Preparation and Properties of a Hyphal Wall Fraction from Pithomyces chartarum

Abstract

SUMMARYA low-sporing isolate of Pithomyces chartarum was grown in submerged liquid culture in defined medium, and a hyphal wall fraction isolated by mechanical disruption. It contains about 20 yo protein, 40 yo bound hexoses, 10 yo bound glucosamine, 10 yo lipid and 5 yo ash.

Spore Surface Depsipeptides in Some Pithomyces Species

“…This was not the case, however, for, in other routine experiments, stirring the residue overnight with chloroform invariably removed considerable amounts of extractive. This material, which was readily and completely soluble in ether and may have been lipid derived from the cell wall (Russell, Sturgeon & Ward, 1964) or cytoplasm, was not examined further.…”