Preparation and properties of monoclonal antibodies to the anabolic agent zeranol

Abstract

Monoclonal antibodies have been produced using two haptens, zeranol-7-hemisuccinate coupled to bovine serum albumin and zeranol-16-carboxypropyl ether coupled to human serum albumin. An assessment of cross-reactivity demonstrated that the monoclonal antibody raised against the 7-hemisuccinate derivative reacted with zeranol (100%), talernol (12%), zearalenone (17%), zearalanone (100%) and alpha- and beta-zearalenol (17% and less than 0.01%, respectively). In contrast the antibodies to the 16-carboxypropyl ether derivative reacted only with zeranol (100%) and also with a alpha-zearalenol (13-16%). All monoclonal antibodies were more specific than the polyclonal antibodies raised to the same haptens by conventional methods using sheep.

Determination of zeranol and its metabolites in bovine muscle and liver by a chemiluminescence enzyme immunoassay: compared to an ultraperformance liquid chromatography tandem mass spectroscopy method

“…MAbs against ZAL was prepared first by Carter et al . . In the current study, the myeloma/splenocyte fusion yielded two stable hybridomas producing MAbs against ZAL.…”

“…This fully exposes the difference between analogues. The raised antibodies toward 16‐position hapten are less cross‐reactive with analogues than that of 7‐position hapten .…”

Applications of Monoclonal Antibodies in Animal Health and Production

“…It has previously been mentioned that anabolic drugs may be employed as growth promoters in animals, and animal feed may be supplemented with antibiotics for prophylactic control of infectious disease. Carter et al (1984) prepared MAbs against the anabolic agent zeranol, and suggested appHcations of this antibody in immunoassays for monitoring residues of zeranol in animal products. Several authors have developed MAbs against antibiotics and suggested their use in immunoassays for detecting the presence of residual antibiotic in animal products, including Van de Water et al (1987), who used a MAb against chloramphenicol for development of a competitive ELISA for determination of chloramphenicol residues in swine muscle tissue.…”

A specificity-enhanced time-resolved fluoroimmunoassay for zeranol employing the dry reagent all-in-one-well principle

“…3 A number of immunochemical methods have been developed for the screening of zeranol. [4][5][6][7] However, all the current immunochemical tests exhibit cross-reactivity to a greater or lesser extent with zearalenone, a-zearalenol and b-zearalenol, the three principal toxins produced by Fusarium spp. fungi.…”