Preparation and processing of dried blood spots for microRNA sequencing
Alice Morgunova,
Pascal Ibrahim,
Gary Gang Chen
et al.
Abstract:Dried blood spots (DBS) are biological samples commonly collected from newborns and in geographic areas distanced from laboratory settings for the purposes of disease testing and identification. MicroRNAs – small non-coding RNAs that regulate gene activity at the post-transcriptional level – are emerging as critical markers and mediators of disease, including cancer, infectious diseases and mental disorders. This protocol describes optimized procedural steps for utilizing DBS as a reliable source of biological… Show more
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