Preparation and quality by design assisted (Qb-d) optimization of bioceramic loaded microspheres for periodontal delivery of doxycycline hyclate

Abstract

PLGA (Lactic- co-glycolic acid) coated chitosan microspheres loaded with hydroxyapatite and doxycycline hyclate complex were developed in the present study for periodontal delivery. A modified single emulsion method was adopted for the development of microspheres. Formulation was optimized on the basis of particle size, drug loading and encapsulation efficiency with the central composite design using 2 3 factorial design. Microspheres were optimized and electron microscopy revealed their spherical shape and porous nature. In-vitro study showed initial burst and then sustained release behavior of the formulation for 14 days. Further, in-vitro antibacterial study performed on E. coli (ATCC-25922) and S. aureus (ATCC-29213) revealed concentration dependent activity. Also, in-vitro cyto-toxicity assessment ensures biocompatibility of the formulation with the fibroblast’s cells. Overall, the quality by design assisted PLGA microspheres, demonstrated the desired attributes and were found suitable for periodontal drug delivery.

QbD-Assisted Development and Optimization of Doxycycline Hyclate- and Hydroxyapatite-Loaded Nanoparticles for Periodontal Delivery

“…The developed complex was characterized on the basis of surface morphology by SEM, so as to confirm the development of the complex. 38 Further, in order to calculate the amount of drug adhered to the HAP surface, a weighed amount of the HAP-Doxy dried composite was added to 10 mL of water. The suspension was then filtered with the help of Whatman filter paper, and the unadsorbed drug in solution was determined with a UV−visible spectrophotometer (UV-1601, Shimadzu, Japan) at 271.5 nm.…”

“…After complete drying, the adsorbed composite was stored in a cool and dry place. The developed complex was characterized on the basis of surface morphology by SEM, so as to confirm the development of the complex …”

“…All the samples (collected in triplicate) were filtered through a 0.45 μm syringe filter and analyzed through RP-HPLC. The results were reported as the mean ± SD of three replicates ( N = 3) . Further, the obtained data were analyzed, and the release kinetics for the drugs was determined.…”

In silico and in vitro assessment of an optimized QbD-guided myoinositol and metformin-loaded mucus-penetrating particle-based gel for the amelioration of PCOS

“…The high and low levels of the given factors were coded by +1 and −1, respectively, which were used to determine the relative impact of the independent variables compared to their coefficients. 42 3.3.1 Effect of the independent variables on the size of the particles. Particle size (PS) is considered to be a critical parameter in the CQAs in the fabrication of MPPs, given that it facilitates the penetration and release of the therapeutic drugs.…”

Bioceramics in Endodontics: Updates and Future Perspectives

“…In addition, the introduction of antibiotics or drugs into bionic bone scaffolds and the use of bioceramics and scaffolds to control their release can increase the antibacterial activity. Lactic-co-glycolic acid (PLGA)-coated chitosan microspheres loaded with HA and doxycycline hyclate complexes have been developed for periodontal delivery [ 298 ]. Endodontic sealers that incorporate novel, highly loaded antimicrobial drug-silica coassembled particles (DSPs) show great antimicrobial activity [ 299 ].…”