Preparation and purification of an immunoregulatory peptide from Stolephorus chinensis of the East Sea of China

Xu, Baogui; Ye, Lei; Tang, Yunping; Zheng, Jiawen; Tian, Xiaoxiao; Yang, Yunxia; Yang, Zuisu

doi:10.1016/j.procbio.2020.08.011

Cited by 18 publications

(14 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LMW peptides exhibit stronger immunomodulatory effects ( 23 , 25 ). Moreover, ingesting LMW peptides leads to enhanced intestinal absorption, resulting in higher bioavailability ( 26 , 27 ).…”

Section: Resultsmentioning

confidence: 99%

“…Yu et al ( 23 ) showed that LMW peptides (< 3 kDa) from Nibea japonica skin induced the highest proliferation of RAW264.7 cells compared with other fractions (3–5 kDa, 5–10 kDa, and > 10 kDa). Xu et al ( 25 ) showed that LMW peptides (< 1 kDa) from Stolephorus chinensis induced the highest proliferation of RAW264.7 cells, and subsequently isolated an immunoregulatory peptide, Tyr-Val-Met-Arg-Phe, from the LMW peptides. In this study, 88.27% of the total MRP had smaller peptides having MWs < 1 kDa, indicating their promising bioactivities.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

This study aimed to investigate the immunomodulatory activation of low-molecular-weight peptides from monkfish (Lophius litulon) roe (named MRP) on cyclophosphamide (CTX)-induced immunosuppressed mice. Our results indicated that MRP (100 mg/kg/d BW) could significantly increase the body weight and immune organ index, and improve the morphological changes in the spleen and thymus of mice. These effects subsequently enhance the serum levels of interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, and immunoglobulin (Ig) A, IgM, and IgG. Furthermore, MRP could also improve CTX-induced oxidative stress, and activate the NF-κB and MAPK pathways in the spleen tissues. The findings reported herein indicate that MRP has a good immunomodulatory activation toward immunosuppressed mice, hence can potentially be developed as an immune adjuvant or functional food.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The fractions below 1 kDa obtained from the membrane separation of both the Stolephorus chinensis hydrolysate [ 16 ] and the soybean hydrolysate [ 21 ] displayed the highest immune cell activity compared to the other fractions.…”

Section: Resultsmentioning

confidence: 99%

“…The macrophages were cultured in a medium (containing 90% DMEM medium, 10% FBS, and 1% double antibody) at 37 °C and 5% CO 2 . The MRPR was determined following to the MTT assay used by Xu et al [ 16 ]. The density of the log-phase cells was adjusted to 1 × 10 5 cells/mL, and 100 μL/well of cell suspension was seeded in 96-well plates and incubated overnight in an incubator (BB-150-CO2 incubator, Thermo Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Purification, Identification and Molecular Docking of Immunomodulatory Peptides from the Heads of Litopenaeus vannamei

Jiang

Yang

Zhou

et al. 2022

Foods

View full text Add to dashboard Cite

In order to realize the high-value utilization of Litopenaeus vannamei (L. vannamei) heads, immunomodulatory peptides were prepared from the enzymatic hydrolysate of L. vannamei heads, and the action mechanism of immunomodulatory peptides was determined by molecular docking. The results showed that six proteases were used to hydrolyze L. vannamei head proteins, with the animal protease hydrolysate exhibiting the highest macrophage relative proliferation rate (MRPR). The enzymatic products were then sequentially purified by ultrafiltration, Sephadex G-15 gel chromatography, identified by liquid chromatography-mass spectrometry (LC-MS/MS), and finally selected for six immunomodulatory peptides (PSPFPYFT, SAGFPEGF, GPQGPPGH, QGF, PGMR, and WQR). These peptides maintained good immune activity under heat treatment, pH treatment, and in vitro gastrointestinal digestion. Molecular docking analysis indicated that these peptides showed great binding to both toll-like receptor 2 and 4 (TLR2 and TLR4/MD-2), leading to immunomodulation. The discarded L. vannamei heads in this article are considered to be promising food-borne immunomodulators that contribute to enhancing the immune function of the body.

show abstract

“…Table 1. Summary of the techniques used for the preparation and purification of marine bioactive peptides commercially [72][73][74][75][76][77][78][79][80][81][82][83][84][85]. Choline-oxalic acid based on eutectic solvent * HPLC (High performance liquid chromatography), reverse-phase-high-performance liquid chromatography, RP-HPLC, mass spectrometry (MS), liquid chromatography followed by tandem mass spectrometric detection (LC-MS/MS), electrospray ionization (ESI), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD), ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), and liquid chromatography-tandem mass spectrometry (RRLC-MS).…”

Section: Techniques Used For the Commercial Preparation And Purificat...mentioning

confidence: 99%

Therapeutic Potential of Marine Bioactive Peptides against Human Immunodeficiency Virus: Recent Evidence, Challenges, and Future Trends

et al. 2022

View full text Add to dashboard Cite

Acquired immunodeficiency syndrome (AIDS) is a chronic and potentially fatal ailment caused by the human immunodeficiency virus (HIV) and remains a major health problem worldwide. In recent years, the research focus has shifted to a greater emphasis on complementing treatment regimens involving conventional antiretroviral (ARV) drug therapies with novel lead structures isolated from various marine organisms that have the potential to be utilized as therapeutics for the management of HIV-AIDS. The present review summarizes the recent developments regarding bioactive peptides sourced from various marine organisms. This includes a discussion encompassing the potential of these novel marine bioactive peptides with regard to antiretroviral activities against HIV, preparation, purification, and processing techniques, in addition to insight into the future trends with an emphasis on the potential of exploration and evaluation of novel peptides to be developed into effective antiretroviral drugs.

show abstract

Preparation and purification of an immunoregulatory peptide from Stolephorus chinensis of the East Sea of China

Cited by 18 publications

References 54 publications

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

Purification, Identification and Molecular Docking of Immunomodulatory Peptides from the Heads of Litopenaeus vannamei

Therapeutic Potential of Marine Bioactive Peptides against Human Immunodeficiency Virus: Recent Evidence, Challenges, and Future Trends

Contact Info

Product

Resources

About