Preparation and structure characterization of linear long‐chain dextrin obtained from pullulanase debranching of cassava starch

Abstract

A relatively simple and economical method to prepare linear long-chain dextrin (LLD) was created, with a resulting product that could act as a high-amylose starch for a variety of downstream applications. Native cassava starch was chosen as the starting material due to its inexpensive price and low-gelatinization temperature. A pretreatment with NaOH solution was applied to increase the susceptibility of cassava starch to pullulanase attack. The apparent amylose content of the final LLD product was extremely high (above 90%), as determined by the iodine binding colorimetric method. Using HPLC, the optimum amount of enzyme was determined to be 25 U pullulanase per gram of starch. The structure of the LLD was found to be a V6I crystalline type, and this was confirmed by multiple physical techniques, including X-ray powder diffraction, solid-state nuclear magnetic resonance, and Fourier transform infrared. Overall, this study could promote further investigations into the efficient preparation and characterization of amylose products.

Characteristics of starch‐based films produced using glycerol and 1‐butyl‐3‐methylimidazolium chloride as combined plasticizers

“…Cassava starch was purchased from Gldark Potato Modified Starch Co, Ltd (Gansu, P. R. China), and contained 13.4% moisture, 0.13% ash, and 0.08% crude protein. The apparent amylose content was 28.6% as measured previously . Glycerol (analytical reagent grade) was supplied by Lingfeng Chemical Reagent Co, Ltd (Shanghai, P. R. China).…”

Preparation and Characterization of Thermoplastic Potato Starch/Halloysite Nano-Biocomposites: Effect of Plasticizer Nature and Nanoclay Content

“…Native starch is a complex polysaccharide sourced from plants, composed of two types of α -glucans, linear amylose and highly-branched amylopectin. These biomacromolecules are organized into a highly complex semi-crystalline structure that results from the biosynthesis of the starch granules by the plant [5,6]. Neat starch exhibits high brittleness and poor mechanical properties.…”

A temperature‐mediated two‐step saccharification process enhances maltose yield from high‐concentration maltodextrin solutions

“…The residual substrate might be more susceptible to pullulanase. After pullulanase addition, debranching reaction catalyzed by pullulanase was therefore efficient due to its easy access to branch points 24 . Moreover, the linear chains released also provided a fresh substrate for β ‐amylase and increased maltose production within the remaining 40 h of the saccharification process (over 36%) (Fig.…”