Preparation and preservation of red cell concentrates in MAP solution by quadruple bag system.

In silico modeling and metabolome analysis of long-stored erythrocytes to improve blood storage methods

“…2B). Furthermore, intracellular adenine is reportedly depleted at three weeks of storage (Shiba et al, 1991), which was similarly found in our estimated model (data not shown).…”

“…In addition, the pH value gradually decreases due to lactate accumulation (Hogman et al, 1999). As the storage time-dependent pH variation of the model, we used the published time-course data of intracellular pH in commercially available cold-RC-MAP (Shiba et al, 1991). The initial pH value was set to be 6.95, and the decrease in intracellular pH during storage over 49 days was linear, with a first-order coefficient of 8.66 × 10 −8 s −1 .…”

“…Because the reaction activities in cold-stored RC-MAP could not be obtained from the literature, we divided the reaction activities into three groups of reaction properties and used GA to estimate these activities. These estimated activities served as adjustable parameters that were fitted to previously published (Shiba et al, 1991) time-course data of ATP and 2,3-BPG in commercially available RC-MAP at 4 • C for 49 days.…”

GVHD after transfusion of stored RBC concentrates in asolution of mannitol, adenine, phosphate, citrate, glucose,and NaCl following trauma

“…In addition, suspending the whole blood in MAP helps separate WBCs from RBCs. The number of lymphocytes decreases from 9.2 ± 3.9 × 10 8 per unit to 7.0 ± 2.0 × 10 7 per unit in the process of preparation 4 …”

“…In recent years, several additive solutions, such as saline‐adenine‐glucose‐mannitol (SAGM) and ADSOL, have been developed to store RBCs for longer periods 2,3 . A modified solution of SAGM, made up of mannitol, adenine, phosphate, citrate, glucose, and NaCI (MAP), is currently used in Japan because of its advantage over SAGM in the length of time it maintains flexibility of RBCs 4 …”