Preparation and physicochemical characterization of biodegradable nerve guides containing the nerve growth agent sabeluzole

Verreck, Geert; Chun, Iksoo; Li, Yufu; Kataria, Ram; Zhang, Qiang; Rosenblatt, Joel; Decorte, Annelies; Heymans, Koen; Adriaensen, Jef; Bruining, Monique J.; Remoortere, Marie Van; Borghys, Herman; Meert, Theo; Peeters, Jef; Brewster, Marcus E.

doi:10.1016/j.biomaterials.2004.04.040

Cited by 82 publications

(49 citation statements)

References 34 publications

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“…Biodegradable conduits avoid the necessity of a second surgical operation and allow the delivery of incorporated Schwann cells or bioactive molecules during biodegradation (Ciardelli and Chiono 2006). Examples of bioresorbable polymers for nerve repair include aliphatic polyesters and copolyesters, such as poly(L-lactic acid) (PLLA) (Luciano et al 2000;Yang et al 2004), polyglycolic acid (PGA) (Nakamura et al 2004), poly(lactic acid-ε-caprolactone) (Den Dunnen et al 1998, poly(L-lactide-co-glycolide) (PLGA) (Bini et al 2004), poly(1,3-trimethylenecarbonate-ε-caprolactone) (Pego et al 2001) and poly(caprolactone) (PCL) (Bender et al 2004;Verreck et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Melt-extruded guides for peripheral nerve regeneration. Part I: Poly(ε-caprolactone)

Chiono

Vozzi

et al. 2009

Biomed Microdevices

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Melt-extruded guides for peripheral nerve repair based on poly(epsilon-caprolactone) (PCL) were realised and their physico-chemical properties were evaluated. Preliminarily, PCL cast films were found to support the attachment and proliferation of Neonatal Olfactory Bulb Ensheating Cells (NOBEC). S5Y5 neuroblastoma cells were cultured inside PCL guides in their uncoated form or coated with a non-specific adhesion protein (gelatin) and a specific peptide for nerve regeneration (poly(L-lysine)). Coating increased cell density (gelatin) and/or the cell density rate on substrates (poly(L-lysine); gelatin) as compared to uncoated guides. Various in vivo tests were carried out for the repair of small (0.5 cm), medium (1.5 cm) and long (4.5 cm) size defects in the peripheral nerves of Wistar rats. For the small nerve defects, uncoated and coated PCL guides were tested. Results from in vivo tests were subjected to histological examination after 45 days, 6 and 8 months postoperative for small, medium and large defects, respectively. Regeneration was found for small and medium size defects. For 0.5 cm defects, the coating did not affect regeneration significantly. Grip-tests also evidenced functional recovery for the 1.5 cm-long defects treated with PCL guides, after 6 months from implantation. On the other hand, mechanical stiffness of PCL conduits impaired the repair of 4.5 cm-long defects in 8-month period: the lack of flexibility of the guide to rat movements caused its detachment from the implant site. The research showed that PCL guides can be used for the successful repair of small and medium size nerve defects, with possible improvements by suitable bio-mimetic coatings.

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Section: Introductionmentioning

confidence: 99%

Melt-extruded guides for peripheral nerve regeneration. Part I: Poly(ε-caprolactone)

Chiono

Vozzi

et al. 2009

Biomed Microdevices

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“…An ideal neuron-supporting substrate should be biocompatible, neuroconductive, neuroinductive, biodegradable, flexible, harmless to the surrounding tissues, and resistant to structural collapse during implantation, as well as satisfy appropriate surface and mechanical properties [Verreck et al, 2005]. Although CHT and PLGA are biocompatible polymers that are extensively used for a range of biomedical applications and fulfill some of the above-stated properties, they have some disadvantages, which have to be overcome to make them suitable for neural tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Neuronal Differentiation Modulated by Polymeric Membrane Properties

Morelli

Piscioneri²,

Drioli³

et al. 2017

Cells Tissues Organs

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In this study, different collagen-blend membranes were successfully constructed by blending collagen with chitosan (CHT) or poly(lactic-co-glycolic acid) (PLGA) to enhance their properties and thus create new biofunctional materials with great potential use for neuronal tissue engineering and regeneration. Collagen blending strongly affected membrane properties in the following ways: (i) it improved the surface hydrophilicity of both pure CHT and PLGA membranes, (ii) it reduced the stiffness of CHT membranes, but (iii) it did not modify the good mechanical properties of PLGA membranes. Then, we investigated the effect of the different collagen concentrations on the neuronal behavior of the membranes developed. Morphological observations, immunocytochemistry, and morphometric measures demonstrated that the membranes developed, especially CHT/Col30, PLGA, and PLGA/Col1, provided suitable microenvironments for neuronal growth owing to their enhanced properties. The most consistent neuronal differentiation was obtained in neurons cultured on PLGA-based membranes, where a well-developed neuronal network was achieved due to their improved mechanical properties. Our findings suggest that tensile strength and elongation at break are key material parameters that have potential influence on both axonal elongation and neuronal structure and organization, which are of fundamental importance for the maintenance of efficient neuronal growth. Hence, our study has provided new insights regarding the effects of membrane mechanical properties on neuronal behavior, and thus it may help to design and improve novel instructive biomaterials for neuronal tissue engineering.

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“…The results from cell culture studies suggested that the scaffolds were non-toxic and capable of supporting fibroblast adhesion and proliferation [14]. However, the literature does not report examples of bioartificial blends between PCL and gelatin used as nerve guidance channels, whereas gelatin and PCL have separately been used for peripheral nerve regeneration [15][16][17]. One of gelatin drawbacks for tissue engineering applications is its solubility in aqueous media, therefore gelatin-containing structures for long-term biomedical applications need to be crosslinked.…”

Section: Introductionmentioning

confidence: 99%

Enzymatically‐Modified Melt‐Extruded Guides for Peripheral Nerve Repair

Chiono¹,

Ciardelli²,

Vozzi³

et al. 2008

Engineering in Life Sciences

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In this work, melt-extruded guides for peripheral nerve repair were produced, based on blends between poly-(e-caprolactone) and gelatin with a low gelatin amount (10 wt. %), with the aim of combining the good melt process ability of the synthetic polymer with the optimal biocompatibility of the natural polymer. In one case, a blend was produced between poly-(e-caprolactone) and gelatin previously crosslinked by transglutaminase, using a solution mixing technique. The blend was then melt-extruded obtaining nerve guidance channels. In another case, a blend between poly-(e-caprolactone) and uncrosslinked gelatin was first produced, then melt-extruded into tube-shaped manufacts. Finally, poly-L-lysine was grafted on the gelatin domains exposed on the inner tube surface using transglutaminase catalysis, with the aim to confer to the channel guide a specific signaling for nerve cells attachment, proliferation and migration. Scanning electron microscopy (SEM) and Fourier transform infrared-attenuated total reflectance spectroscopy (FTIR-ATR) coupled with Chemical Imaging analysis showed that the obtained binary blends were poor compatible, however, appropriate mixing techniques might lead to an homogeneous distribution of gelatin domains within the poly-(e-caprolactone) matrix. Confocal microscopy (CM) was applied as a powerful tool to study the accessibility of mTGase towards gelatin substrates, using suitable model lysine-rich peptides (FITC-labeled KKKKGY). Confocal microscopy also gave a confirmation of poly-L-lysine enzymatic grafting on the exposed gelatin domains on the inner blend tube surface. In vitro cell tests using S5Y5 neuroblastoma cells showed that the produced nerve guides were biocompatible, however, gelatin was confirmed to be a non-specific protein for the attachment and proliferation of nerve cells. On the other hand, poly-L-lysine functionalization of the inner tube surface greatly improved the in-vitro cell response.

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Preparation and physicochemical characterization of biodegradable nerve guides containing the nerve growth agent sabeluzole

Cited by 82 publications

References 34 publications

Melt-extruded guides for peripheral nerve regeneration. Part I: Poly(ε-caprolactone)

Melt-extruded guides for peripheral nerve regeneration. Part I: Poly(ε-caprolactone)

Neuronal Differentiation Modulated by Polymeric Membrane Properties

Enzymatically‐Modified Melt‐Extruded Guides for Peripheral Nerve Repair

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