Preparation and storage of “leukocyte‐depleted single donor” platelet concentrate: A step forward in effective haemotherapy

Elias, M. K.; Wolf, Jtm de; Blom, Nel R.; Rijskamp, L.; Halie, R. M.; Sibinga, C. Th. Smit

doi:10.1002/jca.2920060304

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…The impact of PC storage on post-transfusion platelet increment has been evaluated in several studies [5,6]. It has been assumed that SD-PC are superior to RDP regarding corrected count increments (CCI) after transfusion [7], but this subject remains questionable [8].…”

Section: Introductionmentioning

confidence: 99%

In-vivo evaluation of random donor platelet concentrates from pooled buffy coats

Klüter

Dörges

Maaß

et al. 1996

Annals of Hematology

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Random-donor platelet concentrates (PC) prepared from pooled buffy coats have recently been described as an alternative method for platelet preparation. We evaluated such PCs in the clinical setting compared with a standard PC from platelet apheresis. PCs were prepared either from pools of buffy coats (BC-PC) or from single donors (SD-PC) with the cell separator CS-3000 plus. PCs were stored for up to 5 days before transfusion. We compared fresh PC (day 1) with stored (day 2-3) and long-stored PC (day 4-5). For analysis, platelet increment in the recipient was determined immediately and 16-22 h (mean 20 h) after transfusion, corrected for total body area and transfused platelets (CCI). A total of 316 PCs were administered to 36 thrombocytopenic patients suffering from various hematological disorders. Patients with detectable HLA or platelet-specific antibodies or splenomegaly were excluded from the study. Mean platelet content of the PC was 262 x 10(9) for BC-PC and 251 x 10(9) for SD-PC. The 20-h CCI after transfusion of fresh PC was slightly higher with BC-PC than with SD-PC (14.5 versus 11.9; p = 0.19), but values did not differ significantly between the two types of PC on any day of storage. For BC-PC, 20-h CCI decreased with further storage by 30% (10.2; p = 0.02). For SD-PC a decrease by 9% was not significant. In conclusion, platelet concentrates prepared from pools of buffy coats showed excellent transfusion results when administered fresh, but storage decreased the CCI by 30%. No significant difference from PCs from plateletpheresis was observed on any day of storage. Both types of platelet concentrates were capable of sufficient platelet increment even when stored for up to 5 days.

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Section: Introductionmentioning

confidence: 99%

In-vivo evaluation of random donor platelet concentrates from pooled buffy coats

Klüter

Dörges

Maaß

et al. 1996

Annals of Hematology

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Evaluation of elutriated single donor platelets collected and stored in a closed system

Elias

Blom

Rijskamp

et al. 1992

J of Clinical Apheresis

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Single donor platelets (SDPC) were collected by the elutriation technique in a closed-system integrated with large storage containers. Seven runs of SDPC were stored in a 1.5 liter polyvinyl-chloride trimellitate (PVC-TOTM) storage container, making the ratio of platelet concentrate volume to container volume 1:4.5. An equal volume of pooled multiple donor platelet concentrates (MDPC) was stored in parallel under the same conditions. All haematological data were comparable for both products, except for the degree of leukocyte contamination (5-fold increase in the pool). Under these conditions, the functional, morphological, and metabolic characteristics of elutriated platelets throughout 7-day storage were superior to those of pooled platelets. Although the platelet count was not significantly different in both types of concentrates, the mean pH of pooled MDPC fell to 6.0 on day 5 of storage. Leukocytes were shown to contribute to this pH fall. The extent of cell damage, however, as evidenced by LDH leakage (42.7 LDH units/10(11) platelets/day by differential centrifugation, compared to 5.3 units by elutriation) could not be explained solely on the basis of the leukocyte effect. This indicated that the processing method itself influences the platelet quality. By increasing the surface/volume ratio of SDPC, the initial pH of 7.1 was well maintained throughout storage, platelet metabolic rate was slowed, and the function and ultrastructure improved significantly.

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Use of single donor platelets

Kirkley¹

1994

Blood Reviews

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Preparation and storage of “leukocyte‐depleted single donor” platelet concentrate: A step forward in effective haemotherapy

Cited by 4 publications

References 31 publications

In-vivo evaluation of random donor platelet concentrates from pooled buffy coats

In-vivo evaluation of random donor platelet concentrates from pooled buffy coats

Evaluation of elutriated single donor platelets collected and stored in a closed system

Use of single donor platelets

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