Preparation and preliminaryin vitroevaluation of a bFGF-releasing heparin-conjugated poly(ε-caprolactone) membrane for guided bone regeneration

Abstract

In an effort to improve guided bone regeneration (GBR), we successfully fabricated a novel basic fibroblast growth factor (bFGF)-releasing heparin-conjugated poly(ε-caprolactone) membrane (hep-PCL/bFGF). This material has a porous microstructure with smooth and rough pore walls before and after heparinization, respectively. Our FTIR analyses indicated that chemical bonds were formed between PCL and heparin with a new amide C=O band at 1660 cm(-1) and a band at 3400 cm(-1) that can be attributed to -OH stretching in cross-linked heparin. We showed that bFGF was released from hep-PCL/bFGF in a continuous pattern, which remained for 3 weeks. We evaluated MG63 cell proliferation and biocompatibility of GBR membrane by a CCK-8 assay and a live/dead assay. The CCK-8 results revealed that the hep-PCL/bFGF group had superiority compared to other groups. Furthermore, cell morphology of hep-PCL membrane exhibited larger projected areas than those of PCL surfaces based on scanning electron microscopy analysis and immunofluorescent staining of cell cytoskeleton and vinculin expression. Our alkaline phosphatase activity assay also confirmed better performance of the hep-PCL/bFGF group. These results suggested that this novel hep-PCL/bFGF membrane is suitable for osteoblast-like cells to attach, proliferate, and differentiate. Therefore, the hep-PCL/bFGF membrane has potential to be a biodegradable membrane for GBR and warrants further investigation.

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

“…Furthermore, it has been proven that extracellular matrix (ECM) proteins (mainly collagen, fibronectin, laminin and vitronectin) (Müller et al, 2006;Rico et al, 2009;Chen et al, 2018), their RGD sequence (Arg-Gly-Asp) (Mörke et al, 2017) and cytokines [e.g., basic fibroblast growth factor (bFGF)] (Cao et al, 2015) have the capacity to promote cell attachment.…”

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

“…The interaction results in the damage of blood cellular membrane or the erythrocytes lysis leading to the release of their intracellular hemoglobin. The amount of released hemoglobin should be small to ensure the absence of toxicity of the implanted material . Moreover, hemocompatibility of membranes in contact with bone tissue could affect osseointegration .…”

“…More recently, in addition to improved hemocompatibility, results showed that heparin could affect the functions of osteoblast cells, such as osteoblast‐like MC3T3‐E1 . For instance, Cao et al studied on the GBR membrane based on PCL containing basic fibroblast growth factor and heparin . They showed that heparin‐PCL membrane exhibited larger projected areas than PCL one which was appropriate for osteoblast like cells to attach, proliferate, and differentiate.…”

Polycaprolactone-Based Biomaterials for Guided Tissue Regeneration Membrane

“…The basic fibroblast growth factor-releasing heparin-conjugated PCL membrane has been successfully developed and exhibits biocompatibility. This basic fibroblast growth factor-releasing PCL membrane promotes human osteoblast-like cell attachment, proliferation and differentiation as compared with the naïve PCL membrane [79]. Metronidazole-incorporated PCL-based membranes decrease inflammatory response, determined in subcutaneous implantation model as compared to the unmodified PCL membrane [40,80].…”