“…It has been purified from some plant sources and characterized (Gibson & Ullah, 1988;Konietzny et al, 1995;Mahajan & Dua, 1997;Phillippy, 1998;Greiner et al, 1998Greiner et al, , 2001Greiner & Alminger 1999;Greiner, 2002;Andriotis & Ross, 2003;Belho et al, 2016;Belho & Ambasht, 2017). Immobilization of phytase has been pursued mostly from microbial sources like Aspergillus ficuum (Liu et al, 1999;Moraes et al, 2008), A. niger (Menezes-Blackburn et al, 2011;Trouillefou et al, 2015;Coutinho et al, 2020), A. heteromorphus (Lata et al, 2014), Escherichia coli (Menezes-Blackburn et al, 2011;Cho et al, 2011;El-Shora et al, 2019), Candida krusei (Quan et al 2003), and mushrooms (Onem & Nadaroglu, 2014;Onem et al, 2016). The literature on the enzyme immobilization, and the use of different matrices, has been extensively discussed (Mosbach, 1971;Mateo et al, 2007;Spahn & Minteer, 2008;Liu et al, 2018;Sastre et al, 2020).…”