Preparation, characterization, and antibacterial properties of biocompatible material for wound healing

Solak, Ebru Kondolot; Kaya, Sami; Asman, Gülsen

doi:10.1080/10601325.2021.1929315

Cited by 12 publications

(3 citation statements)

References 22 publications

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“…Silver sulfadiazine and phenytoin ointment were usually used as standard medicine groups [ 47 – 49 ]. Some other studies used mupirocin and povidone-iodine ointment [ 50 , 51 ]. In this study, a 21-day trial was conducted to evaluate wound healing in the in vivo and silver sulfadiazine condition selected as standard medicine.…”

Section: Discussionmentioning

confidence: 99%

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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Background and Objectives. A wound is one of the high-prevalence disorders that affect people’s lives physically, mentally, and financially. This study examined the Astragalus microcephalus Willd. wound healing process in in vivo and in vitro conditions by focusing on the phytoestrogen activity of this extract. Methods. The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats’ control groups included silver sulfadiazine, Vaseline, and the nontreated groups. Results. Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. Conclusion. This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue.

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Section: Discussionmentioning

confidence: 99%

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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“…Staphylococcus aureus is an important opportunistic pathogen normally found on human skin and nasal mucosa. It is known to be responsible for nosocomial infections [40]. It can also cause pneumonia, endocarditis, meningitis and infection in wounds [41].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles and Comprehensive Characterization for Biomedical Applications

Doğan,

Kaya,

Solak

2024

Preprint

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This study details the preparation and extraction process of Origanum onites plant extract collected from Denizli, Turkey. The leaves were meticulously cleaned, dried, and ground before undergoing extraction in a water-ethanol solution using a Soxhlet Apparatus at 100 ºC for 6 h. The resulting extract was evaporated and stored for future use at + 4°C. Afterwards, the extract was utilized in the green synthesis of silver nanoparticles by adding it to a solution of silver nitrate. The impact of temperature on the synthesis of silver nanoparticles was explored across various temperature values (30 ºC, 60 ºC, and 90 ºC). Furthermore, the influence of reaction time on silver nanoparticle formation was investigated at the determined optimum temperature, with examination periods set at 60, 120, and 180 minutes. The influence of temperature on the antioxidant activity and cytotoxicity of the synthesized nanoparticles has been explored across three distinct temperature values. Characterization of the Silver Nanoparticles (AgNPs) included UV–Vis Spectrophotometry for surface plasmon resonance, Scanning Electron Microscopy (SEM) for morphological analysis, Energy Dispersive X-ray analysis (EDX) for elemental composition, Particle size distribution and Fourier-transform infrared spectroscopy (FT-IR) spectroscopy for interpreting chemical bonds and functional groups.

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“…Sodium alginate is also widely used in tissue engineering, wound care, and pharmaceutical chemistry due to its physicochemical properties as suspension and emulsification, thickening, thermo-stability, colloids, and film formation. [5][6][7] Another biodegradable natural polymer collagen is the most clinically effective material for wound healing and skin regeneration. Collagen is the main component of the dermal layers of the skin and accelerates wound healing.…”

Section: Introductionmentioning

confidence: 99%

Quercetin‐loaded sodium alginate/collagen/h‐boron nitride potential wound dressings prepared using the Box‐Behnken experimental design

Çakıcı,

Kaya,

Doğan

et al. 2023

Biotechnology Journal

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Background/aimsNatural and synthetic biocompatible polymers have received significant attention in the pharmaceutical industry due to their rapid and effective healing properties in the wound healing process. The aim of this study was to optimize the extraction of onions, the preparation of sodium alginate/collagen/hydrogen boron nitride (NaAlg/Col/h‐BN) membranes using the Box‐Behnken experimental design, and determine the optimal conditions for quercetin release. The study also aimed to investigate the antimicrobial and antioxidant activities of the prepared membranes and their therapeutic properties.Methods and resultsThe prepared membranes were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), Differential Scanning Calorimetry (DSC), and X‐ray diffraction (XRD). Antimicrobial activities were tested against Gram‐negative (Gr‐) Escherichia coli ATCC 25922, Klebsiella pneumonia, Enterobacter aerogenes, Gram‐positive (Gr+) Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 pathogens. In vitro release studies were conducted to examine the therapeutic properties of the prepared membranes. The optimum conditions for the extraction of onions and the preparation of NaAlg/Col/h‐BN membranes were found to be EtOH = 75 mL, t = 2 h, T = 45°C, and NaAlg = 1.0 g, Col = 2.0 g, and h‐BN = 6% wt, respectively. The prepared membranes exhibited serious antimicrobial properties against S. aureus and C. albicans. The membranes also promoted the controlled release of quercetin for 24 hours in vitro, indicating their potential as a new approach in wound treatment.ConclusionThe study concludes that quercetin‐filled NaAlg/Col/h‐BN membranes have promising therapeutic properties for wound healing. The membranes exhibited significant antimicrobial and antioxidant properties, and their controlled release of quercetin suggests their potential for use in wound healing applications.This article is protected by copyright. All rights reserved

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Preparation, characterization, and antibacterial properties of biocompatible material for wound healing

Cited by 12 publications

References 22 publications

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

Green Synthesis of Silver Nanoparticles and Comprehensive Characterization for Biomedical Applications

Quercetin‐loaded sodium alginate/collagen/h‐boron nitride potential wound dressings prepared using the Box‐Behnken experimental design

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