro activity enhancement using liposome-based encapsulation of natural products has been reported by other researchers using different methods (78,80). Feng et al evaluated the in vivo effect of liposomal encapsulation of chlorogenic acid following oral administration in mice.…”

Section: Antioxidant Assaysupporting

confidence: 65%

Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols

et al. 2020

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This work aims to improve the functionality of Rosmarinus officinalis L. (rosemary) polyphenols by encapsulation in an optimized proliposome formulation. A 2 3 Box-Wilson central composite design (CCD) was employed to determine lone and interaction effects of composition variables on moisture content (X p); water activity (A w); concentration and retention of rosemary polyphenols-rosmarinic acid (ROA), carnosol (CAR), and carnosic acid (CNA); and recovery of spray-dried proliposomes (SDP). Processing conditions which generate proliposomes with optimum physicochemical properties were determined by multi-response analysis (desirability approach). Antioxidant and antifungal activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH •) sequestering and minimum inhibitory concentration (MIC)/minimum fungicidal concentration (MFC) assays, respectively. SDP exhibited high polyphenol retention, ranging from 62.0 to 100.0% w/w, showing dependence on composition variables and polyphenol lipophilicity. SDP recovery ranged from 20.1 to 45.8%, with X p and A w of 1.7 ± 0.14-2.5 ± 0.23% w/w and 0.30 ± 0.004-0.47 ± 0.003, respectively, evidencing product with good chemical and microbiological stability. Optimum liposomal composition was determined, namely, lipid concentration (4.26% w/w), lyophilized extract (LE) concentration (4.48% w/w), and drying aid:(lipid+extract) ratio (7.55% w/w) on wet basis. Relative errors between experimental and predicted values for SDP properties showed concurrence for all responses except CAR retention, being 22% lower. SDP showed high antioxidant activity with IC 50 of 9.2 ± 0.2 μg/ mL, superior to results obtained for LE (10.8 μg/mL) and butylated hydroxytoluene (BHT), a synthetic antioxidant (12.5 μg/mL). MIC and MFC against Candida albicans (ATCC1023) were 312.5 μg/mL and 1250 μg/mL, respectively, a moderate antimicrobial activity for phytochemical-based products. SDP is shown as a veritable tool to encapsulate hydrophilic and lipophilic rosemary polyphenols generating a product with optimal physicochemical and biological properties.

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Section: Antioxidant Assaysupporting

confidence: 65%

Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols

et al. 2020

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“…The maximum LC value was obtained at the ratio of 8:1. Many reports have demonstrated that the stability and EE of liposomes could be enhanced by adding an appropriate amount of Tween 80 in the formulation (Lu et al., ; Yuan, Qin, Tu, & Li, ). This might be attributed to the steric stabilization provided by Tween 80.…”

Section: Resultsmentioning

confidence: 99%

Co‐Encapsulation of EGCG and Quercetin in Liposomes for Optimum Antioxidant Activity

Chen

Zou

et al. 2018

Journal of Food Science

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Although different delivery systems have been developed to overcome the limits of Epigallocatechin‐3‐gallate (EGCG) and quercetin in food application, none have referred to their simultaneous encapsulation. In this study, these two polyphenols were successfully co‐loaded into liposomes. Under the optimal conditions (lecithin‐total polyphenols ratio 25:1, lecithin‐cholesterol ratio 6:1, lecithin‐Tween 80 ratio 8:1 and ultrasonic time 2 min), the mean size, polydispersity index (PDI) and zeta potential of liposomes were 111.10 ± 0.52 nm, 0.259 ± 0.006 and –19.83 ± 0.45 mV, with an encapsulation efficiency of 64.05 ± 1.56% and 61.73 ± 2.55% for EGCG and quercetin, respectively. After 30‐day storage, an increase of 4.05% was observed in the mean size with no significant change (P ≥ 0.05) in the PDI and zeta potential. Moreover, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay revealed a synergistic antioxidant effect of the two compounds in liposomal system. These results demonstrated that co‐encapsulation of EGCG and quercetin enhances their effectiveness. Practical Application EGCG and quercetin are natural polyphenols abound in the human diet with diverse biological activities. These two polyphenols were successfully co‐encapsulated into a homogeneous and stable liposomal system. Interestingly, a synergistic antioxidant effect of the two polyphenols was observed due to co‐encapsulation. This indicated that the simultaneous delivery of EGCG and quercetin was an attractive approach to improve their functionality for expanding their application in food, cosmetic and pharmaceutical industries.

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“…Liposomes are spherical phospholipid bilayers that mimic cellular structures and can be used as model membrane systems. They are widely used as xenobiotic delivery systems since they improve the availability of the active compound, target and slow its release, and show protective features [ 44 ]. This is why liposomes have potential applications in a wide range of fields, including pharmaceutical sciences, food technology, or agriculture.…”

Section: Resultsmentioning

confidence: 99%

Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

et al. 2018

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Waste remaining after the production of olive oil (olive pomace) is known to contain significant amounts of phenolic compounds that exert different types of biological activities, primarily acting as antioxidants. In this work, a sustainable approach that combines ultrasound-assisted extraction with food-grade solvents and encapsulation with different types of cyclodextrins was used to prepare olive pomace-based polyphenol rich extracts that were tested as antioxidants in various chemical, food, and biological model systems. Encapsulation with cyclodextrins had a significant positive impact on the chemical composition of obtained extracts and it positively affected their antioxidant activity. Observed effects can be explained by an increased content of polyphenols in the formulations, specific physical properties of encapsulated compounds improving their antioxidant activity in complex food/physiological environment, and enhanced interaction with natural substrates. Depending on the applied model, the tested samples showed significant antioxidant protection in the concentration range 0.1–3%. Among the investigated cyclodextrins, hydroxypropyl-β-cyclodextrin and randomly methylated-β-cyclodextrin encapsulated extracts showed particularly good antioxidant activity and were especially potent in oil-in-water emulsion systems (1242 mg/g and 1422 mg/g of Trolox equivalents, respectively), showing significantly higher antioxidant activity than Trolox (reference antioxidant). In other models, they provided antioxidant protection comparable to commonly used synthetic antioxidants at concentration levels of 2–3%.

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Preparation, Characterization, and Antioxidant Activity Evaluation of Liposomes Containing Water-Soluble Hydroxytyrosol from Olive

Cited by 30 publications

References 35 publications

Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols

Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols

Co‐Encapsulation of EGCG and Quercetin in Liposomes for Optimum Antioxidant Activity

Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

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