Preparation and physical‐biological characterization on titanium doped fluorophosphate nanobioglass: Bone implants

Sankaralingam, Pugalanthi Pandian; Sakthivel, Poornimadevi; Subbiah, Priscilla Andinadar; Rahumathulla, Jenifabegum; Thangavel, Vijayakumar Chinnaswamy

doi:10.1002/ces2.10078

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…Computational simulations complement our previous study on the interaction of a series of amino acids with a model rutile (110) surface [29] and support the experimental findings of a signifi-cant suppression of amino acids adsorption on rutile by Tris. Despite growing concerns regarding the reliability of Trisbuffered SBF solutions for in vitro testing of biomaterials, and the effort of various groups to present refined protocols [24], in vitro bioactivity tests are still very commonly performed using Tris [30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51]. Our results highlight and quantify the so-far mostly overlooked role of Tris and should motivate the scientific community to establish in vitro testing protocols that are free of Tris-induced artefacts.…”

Section: Introductionmentioning

confidence: 80%

Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

YazdanYar¹,

Buswell²,

Pantaloni³

et al. 2021

Preprint

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Tris(hydroxymethyl)aminomethane (Tris) has been used as the buffer in bioactivity testing for over two decades and has become a standard choice for the scientific community. While it is believed to be non-interacting, the extent of its interactions with titanium oxide surfaces has not been systematically studied. Here, we use experimental (zeta potential measurements) and computational (molecular dynamics) approaches to evaluate the interaction of Tris with a rutile surface and how it affects the adsorption of other molecules relevant in biomedical in vitro testing. We show that the interaction of Tris with the rutile surface is strong and significantly affects the interaction of other organic residues with the surface. These strong interactions are compounded by the Tris concentration in the in vitro testing protocol which is much higher compared to other components. Our findings indicate that the kinetics observed in in vitro tests will be strongly influenced by the presence of Tris as a buffering agent when compared to the natural CO 2 buffer in blood. These results reveal that considering the so-far neglected active role of Tris in in vitro testing is critically needed and that in vitro protocols using CO 2 partial pressure as the buffering agent should yield more reliable results.

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Section: Introductionmentioning

confidence: 80%

Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

YazdanYar¹,

Buswell²,

Pantaloni³

et al. 2021

Preprint

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show abstract

“…Yang et al, 2011. [Colour figure can be viewed at wileyonlinelibrary.com] engineered to release fluoride ions at a controlled rate, increasing proliferation and osteogenesis of MC3T3 cells in vitro (Borkowski et al, 2020). Similarly, titanium containing fluoride-doped phosphate nanobioglasses enhanced osteogenesis in vitro resulting in higher amounts of bone formation in vivo (Sankaralingam et al, 2021).…”

Section: Scaffold Dopantsmentioning

confidence: 99%

Therapeutic approaches to activate the canonical Wnt pathway for bone regeneration

Nelson

Fontana

Miclau

et al. 2022

J Tissue Eng Regen Med

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Activation of the canonical Wingless‐related integration site (Wnt) pathway has been shown to increase bone formation and therefore has therapeutic potential for use in orthopedic conditions. However, attempts at developing an effective strategy to achieve Wnt activation has been met with several challenges. The inherent hydrophobicity of Wnt ligands makes isolating and purifying the protein difficult. To circumvent these challenges, many have sought to target extracellular inhibitors of the Wnt pathway, such as Wnt signaling pathway inhibitors Sclerostin and Dickkopf‐1, or to use small molecules, ions and proteins to increase target Wnt genes. Here, we review systemic and localized bioactive approaches to enhance bone formation or improve bone repair through antibody‐based therapeutics, synthetic Wnt surrogates and scaffold doping to target canonical Wnt. We conclude with a brief review of emerging technologies, such as mRNA therapy and Clustered Regularly Interspaced Short Palindromic Repeats technology, which serve as promising approaches for future clinical translation.

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Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

et al. 2021

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Preparation and physical‐biological characterization on titanium doped fluorophosphate nanobioglass: Bone implants

Cited by 3 publications

References 37 publications

Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

Therapeutic approaches to activate the canonical Wnt pathway for bone regeneration

Interactions of Tris with rutile surfaces and consequences for in vitro bioactivity testing

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