Acta Biotechnol. volume 18, issue 4, P305-313 1998 DOI: 10.1002/abio.370180403 View full text
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H. Toyama, N. Toyama

Abstract: For the purpose of forming cells possessing more than three nuclei and of determining the factors inducing multinucleation, cells of Saccharomyces cerevisiae were treated with 0. 0.3. 0.5. and 1.0% [w/v] colchicine solution, with and without shaking. When the cells were treated with 1.0%[wlv] colchicine solution, the number of cells containiig two to eight nuclei was the largest. The multinucleate cells could grow on potato dextrose agar medium and their multinucleate nature did not disappear for at least thr…

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