Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

Yang, Yiyuan; Wu, Mingzheng; Wegener, Amy J.; Vázquez‐Guardado, Abraham; Efimov, Andrew I; Lie, Ferrona; Wang, Taoyi; Ma, Yuhang; Banks, Anthony; Xie, Zhaoqian; Good, Cameron H.; Kozorovitskiy, Yevgenia; Rogers, John A.

doi:10.1038/s41596-021-00672-5

Cited by 19 publications

(11 citation statements)

References 23 publications

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“…Devices were implanted using a single standard surgical procedure 64 . Animals were anesthetized using isoflurane (3% for induction, 1.5-2% for anesthesia maintenance) and their head fur was shaved.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

Vázquez‐Guardado

et al. 2022

Nat Commun

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In vivo optogenetics and photopharmacology are two techniques for controlling neuronal activity that have immense potential in neuroscience research. Their applications in tether-free groups of animals have been limited in part due to tools availability. Here, we present a wireless, battery-free, programable multilateral optofluidic platform with user-selected modalities for optogenetics, pharmacology and photopharmacology. This system features mechanically compliant microfluidic and electronic interconnects, capabilities for dynamic control over the rates of drug delivery and real-time programmability, simultaneously for up to 256 separate devices in a single cage environment. Our behavioral experiments demonstrate control of motor behaviors in grouped mice through in vivo optogenetics with co-located gene delivery and controlled photolysis of caged glutamate. These optofluidic systems may expand the scope of wireless techniques to study neural processing in animal models.

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Section: Surgical Proceduresmentioning

confidence: 99%

Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

Vázquez‐Guardado

et al. 2022

Nat Commun

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“…In contrast to the smooth surface before, SMSO particles after the BID process exhibited much rougher surfaces, featuring nanostructures such as particles and troughs with sizes ≤100 nm, similar to those found during natural demineralization. During surface roughening, every single micrometer-sized SMSO particle shed ~10 6 nanoparticles into the solution (see Supplementary Text).…”

Section: Mechanistic Study Of the Bid Technique For Producing Colloid...mentioning

confidence: 99%

“…Besides conventionally used external light sources, internally delivered light sources represent an arising opportunity to mitigate the challenges associated with the poor tissue penetration of photons. On the one hand, nanofabricated light sources in a flexible and stretchable platform provide a minimally invasive strategy for delivering an internal light source in vivo ( 6 , 7 ). On the other hand, microparticles and nanoparticles with persistent luminescence, also known as the afterglow, represent another potential approach for internal light delivery owing to their ability to store photon energy in their chemical structures.…”

Section: Introductionmentioning

confidence: 99%

A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source

Yang

Cui

et al. 2022

Sci. Adv.

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Many in vivo biological techniques, such as fluorescence imaging, photodynamic therapy, and optogenetics, require light delivery into biological tissues. The limited tissue penetration of visible light discourages the use of external light sources and calls for the development of light sources that can be delivered in vivo. A promising material for internal light delivery is persistent phosphors; however, there is a scarcity of materials with strong persistent luminescence of visible light in a stable colloid to facilitate systemic delivery in vivo. Here, we used a bioinspired demineralization (BID) strategy to synthesize stable colloidal solutions of solid-state phosphors in the range of 470 to 650 nm and diameters down to 20 nm. The exceptional brightness of BID-produced colloids enables their utility as multicolor luminescent tags in vivo with favorable biocompatibility. Because of their stable dispersion in water, BID-produced nanophosphors can be delivered systemically, acting as an intravascular colloidal light source to internally excite genetically encoded fluorescent reporters within the mouse brain.

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“…In place with the dramatic technological changes and upgrades in the age of intelligence, the integration of the information networks of the human life and the physical world has become a pressing need to explore electronic technologies to acquire real-time data. [1,2] Wearable electronic devices, which evolved from the conventional heavy and cumbersome ones to become intelligent and mobile appliances with prolific applications ranging from health monitoring to biomedical treatment, entertainment, environmental protection, homeland security, human-machine interaction, computing, and robotics, have sparked a great deal of scholarly and industrial attention. [3,4] Wearable power sources with the convenient-to-wear and effective-to-discharge features are virtually necessary and critical to achieve the proper, smooth, and onbody operation of the wearable electronic devices without the need for a fixed infrastructure, leading to improved mission execution of the electronics under the circumstances of daily routine using.…”

Section: Introductionmentioning

confidence: 99%

Flexible Biofuel Cell‐In‐A‐Tube (iezTube): An Entirely Self‐Contained Biofuel Cell for Wearable Green Bio‐energy Harvesting

Wang

Sun

Pei

et al. 2022

Adv Funct Materials

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Wearable biofuel cells (BFCs) technologies have the potential to address the challenge of on‐body energy supply of wearable electronics. However, previous wearable BFCs primarily focus on “worn‐on‐skin” sweat ones, which can hardly directly generate steady and nonintermittent electricity under certain but almost ineluctable wearable circumstances (e.g., intermittent sweating, perspiration biofuel concentration fluctuation, and dynamic contact between wearable BFCs and epidermal sweat). Here, in an alternative “worn‐close to‐skin” manner, the first example of a flexible BFC‐in‐a‐tube (named iezTube) as an entirely self‐contained wearable BFC (ESW‐BFC) for wearable energy generation by integrating a single‐layer fluff pulp (FP)‐based microfluidic module for the efficient sampling/utilization of biofuel fluids and the steady contact between biofuel fluids and wearable BFC with a flexible nano‐engineered BFC‐based BFC module for real‐time bio‐energy generation, along with an air‐breathing module that is based on a breathable and waterproof non‐woven tapes covered centrifuge tube‐based for continuous oxidizer supply, is reported. Through the seamless system integration, effective series‐connection pattern, and diverse readily accessible biofuel fluids pre‐collection, the iezTube demonstrates an exceptional capability to steadily and nonintermittently power wearable electronics under some certain but nearly ineluctable wearable circumstances and holds considerable and inimitable prospects as an ESW‐BFC for wearable electronics.

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Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

Cited by 19 publications

References 23 publications

Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology

A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source

Flexible Biofuel Cell‐In‐A‐Tube (iezTube): An Entirely Self‐Contained Biofuel Cell for Wearable Green Bio‐energy Harvesting

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