For the purpose of forming cells possessing more than three nuclei and of determining the factors inducing multinucleation, cells of Saccharomyces cerevisiae were treated with 0. 0.3. 0.5. and 1.0% [w/v] colchicine solution, with and without shaking. When the cells were treated with 1.0%[wlv] colchicine solution, the number of cells containiig two to eight nuclei was the largest. The multinucleate cells could grow on potato dextrose agar medium and their multinucleate nature did not disappear for at least thr…
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